Operating Principle - Agilent Technologies 1290 Infinity II 2D-LC User Manual

2 Concepts of 2D-LC
Active Solvent Modulation (ASM)

Operating Principle

Figure 18 Operating principle with sample loop in flow
path (schematic view)
1
D Solvent in the sample loop is partially diluted by
2 *
from the D pump.
* 2 2
red: D solvent from D pump, blue: sample with
2D-LC User Guide
Figure 19 Operating principle with sample loop and ASM
capillary in parallel flow path (schematic view)
2 Introducing a parallel flow through an ASM capillary strongly
D solvent
1
dilutes D solvent with weaker
conditions focus the sample on the head of the
and therefore enable a good separation.
1
D solvent in sample loop
2
D solvent. These solvent
2
D column
*
37