Optimize Dilution Through Method Settings - Agilent Technologies 1290 Infinity II 2D-LC User Manual

7 Method Development of Active Solvent Modulation (ASM)

Optimize Dilution Through Method Settings

Optimize Dilution Through Method Settings

Figure 104 Optimizing separation using a lower percentage of B for the ASM and column
For optimizing separation, you may use a lower percentage of B for the ASM
phase and column equilibration phase compared to the original gradient for
increasing dilution before the
If for example the original analytical gradient started at 20 % B, you may use an
ASM phase of for example 2 % B for diluting
ASM phase by changing the gradient start condition and adding a line to the 2D
gradient table for the ASM phase. The starting point for the analytical gradient
does not change. The solvent composition of the equilibration phase is
automatically reduced to the start condition.
Apply high-resolution sampling with small cut sizes. Small cut sizes reduce the
transfer of solvent volume from
compatibility and 2D resolution.
2D-LC User Guide
equilibration phase (example)
2
D column.
1
D solvent more strongly during the
1 2
D to D, which can further improve solvent
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