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Agilent Technologies 1290 Infinity II User Manual

Agilent Technologies 1290 Infinity II User Manual

Evaporative light scattering detector
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Summary of Contents for Agilent Technologies 1290 Infinity II

  • Page 1 Agilent 1290 Infinity II Evaporative Light Scattering Detector User Manual Agilent Technologies...
  • Page 2 1987) or any equivalent agency regulation or contract clause. Use, duplication or dis- closure of Software is subject to Agilent Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will Agilent 1290 Infinity II ELSD User Manual...
  • Page 3 In This Book In This Book This manual contains information on: • Agilent 1290 Infinity II ELSD (G7102A) 1 Introduction to the Detector This chapter gives an introduction to the module, instrument overview and internal connectors. 2 Site Requirements and Specifications This chapter provides information on environmental requirements, physical and performance specifications.
  • Page 4 This chapter describes the maintenance of the ELSD. 10 Parts and Materials for Maintenance This chapter provides information on parts for maintenance. 11 Appendix This chapter provides addition information on safety, legal and web. Agilent 1290 Infinity II ELSD User Manual...

  • Page 5: Table Of Contents

    PC and Agilent OpenLAB ChemStation and EZChrom Setup 5 Using the Module Before Using the Detector Instrument Controls Operational Parameters Controlling the ELSD During an Injection Controlling the ELSD within ChemStation or EZChrom General Considerations Agilent 1290 Infinity II ELSD User Manual...
  • Page 6 Putting the Instrument into Storage Updating Detector Firmware 10 Parts and Materials for Maintenance Identifying Parts and Materials 11 Appendix General Safety Information Waste Electrical and Electronic Equipment Directive Radio Interference Agilent Technologies on Internet Agilent 1290 Infinity II ELSD User Manual...

  • Page 7: Introduction To The Detector

    Agilent 1290 Infinity II ELSD User Manual Introduction to the Detector Introduction to the ELSD System Overview Basic Principles of Operation Operational Parameters Overview of ELS Detector This chapter gives an introduction to the module, instrument overview and internal connectors.

  • Page 8: Introduction To The Elsd

    The Agilent 1290 Infinity II ELSD may be used alone, or as one of several detectors in a GPC or HPLC system. As the solvent or eluent is evaporated in the course of the analysis, the Agilent 1290 Infinity II ELSD must be the last in series if used in conjunction with other detectors.

  • Page 9: System Overview

    By adding a stream of dry nitrogen (evaporation gas) at the entrance of the evaporator tube less volatile solvents (for example, water) are easily evaporated. This evaporation gas is controlled by the user and facilitates sub- ambient operation. Agilent 1290 Infinity II ELSD User Manual...
  • Page 10 This scattered light enters the optical aperture of the detection system and generates a signal response from the photodiode in real time. The quantity of light detected is dependent on the solute concentration and solute particle size distribution. Figure 1 Principles of Operation Agilent 1290 Infinity II ELSD User Manual...
  • Page 11 1.3 and 1.5. Changes in the refractive index within this range will not greatly affect the quantity of light reaching the detector. This accounts for similarities in the sensitivity of the instrument to various compounds. Agilent 1290 Infinity II ELSD User Manual...

  • Page 12: Operational Parameters

    Using this patented evaporation gas technology, a 20 µm droplet of water at 30 °C can be dried ca. 3x faster than just temperature alone. Using the Agilent 1290 Infinity II ELSD (G7102A), water can be evaporated as low as 20 °C, providing maximum sensitivity to thermally sensitive compounds.

  • Page 13: Overview Of Els Detector

    Introduction to the Detector System Overview Overview of ELS Detector Figure 3 ELSD overview (front) Front screen display Keypad Eluent inlet Solvent waste outlet Agilent 1290 Infinity II ELSD User Manual...
  • Page 14 Introduction to the Detector System Overview Figure 4 ELSD overview (rear) Vapor Sensor vent Serial RS232 port LAN connector Firmware button I/O connector (Remote Start input) Mains Switch Mains Input Gas Inlet port Exhaust port Agilent 1290 Infinity II ELSD User Manual...
  • Page 15 In order to make appropriate remote start and A/Z connections from a third party LC, a third N O T E party remote start cable for Dimension software (Remote start cable (PL0890-0350)) can be purchased from Agilent Technologies. Agilent 1290 Infinity II ELSD User Manual...
  • Page 16 Introduction to the Detector System Overview Agilent 1290 Infinity II ELSD User Manual...

