Theoretical Basis Of 2D-Lc - Agilent Technologies 1290 Infinity II 2D-LC User Manual

14 Theoretical Background

Theoretical basis of 2D-LC

Theoretical basis of 2D-LC

In 2D-LC, fractions from a chromatografic system (first dimension) are
transferred to a second chromatographic separation system (second
dimension). So 2D-LC bases on the application of two independent liquid phase
separation systems to a sample. 2D-LC is mainly used to improve resolution and
sensitivity or to decrease analysis time.
Figure 169 Peak capacity relationship between peak capacities of orthogonal first and second
The most importand benefit of 2D-LC over 1D-LC is the increase of resolving
power, which is especially important if dealing with complex samples.
For an overview on the main differences between 1D- and 2D-LC, refer to the
following topics:
•
•
•
2D-LC User Guide
dimension
"Orthogonality" on page 364,
"Resolution" on page 365, and
"Peak Capacity" on page 368
Peaks not resolved in first
dimension separation
Peaks resolved in second
dimension separation
363