Run The Checkout Familiarization Procedure For Comprehensive (Lcxlc) - Agilent Technologies 1290 Infinity II 2D-LC User Manual

8 Run the System
Run the Experiment
Run the Checkout Familiarization Procedure for
Comprehensive (LCxLC)
To run the checkout, various hardware configurations are possible, see
on page 56. Not all options can be shown. As example the
is used here.
To achieve optimal sensitivity, in comprehensive mode, especially for LC/MS
applications, the LC flow is often split prior to the mass spectrometer.
The following parameters have been optimized for this standard configuration.
Parameters can deviate slightly for your system. Run the experiment and
subsequently edit or optimize the method for your setup.
Table 24
Description (PN)
TEE, ST, 1/16 inch, Low Dead Volume
(0100-0969)
DAD Heat Exchanger Capillary 310 mm, 0.12 mm
i.d. (G1315-87339)
Capillary ST 0.075 mm x 500 mm, long socket
(5500-1205)
Capillary ST 0.075 mm x 250 mm, long socket
(5500-1206)
2D-LC User Guide
Example for a MS passive splitter setup (ratio 1:2)
Table 14
Usage
T-piece
2D detector connected to T-piece
Inlet of the LCMS source connected to the other
end of the T-piece
Remaining connection to the T-piece is used as
waste capillary
Table 5
on page 198
214