  • Page 17: Site Requirements And Specifications

    Agilent 1290 Infinity II ELSD User Manual Site Requirements and Specifications Pre-installation Requirements Site Requirements Physical Specifications Performance Specifications Specifications Specification Conditions This chapter provides information on environmental requirements, physical and performance specifications. Agilent Technologies...

  • Page 18: Pre-Installation Requirements

    For a detailed description of the environmental and operating requirements of the ELSD, please refer to the Site Preparation Checklist. This manual will give you an overview of the ELS detector, and describe its operation in more detail. Agilent 1290 Infinity II ELSD User Manual...

  • Page 19: Site Requirements

    Site Preparation Checklist for more details. Laser Safety The Agilent 1290 Infinity II ELSD (G7102A) is classified as a "Laser Class 1" product (IEC825- 1, CFR1040.10 & 1040.11). During normal operation of the ELSD no laser light is accessible to the user.
  • Page 20 Never operate your instrumentation from a power outlet that has no ground connection. ➔ Never use a power cord other than the Agilent Technologies power cord designed for your region. Use of unsupplied cables WA R N I N G Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury.
  • Page 21 15 cm (5.9 inches) in the rear for air circulation and electric connections. If the bench shall carry a complete HPLC system, make sure that the bench is designed to bear the weight of all modules. The module should be operated in a horizontal position. Agilent 1290 Infinity II ELSD User Manual...

  • Page 22: Physical Specifications

    Up to 2000 m (6562 ft) Non-operating altitude Up to 4600 m (15091 ft) For storing the module Safety standards: Installation category II, Pollution degree 2 For indoor use only. IEC, CSA, UL Agilent 1290 Infinity II ELSD User Manual...

  • Page 23: Performance Specifications

    <1 mV/h under specified condtions. See Drift “Specification Conditions” on page 24. Operating Pressure 60 – 100 psi (4.1 – 6.9 bar) Eluent Flow range 0.2 – 5.0 mL/min Digital Output 10, 40 or 80 Hz (24 bit) Agilent 1290 Infinity II ELSD User Manual...

  • Page 24: Specification Conditions

    1 °C/h (1.8 °F/h). Turbulences around one minute or less can be ignored. ASTM measurements require that the detector should be turned on enough time before start of testing. Agilent 1290 Infinity II ELSD User Manual...

  • Page 25: Installing The Module

    Agilent 1290 Infinity II ELSD User Manual Installing the Module Unpacking the Module Delivery Checklist Installing the Module Connecting the Detector to your LC System Checking Operation of the ELS Detector This chapter gives information about the installation of your ELSD.

  • Page 26: Unpacking The Module

    Unpacking the Module Unpacking the Module If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment. "Defective on arrival" problems C A U T I O N If there are signs of damage, please do not attempt to install the module.

  • Page 27: Delivery Checklist

    Delivery Checklist Unpack the ELSD and accessories, and ensure that all parts and materials shown in the table below have been delivered with your module. Report any missing or damaged parts to your local Agilent Technologies sales and service office. Description...

  • Page 28: Installing The Module

    Though it will not cause any damage, if the solvent waste tube becomes submersed below N O T E the solvent level inside the waste container positive pressure will be exerted on the nebuliser chamber leading to excessive baseline noise. Agilent 1290 Infinity II ELSD User Manual...
  • Page 29 (ChemStation Rev B not supported) refer to the appropriate user guide supplied with the driver install. For further information on how to configure the TCP/IP settings on the detector, see N O T E chapter LAN Configuration. Agilent 1290 Infinity II ELSD User Manual...
  • Page 30 The liquid inlet port is connected directly to the nebuliser by a short length (130 mm) of capillary tube giving a delay volume from port to nebuliser tip of ~4 µL. 13 Turn on the source gas to a pressure of about 4.1 – 6.9 bar. Agilent 1290 Infinity II ELSD User Manual...

