Introduction - Leica BOND-PRIME User Manual

3.1

Introduction

This chapter shows you how to do an example run on the BOND-PRIME Processing Module.
You will create a sample case and configure and process four slides.
The process uses four BOND ready-to-use primary antibodies:
*CD5
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*CD3
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*CD10
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*Bcl-6
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The process uses the default protocol and detection system for these antibodies:
*IHC Protocol F
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BOND-PRIME Polymer DAB Detection System (DS9824)
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The process uses the ancillary BOND-PRIME Hematoxylin (AR0096).
The procedure is also applicable for ISH probes and protocols.
You can interchange the antibody for a probe and replace IHC protocols with ISH protocols.
For details of the following preliminary tasks, refer to the Quick Start chapter in the BOND System User Manual:
Preliminary Checks and Startup*
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Protocol and Reagent Checks
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Setting Up Slides (up to and including labeling slides)
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To improve tissue adhesion, try baking for longer before loading slides.
* related only to the Slide Labeler and BOND System Controller (and Terminal, for BOND-PRIME)
BOND-PRIME User Manual, 91.7500.501 A07
Copyright © 2023 Leica Biosystems Melbourne Pty Ltd
Chapter 3 Quick start
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