Features - Agilent Technologies 1290 Infinity II 2D-LC User Manual

1 Introduction

Features

Features
Agilent InfinityLab 2D-LC Solutions offers following key features:
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2D-LC User Guide
Agilent 2D-LC is based on 1290 Infinity II Systems with UHPLC performance,
fast gradients, high sensitivity and excellent robustness
Dedicated 2D-LC valves use completely symmetric flow paths for
reproducible retention times and peak areas.
A wide range of modules can be used in both dimensions. Third-party
detectors are supported via UIB II including the sue of compatible detectors
for data analysis
Powerful Agilent 2D-LC software is available for OpenLab CDS, MassHunter
and ChemStation. Measurements can be set up easily with a few mouse
clicks: Starting with a 1D measurement, choose spots where you want to
increase resolution and draw your second dimension gradient.
Agilent 2D-LC instrument control is fully automated and eliminates the need
for tedious manual valve programming. Separation in the first and second
dimension are completely independent by using Agilent multiple heart-cutting
valves for highest storage capacity for up to 12 cuts at one time, fast and
parallel analysis.
High-resolution sampling is available for flexibly analyzing short cuts to broad
peaks while retaining first dimension separation. By analyzing complete
peaks, highly reproducible quantitative measurements can be achieved.
Multiple heart-cutting and High-resolution sampling can be combined
arbitrarily within one run
Shifted gradients, which can be edited graphically or numerically, maximize
the available 2D separation space for highest peak capacity and fastest
analysis
Dedicated flush gradients are available for fast analysis and minimum
carryover
Cuts can be defined in time-based mode for highly reproducible
measurements or peak-based mode for variable retention times or unknown
samples. Even in peak-based mode, both first and second dimension
detectors provide complete chromatograms.
Dynamic peak parking combines time- and peak-based approaches for
dealing with shifting first dimension retention times e.g. of
biopharmaceuticals by using reference compounds.
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