  • Page 31: Checking Operation Of The Els Detector

    5 Again check the baseline noise. Where noise and all other conditions are acceptable, the instrument is ready to begin work. Some volatile buffers can generate considerable offsets and increased noise levels. N O T E Agilent 1290 Infinity II ELSD User Manual...
  • Page 32 Installing the Module Installing the Module Agilent 1290 Infinity II ELSD User Manual...
  • Page 33 Agilent 1290 Infinity II ELSD User Manual LAN Configuration What You Have to Do First TCP/IP Parameter Configuration Configuration Using Instrument Front Panel Configuration Using Web Browser With Telnet PC and Agilent OpenLAB ChemStation and EZChrom Setup PC Setup for Local Configuration...

  • Page 34: Lan Configuration What You Have To Do First

    “Configuration Using Instrument Front Panel” on page 36). 2 Connect the instrument’s LAN interface to • the PC network card using a crossover network cable (point- to- point) • a hub switch using a standard LAN cable. Agilent 1290 Infinity II ELSD User Manual...

  • Page 35: Tcp/Ip Parameter Configuration

    If the detector is assigned a temporary IP address (e.g DHCP server) then communication N O T E with the PC may be prevented following a power cycle of the instrument. Table 5 Default STATIC/IP parameters IP address 192.168.254.27 Subnet Mask 255.255.255.0 Default Gateway 192.168.254.1 Agilent 1290 Infinity II ELSD User Manual...

  • Page 36: Configuration Using Instrument Front Panel

    8 To enter a new Gateway address use the up/down arrow keys. 9 When all values are populated press AZ/Stop to accept. The instrument will return an INVALID network setting message if the IP address and N O T E Gateway combination are incompatible. Agilent 1290 Infinity II ELSD User Manual...

  • Page 37: Configuration Using Web Browser

    IP address of the ELSD into the address bar (for example http://192.168.254.27). The default host name is configured to the detector’s serial number. N O T E If communication is successful, the following login screen will appear: Agilent 1290 Infinity II ELSD User Manual...
  • Page 38 The detector can be configured to • obtain an IP address automatically (DHCP), • use a static IP address, • change Hostname (Advanced Network settings). 4 To use a static IP address, select Use the following IP address. Agilent 1290 Infinity II ELSD User Manual...
  • Page 39 Contact your system administrator for assistance on configuring the detector’s network N O T E settings. Changes to the TCP/IP parameters, other than those described, could render the ELSD N O T E inoperable. Agilent 1290 Infinity II ELSD User Manual...

  • Page 40: With Telnet

    IP address (see Table 5 on page 35). If Telnet is not installed on your PC by default, you can turn on the feature via Windows N O T E Control Panel. Agilent 1290 Infinity II ELSD User Manual...
  • Page 41 Password:Agilent The login details are case sensitive. The password will be not visible on screen. N O T E 4 At the #> prompt type set network ? to view the available commands. Agilent 1290 Infinity II ELSD User Manual...
  • Page 42 6 When you have finished typing the configuration parameters, type quit and press Enter to exit. 7 Restart the module for the new TCP/IP settings to be implemented. Agilent 1290 Infinity II ELSD User Manual...

  • Page 43: Pc And Agilent Openlab Chemstation And Ezchrom Setup

    PC to match the modules default parameters in a local configuration using a cross- over LAN cable (see Table 5 on page 35). 1 Open the Local Area Connection Properties and select Internet Protocol (TCP/IP). Then click on Properties. Agilent 1290 Infinity II ELSD User Manual...
  • Page 44 LAN Configuration PC and Agilent OpenLAB ChemStation and EZChrom Setup 2 Select Alternative Configuration. Agilent 1290 Infinity II ELSD User Manual...
  • Page 45 3 Enter the following IP and subnet mask address for the PC: • IP address: 192.168.254.1 • Subnet mask: 255.255.255.0 4 Click OK to save the configuration. It can take several seconds for the alternative network settings to take effect. N O T E Agilent 1290 Infinity II ELSD User Manual...

