Include The Asm Phase To The 2D Gradient - Agilent Technologies 1290 Infinity II 2D-LC User Manual

7 Method Development of Active Solvent Modulation (ASM)

Include the ASM Phase to the 2D Gradient

Include the ASM Phase to the
Figure 103 Programming the
The dilution during the ASM phase takes time. That's why the ASM phase shifts
the analytical gradient start.
An ASM phase of, for example, 0.68 min (based on selected ASM capillary, flush
factor and
without ASM
•
•
•
•
This rule is true for shifted gradient steps as well (if applicable).
2D-LC User Guide
2
D flow rate) shifts all times by 0.68 min compared to a
Gradient ends later
2
D cycle time is increased accordingly
One line is added to the gradient table for the ASM phase
All analytical gradient times shift by 0.68 min
2
D gradient table (example)
2
D Gradient
2
D gradient
190