Method Development Of Active Solvent Modulation (Asm) - Agilent Technologies 1290 Infinity II 2D-LC User Manual

7

Method Development of Active Solvent Modulation (ASM)

Method Development of Active Solvent Modulation (ASM)

Method Development of Active Solvent
Modulation (ASM)
ASM method development helps finding the optimal dilution of 1D solvents in the
sample loop for best 2D resolution at lowest cycle time.
After switching on the ASM functionality (see
execute the steps in the following order:
•
•
•
•
2D-LC User Guide
"Optimize the Dilution by Using ASM Capillaries"
"Optimize the Sample Loop Flush"
"Include the ASM Phase to the 2D Gradient"
"Optimize Dilution Through Method Settings"
"Method Parameters"
on page 188
on page 189
on page 190
on page 191
on page 187),
186