  • Page 46: Agilent Chemstation Setup

    PC and Agilent OpenLAB ChemStation and EZChrom Setup Agilent ChemStation Setup This procedure describes the Agilent OpenLAB ChemStation setup for the 1290 Infinity II ELSD detector (G7102A) using LAN as the communication method. 1 Open the Instrument Configuration window and select 1290 ELSD from the Configurable Modules window.
  • Page 47 LAN Configuration PC and Agilent OpenLAB ChemStation and EZChrom Setup 4 Select IP Address and enter the module’s IP address. Select Hostname and enter the instrument’s serial number. Agilent 1290 Infinity II ELSD User Manual...
  • Page 48 N O T E the software might be prevented following a power cycle of the instrument. Recommend using a static IP address to ensure communication with the software is preserved after power cycle. Agilent 1290 Infinity II ELSD User Manual...

  • Page 49: Using The Module

    Agilent 1290 Infinity II ELSD User Manual Using the Module Before Using the Detector Instrument Controls Display Screen Keypad Main menu bar Sub-Menu Screen Status Mode Error conditions Clearing an Error Operational Parameters Method Evaporator Temperature Nebuliser Temperature Evaporator Gas Flow...

  • Page 50: Before Using The Detector

    Before Using the Detector Before Using the Detector On start- up of the Agilent 1290 Infinity II ELSD (G7102A), either Serial or LAN must be selected as the method of communication. If Serial communication is selected, then no further configuration is required.

  • Page 51: Instrument Controls

    ELSD at any time, unless a timetable is running. If the AZ/Stop key is pressed during an active timetable, the timetable will stop running and the ELSD will revert to STANDBY mode. Agilent 1290 Infinity II ELSD User Manual...

  • Page 52: Main Menu Bar

    PWR MODE Set Mode of ELSD when powered up Set Data Output Rate Displays the instruments TCP/IP settings With serial connection option displays “Instrument Configured for RS-232” Figure 8 G7102A sub menu screen Agilent 1290 Infinity II ELSD User Manual...

  • Page 53: Status Mode

    ➔ The solvent pump must be turned off if the ELSD is going to be left in Standby mode longer than 15 minutes to prevent solvent flooding the detector. Agilent 1290 Infinity II ELSD User Manual...

  • Page 54: Error Conditions

    Once the source of the problem has been corrected, select RUN mode to put the ELSD back into its operational state. If the problem has not been rectified the ELSD will repeatedly error when RUN mode is selected. Agilent 1290 Infinity II ELSD User Manual...

  • Page 55: Operational Parameters

    80 – 90 °C. If the compound is semi- volatile, or has a low molecular weight, e.g. pharmaceutical drug, then the evaporator temperature should be set between 20 – 30 °C. Agilent 1290 Infinity II ELSD User Manual...

  • Page 56: Nebuliser Temperature

    (e.g. 1.6 – 1.8 SLM). The evaporation gas range for both models is: 0.9 – 3.25 SLM (0.05 increments). The default value is 1.6 SLM. Agilent 1290 Infinity II ELSD User Manual...

  • Page 57: Response Time (Smoothing)

    The data rate is stored in memory and is retained even after a power on/off cycle. The default value is 40 Hz. All ELSD conditions are retained on power cycling of the ELSD detector. N O T E Agilent 1290 Infinity II ELSD User Manual...

  • Page 58: Lan (Tcp/Ip Settings)

    This screen displays the ELSD's current configured TCP/IP settings. See chapter LAN Configuration for details on how to set these values. With serial connection screen option displays “Instrument Configured for RS- 232”. Figure 9 TCP/IP settings Agilent 1290 Infinity II ELSD User Manual...

  • Page 59: Controlling The Elsd During An Injection

    The DIMENSION software package allows you to create a timetable on a PC, which can subsequently be downloaded to the ELSD for later use. The DIMENSION software also allows you to clear a time table from the ELSD detector memory. Agilent 1290 Infinity II ELSD User Manual...

  • Page 60: Starting And Stopping Real-Time Control

    During an active timetable, where the evaporator temperature is being controlled, the status of the instrument will change from READY to NOT READY. This is normal behavior and will not affect the running of the timetable. Agilent 1290 Infinity II ELSD User Manual...
  • Page 61 The AZ/Stop button on the instrument’s front keypad can be used to interrupt the active timetable while it is running. When the AZ/Stop button is pressed the timetable is stopped with the instrument put into STANDBY mode. Agilent 1290 Infinity II ELSD User Manual...

