Setup Second Dimension Gradient With The Graphical User Interface - Agilent Technologies 1290 Infinity II 2D-LC User Manual

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6
Method Parameters

Setup Second Dimension Gradient with the Graphical User Interface

Setup Second Dimension Gradient with the
Graphical User Interface
The user can graphically set up the
(%B) value, the
Analytical
You can change or adjust the values of the Analytical Gradient graphically. In the preview, select one of the
Gradient
black bullets with the mouse and move the bullet up and down. These changes will automatically update the
Analytical Gradient settings in the table. By double clicking on the line in the Analytical Gradient window, you
can set more anchor points to adjust the analytical gradient even better.
Gradient Shift
The setup of the shifted gradient can also be done graphically. If you double click one of the black bullets in
1
D Time
the preview window, you will get a dotted line, which represents the shifted
2
(Shifted
D
up and down, you can align the shifted
gradient)
double clicking on the dotted line again, you can set more anchor points to adjust the shifted gradient even
better. These changes will automatically update the Gradient Shift
NOTE
Within a HiRes series shifted gradients are prohibited, but shifts are allowed from HiRes series to series.
2D-LC User Guide
2
2
D stop time, and the modulation (repetition) time.
2
D gradient to the different solvent composition from the
D gradient including the initial composition
2
D gradient. By moving the bullet
1
D Time settings in the table.
1
D run. By
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