Active Solvent Modulation (Asm); Introduction To Active Solvent Modulation (Asm) - Agilent Technologies 1290 Infinity II 2D-LC User Manual

2 Concepts of 2D-LC

Active Solvent Modulation (ASM)

Active Solvent Modulation (ASM)

Introduction to Active Solvent Modulation (ASM)

In conventional 2D-LC,
dimension column. If the
column, it impairs separation in the second dimension. This results in unretained
elution, broad and distorted peaks, and loss of separation (see
page 36).
Active Solvent Modulation (ASM) dilutes the content of the sampling loop
(sample and
and therefore improves the separation in the second dimension (see
page 36).
Different ASM capillaries allow optimizing the dilution for different applications
(see
The ASM solution is primarily designed for 2D-LC modes multiple heart-cutting
and high-resolution sampling. The 2D-LC Valve ASM is backward compatible to
the standard 2D-LC valve G4236A. If ASM is not needed or for use in
comprehensive 2D-LC, the ASM functionality can be disabled.
Figure 14
2D-LC User Guide
1
D solvent in the sample loop is injected to the second
1
1
D solvent) with weak
"Understanding the ASM Factor"
Schematic representation of the ASM 2D-LC Valve (G4243A) with MHC in countercurrent flow
D solvent has high elution strength in respect to the
2
D solvent before it reaches the
on page 42).
2
D
Figure 16 on
2
D column
Figure 17 on
35