  • Page 62: Controlling The Elsd Within Chemstation Or Ezchrom

    ELSD Driver is supplied on the CD supplied with the detector. For further information on how to install and configure the ELSD driver refer to the appropriate user guide supplied with the driver install. Agilent 1290 Infinity II ELSD User Manual...

  • Page 63: General Considerations

    The ELS Detector is a destructive technique and must be placed last when used in series with other detectors, or used in conjunction with a flow splitter for semi- preparative applications, where partial sample collection is required. Agilent 1290 Infinity II ELSD User Manual...

  • Page 64: Solvent Recommendations

    Amino columns used with aqueous mobile phase are particularly prone to this type of shedding and should be checked regularly. To minimize column breakdown always follow the manufacturer's instruction supplied with the column. Agilent 1290 Infinity II ELSD User Manual...
  • Page 65 8.2 – 10.2 Ammonium Acetate 3.8 – 5.8 8.2 – 10.2 Ammonium Bicarbonate 6.8 – 11.3 10.3 Ion-Pair Reagent Pentafluoropropionic acid (PFPA) ~0.6 Heptafluorobutyric acid (HFBA) ~0.6 Nonafluoropropionic acid (NFPA) ~0.6 Tridecafluoroheptanoic acid ~0.6 Agilent 1290 Infinity II ELSD User Manual...

  • Page 66: Transferring Elsd Temperature Methods

    G7102A ELSD is not valid and the only way to ensure that the detector will provide the optimum analyte signal- to- noise is to follow the guidelines outlined in “Operational Parameters” on page 12. Agilent 1290 Infinity II ELSD User Manual...

  • Page 67: Optimizing Performance

    Agilent 1290 Infinity II ELSD User Manual Optimizing Performance Do’s and Don’ts of ELS Detection Location of the Detector Module Pumping systems Mobile phase priming This chapter gives hints on how to optimize the performance or use additional devices. Agilent Technologies...

  • Page 68: Do's And Don'ts Of Els Detection

    NEVER allow the solvent waste outlet tube to become immersed in the waste solvent. ➔ When placing more than one HPLC detector in series, always place the ELS detector last. ➔ Only use volatile mobile phase additives. Agilent 1290 Infinity II ELSD User Manual...

  • Page 69: Location Of The Detector Module

    The ELS detector can be placed within 2 meters of an extraction unit, using the exhaust tube provided. Agilent 1290 Infinity II ELSD User Manual...

  • Page 70: Pumping Systems

    A backpressure regulator maybe necessary on certain pumps in order to minimize pulsation. This can also be achieved by the column itself or a coil of 0.127 mm (0.005 in) ID tubing placed between the pump and the injector/injection valve. Agilent 1290 Infinity II ELSD User Manual...

  • Page 71: Mobile Phase Priming

    LC system be performed without the ELS detector attached to prevent non- volatile impurities contaminating the ELS detector. The mobile phase should be degassed and filtered, either by sparging with Helium or using an on- line degasser. Agilent 1290 Infinity II ELSD User Manual...
  • Page 72 Optimizing Performance Mobile phase priming Agilent 1290 Infinity II ELSD User Manual...

  • Page 73: Troubleshooting And Diagnostics

    Agilent 1290 Infinity II ELSD User Manual Troubleshooting and Diagnostics Troubleshooting Troubleshooting an HPLC System General Problems Baseline noise Baseline spikes Low sensitivity Spiky peak tops but flat baseline Large Baseline offset Peak tailing Instrument Fails to zero No power...

  • Page 74: Troubleshooting

    Troubleshooting and Diagnostics Troubleshooting Troubleshooting If a problem is encountered Agilent Technologies advises that the troubleshooting section should be followed first to resolve the problem. If there is an error or fault and you follow the recommended course of action and the result is not satisfactory, then please direct the matter to Agilent Technologies or your local distributor.

  • Page 75: Troubleshooting An Hplc System

    Begin troubleshooting by adding the pump to the ELS Detector first and finish by adding the column last. If another type of detector is available use it before the ELS Detector to aid in troubleshooting. Agilent 1290 Infinity II ELSD User Manual...

  • Page 76: General Problems

    • Increase the back pressure on the pump by fitting a back pressure column between the pump and the injection valve • Use a pulse dampener directly after the pump in the system Agilent 1290 Infinity II ELSD User Manual...

  • Page 77: Baseline Spikes

    Clean contamination from widows in optical Optical chamber is contaminated chamber Stop the eluent flow and increase the Diffuser saturated with solvent evaporator temperature to maximum. Increase the flow rate to 2.8 SLM and wait 1 h Agilent 1290 Infinity II ELSD User Manual...

  • Page 78: Spiky Peak Tops But Flat Baseline

    High concentration of non-volatile buffer or unstabilised solvent or a more volatile buffer stabiliser (ammonium acetate or ammonium formate) Perform cleaning procedure Contaminated diffuser Refer to local distributor or Agilent Technologies Optics Heater failed Agilent 1290 Infinity II ELSD User Manual...

  • Page 79: Peak Tailing

    No power Probable cause Suggested actions Attach mains lead to socket and inlet on rear of Mains lead not connected instrument Replace fuse Fuse failure Call Agilent Service representative Power supply failure Agilent 1290 Infinity II ELSD User Manual...

  • Page 80: No Response (Completely Flat Baseline)

    Display not on, but power connected Probable cause Suggested actions Refer to local distributor or Agilent Instrument Power Supply Technologies. Refer to local distributor or Agilent Faulty display Technologies. Agilent 1290 Infinity II ELSD User Manual...

  • Page 81: Cooled Evaporator Temperature Reads Zero At Start-Up And Cannot Be Changed

    Probable cause Suggested actions Move detector to laboratory where ambient Ambient lab temperature too high temperature is <25 °C Consult Agilent Technologies or your local Faulty Peltier cooler unit agent if problem persists Agilent 1290 Infinity II ELSD User Manual...

  • Page 82: High Back-Pressure From Detector

    Troubleshooting and Diagnostics General Problems High back-pressure from detector Probable cause Suggested actions Manually syringe water into ELSD front Inlet Nebuliser or nebuliser inlet tube blocked port to remove obstruction Agilent 1290 Infinity II ELSD User Manual...

  • Page 83: Error Information

    Agilent 1290 Infinity II ELSD User Manual Error Information What Are Error Messages Module Specific Error Messages Internal temperature exceeded lower limit Internal temperature exceeded upper limit On-board Vapor sensor failed Rear Vapor sensor failed Vapor detected Leak detected Fan Failed...

  • Page 84: What Are Error Messages

    (flow path) failure occurs which requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary). In the event of such a failure, the ELSD alarms with an error message displayed. Agilent 1290 Infinity II ELSD User Manual...

  • Page 85: Module Specific Error Messages

    Air temperature inside the instrument is >40 °C Probable cause Suggested actions • Decrease ambient temperature where The environmental temperature is outside detector is located the specified operating limits of the instrument • Move the detector to a cooler location Agilent 1290 Infinity II ELSD User Manual...

  • Page 86: On-Board Vapor Sensor Failed

    Remove any source of solvent vapors close to the External vapor being drawn into unit module Please contact your Agilent service representative Solvent leak inside unit Fit black exhaust tube Exhaust tube not fitted Agilent 1290 Infinity II ELSD User Manual...

  • Page 87: Leak Detected

    Thermal shut- down of the main cooling fan Probable cause Suggested actions Please contact your Agilent service Fan cable disconnected. representative. Please contact your Agilent service Defective fan. representative. Please contact your Agilent service Defective main board. representative. Agilent 1290 Infinity II ELSD User Manual...

  • Page 88: Fan Stopped

    Nebuliser temperature exceeded threshold after stabilizing Probable cause Suggested actions Please contact your Agilent service Defective thermocouple representative Please contact your Agilent service Defective nebuliser heater representative Please contact your Agilent service Defective main board. representative. Agilent 1290 Infinity II ELSD User Manual...

  • Page 89: Evaporator Temperature Limit Exceeded

    Ensure the gas inlet pressure is above 60 psi Insufficient gas inlet pressure Please contact your Agilent service Defective mass flow controller representative Check flow controller cable Gas line not connected or Mass disconnected Agilent 1290 Infinity II ELSD User Manual...

  • Page 90: Invalid Nebulizer Temperature

    Please contact your Agilent service Defective evaporator heater representative Ensure the evaporator heater is connected RTD Block to TEC board for cooled ELSD not correctly connected to the main board Agilent 1290 Infinity II ELSD User Manual...

  • Page 91: Fan Failed On Cooled Evaporator

    • Please contact your Agilent service representative • Exchange Peltier assembly Peltier assembly not connected to main board • Please contact your Agilent service representative Please contact your Agilent service Defective main board. representative. Agilent 1290 Infinity II ELSD User Manual...

  • Page 92: Laser Temperature Out Of Range

    • Please contact your Agilent service representative Please contact your Agilent service representative Interlock not connected to main board Please contact your Agilent service representative Interlock wiring is faulty Please contact your Agilent service representative. Defective main board. Agilent 1290 Infinity II ELSD User Manual...

  • Page 93: Communication To Laser Failed

    Suggested actions Please contact your Agilent service Leak sensor not connected to the main representative. board. Please contact your Agilent service Defective leak sensor. representative. Please contact your Agilent service Defective main board. representative. Agilent 1290 Infinity II ELSD User Manual...
  • Page 94 Error Information Module Specific Error Messages Agilent 1290 Infinity II ELSD User Manual...

  • Page 95: Maintenance

    Agilent 1290 Infinity II ELSD User Manual Maintenance Introduction to Maintenance Cautions and Warnings Cleaning the Module Inspection of Cables Drying the Diffuser Cleaning the Nebuliser Cleaning Evaporator Tube Putting the Instrument into Storage Updating Detector Firmware This chapter describes the maintenance of the ELSD.

  • Page 96: Introduction To Maintenance

    There are no user serviceable parts inside the instrument. Unauthorized access to the instrument will invalidate the instrument warranty. Information for Service Personnel Please note that this instrument is double fused. The following fuses are fitted: • 2x T2A H 250 V Agilent 1290 Infinity II ELSD User Manual...

  • Page 97: Cautions And Warnings

    ➔ Be sure to hold the board by the edges, and do not touch the electrical components. Always use ESD protection (for example, an ESD wrist strap) when handling electronic boards and components. Agilent 1290 Infinity II ELSD User Manual...

  • Page 98: Cleaning The Module

    Switch off and disconnect power cord from instrument before cleaning. ➔ Do not use an excessively damp cloth during cleaning. ➔ Drain all solvent lines before opening any fittings. ➔ Allow the instrument to dry off completely before reconnecting power. Agilent 1290 Infinity II ELSD User Manual...

  • Page 99: Inspection Of Cables

    Inspection of Cables Inspection of Cables Periodically inspect the connecting cables for signs of physical damage caused by abrasion, solvent spillage, impact etc. Replace damaged cables, particularly the power cord, if any damage is observed. Agilent 1290 Infinity II ELSD User Manual...

  • Page 100: Drying The Diffuser

    The diffuser will be dried out and the instrument ready to use after approximately 1 h under these conditions. 2 Reset the instrument to the correct operating conditions and allow to stabilize before continuing. Agilent 1290 Infinity II ELSD User Manual...

  • Page 101: Cleaning The Nebuliser

    It is not possible to pump solvent into the ELSD if the nebuliser becomes completely N O T E blocked. It is strongly recommended to regularly flush the ELSD with water to keep the nebuliser clear of obstruction. Agilent 1290 Infinity II ELSD User Manual...

  • Page 102: Cleaning Evaporator Tube

    40 h of use as a preventative routine. It is also recommended to clean the unit following the use of buffers. If cleaning the unit does not cure the problems then consult Agilent Technologies for further assistance.

  • Page 103: Putting The Instrument Into Storage

    Cover the waste tube with tissue paper to collect any acetone residue. 8 Plug the exhaust, waste tubes and solvent inlet with the plastic caps provided. Agilent 1290 Infinity II ELSD User Manual...

  • Page 104: Updating Detector Firmware

    Current firmware file Parts required Description Serial cable (supplied with instrument) Only Firmware versions v30.42 or later are compatible with the 1290 Infinity II ELSD. N O T E To upgrade/downgrade the modules firmware carry out the following steps: 1 Connect the detector to a PC, via the serial port on the rear of the module, using a RS232 cable.
  • Page 105 5 Set the Serial port to the parameters shown below and click OK. The Transmit delay values should be set according to the table below: Table 8 Transmit delay values Firmware type msec/char msec/line Main Safety Laser Agilent 1290 Infinity II ELSD User Manual...
  • Page 106 When in Service mode, the Tera Term displays the bootloader version and firmware menu. 7 Select the firmware you wish to upgrade/downgrade by pressing the appropriate number (e.g. press 1 to upgrade the main control firmware). Agilent 1290 Infinity II ELSD User Manual...
  • Page 107 8 Press U to begin the update process. 9 To choose the firmware file, select the Send File option from the File menu in the toolbar and navigate to the folder where the file is located. Agilent 1290 Infinity II ELSD User Manual...
  • Page 108 11 On successful completion of the file transfer, the software will display a Flash memory updated ok message. 12 Power cycle the module and check that the firmware version displayed on the ELSD front panel at boot- up is correct. Agilent 1290 Infinity II ELSD User Manual...

  • Page 109: Parts And Materials For Maintenance

    Agilent 1290 Infinity II ELSD User Manual Parts and Materials for Maintenance Identifying Parts and Materials This chapter provides information on parts for maintenance. Agilent Technologies...

  • Page 110: Identifying Parts And Materials

    ELSD Air Adapter Kit 8121-0008 LAN shielded cable G4260-60005 1200 Series to ELSD Trigger Cable PL0890-0350 Remote start cable (3rd party cable for Dimension software) G7102-90000 Agilent 1290 Infinity II Evaporative Light Scattering Detector User Manual Agilent 1290 Infinity II ELSD User Manual...

  • Page 111: Appendix

    Agilent 1290 Infinity II ELSD User Manual Appendix General Safety Information Waste Electrical and Electronic Equipment Directive Radio Interference Agilent Technologies on Internet This chapter provides addition information on safety, legal and web. Agilent Technologies...

  • Page 112: General Safety Information

    External Laser warning label located on rear of detector Internal Laser warning label located on light source Internal Laser beam label located on light source Agilent 1290 Infinity II ELSD User Manual...
  • Page 113 Agilent Technologies assumes no liability for the customer’s failure to comply with these requirements. Ensure the proper usage of the equipment.
  • Page 114 When working with solvents, observe appropriate safety procedures (for example, goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet by the solvent vendor, especially when toxic or hazardous solvents are used. Agilent 1290 Infinity II ELSD User Manual...

  • Page 115: Waste Electrical And Electronic Equipment Directive

    Monitoring and Control Instrumentation product. Do not dispose of in domestic household waste N O T E To return unwanted products, contact your local Agilent office, or see www.agilent.com more information. Agilent 1290 Infinity II ELSD User Manual...

  • Page 116: Radio Interference

    Appendix Radio Interference Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Agilent 1290 Infinity II ELSD User Manual...

  • Page 117: Agilent Technologies On Internet

    Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Agilent 1290 Infinity II ELSD User Manual...
  • Page 118 Agilent 1290 Infinity II ELSD User Manual...
  • Page 119 MAC address TCP/IP parameter configuration pre-installation using front panel main menu bar requirements using web browser method with telnet preparation loading sample TCP/IP settings local configuration priming temperature error pump nebuliser Agilent 1290 Infinity II ELSD User Manual...
  • Page 120 Index temperature methods transfer temperature troubleshooting error messages HPLC system unpacking vapor detected vapor sensor error voltage range waste electrical and electronic equipment WEEE directive weight Agilent 1290 Infinity II ELSD User Manual...
  • Page 121 Index Agilent 1290 Infinity II ELSD User Manual...
  • Page 122 In This Book This manual contains information on the Agilent 1290 Infinity II Evaporative Light Scattering Detector (G7102A). The manual describes the following: • Introduction • Site Requirements and Specifications • Installation • Using • Optimizing • Troubleshooting and Diagnostics •...

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G7102a

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