The Leica BOND-PRIME manual does not explicitly mention which chemicals cannot be sent down the drain. However, it does state that certain chemicals such as xylene, chloroform, acetone, strong acids (e.g., 20% HCl), and strong alkalis (e.g., 20% NaOH) should not be used on BOND-PRIME Processing Modules. These chemicals may require special disposal methods rather than being sent down the drain.

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Summary of Contents for Leica BOND-PRIME

Page 1 BOND-PRIME F U L L Y A U T O M A T E D I H C A N D I S H S T A I N I N G S Y S T E M USER MANUAL...
Page 2: Table Of Contents
1.13 Connect the processing module and switch on 1.14 Disconnect the processing module 1.15 Move a processing module to a new location 1.16 Decommission and dispose of a processing module BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 3 2.10.2 Load Reagent Trays 2.10.3 Examples of reagent system icons 2.10.4 Examples of Reagent Container icons 2.10.5 View reagent system and Reagent Container details 2.10.6 Unload Reagent Trays BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 4 Clean the Waste Drains and Sump Clean the Pickup Tube and Filter 4.13 Clean the Wash/Prime Stations 4.14 Use the BOND-PRIME ARC Refresh Kit 4.15 Clean the Bulk DI Water Container 4.16 Clean the Locked Bulk Reagent Containers 4.17 Clean the Waste Containers 4.18 Clean the Sump Tray...
Page 5 6.1 System specifications 6.2 Physical specifications 6.3 Electrical power and UPS requirements 6.4 Environmental specifications 6.5 Operating specifications 6.6 Microscope slide specifications 6.7 Transport and storage specifications Index BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 6 Legal notices Trademarks Leica and the Leica logo are registered trademarks of Leica Microsystems IR GmbH and used under license. BOND System, BOND-III, BOND-MAX, BOND-PRIME, BOND-ADVANCE, Covertile, Bond Polymer Refine Detection, Bond Polymer Refine Red Detection, Parallel Automation, Compact Polymer, and Oracle are trademarks of Leica Biosystems Melbourne Pty Ltd ACN 008 582 401.
Page 7 The occurrence of any serious incident(s) that either has led to, or may lead to, death of a patient or user, or the temporary or permanent deterioration in the state of health of a patient or user must be reported to a local Leica Biosystems representative and the relevant local Regulatory Authority.
Page 8 BOND System Controller indefinitely. The images are automatically removed from the BOND Processing Module after one day. Contacting Leica Biosystems For service or support contact your local Leica Biosystems representative or see www.leicabiosystems.com. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 9 Updated Intended Use statement. Various corrections and updates to reflect the latest touchscreen interface and to improve the usability of the manual. Removed Clean the Slide Preparation section. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 10 4.15 Clean the Bulk DI Water Container 4.16 Clean the Locked Bulk Reagent Containers 4.17 Clean the Waste Containers 4.18 Clean the Sump Tray 6 Specifications April 2021 First release. Not released. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 11 To maintain compliance use only the cables supplied with the instrument. WARNING: Any changes or modifications not expressly approved by Leica Biosystems could void the user’s authority to operate this equipment.
Page 12 Class B - This is applicable for all equipment suitable for use in domestic establishments and in establishments directly connected to a low voltage power supply network which supplies buildings used for domestic purposes. Definitions ISM: Industrial, Scientific and Medical RF: Radio Frequency BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 13 This section describes the regulatory and safety symbols used in the product labeling. Regulatory symbols Explanation of the regulatory symbols used for Leica Biosystems products. This glossary provides images of the symbols as presented in the relevant standards, however, some of the symbols may vary in color.
Page 14 In Vitro Diagnostic medical device ISO 15223-1 5.5.1 Indicates a medical device that is intended to be used as an in vitro diagnostic medical device. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 15 Identifies the switch or switch position by means of which part of the equipment is switched on in order to bring it into the standby condition Fuse ISO 60417 5016 Identifies fuse boxes or their location. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 16 An instructional safeguard to keep away from moving parts. Information; general IEC 60417 6222 Identifies the control to examine the status of the equipment, e.g. multifunctional copying machines BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 17 The logo also signifies that the product should be recycled immediately after its environmental protection use period has expired. The date on the label indicates the date of manufacture. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 18 Indicate the acceptable upper and lower limits of relative humidity for transport and storage. This symbol is accompanied by the applicable relative humidity limits. Unconnected port This product has an unconnected port on the syringe pump. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 19 Overhead obstacle. Take care to avoid being struck by or walking into an overhead obstacle. Warning: flammable material ISO 7010 W021 Flammable hazard. Flammable materials may ignite if proper precautions are not followed. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 20 Warnings use symbols with a black border and yellow background. General BOND-PRIME warnings appear below. Other warnings appear in the relevant sections in the manual. Instrument operation To make sure that the BOND-PRIME Processing Module operates correctly, always obey the approved instructions from Leica Biosystems Melbourne Pty Ltd.
Page 21 A malware infection on a BOND-PRIME controller could lead to unexpected behaviors in operation, including disabling processing modules. Take care to ensure your USB storage devices are virus free before connecting them to the BOND-PRIME controller. Further, Leica Biosystems Melbourne Pty Ltd does not pre-install an anti-virus solution;...
Page 22 1.7 Bulk Containers. General cautions Cautions are notifications of hazards that could lead to damage to the BOND equipment or other adverse consequences that do not endanger people. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 23 Cautions use symbols with a black border and white background General BOND-PRIME cautions appear below. Other cautions appear in relevant sections in the manual. Personal Protective Equipment (PPE) You must wear the minimum required PPE before you use reagents, operate, maintain or clean the processing...
Page 24 Bulk reagents that are not compatible can cause unsatisfactory performance and damage to the processing module. Refer to Leica Biosystems Melbourne Pty Ltd for information about compatible bulk reagents. Do not use xylene, chloroform, acetone, strong acids (for example 20% HCl), strong alkalis (for example 20% NaOH) on BOND-PRIME Processing Modules.
Page 25: Bond-Prime Hardware
1.13 Connect the processing module and switch on 1.14 Disconnect the processing module 1.15 Move a processing module to a new location 1.16 Decommission and dispose of a processing module BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 26: About The Bond System
1 BOND-MAX Processing Module 5 Slide Label Printer 2 BOND-PRIME Processing Module 6 BOND System Controller 3 BOND-III Processing Module 7 LIS connection 4 Handheld Barcode scanner BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 27: Bond System Ancillary Materials And Consumable Items
1.1.1 BOND System ancillary materials and consumable items Leica Biosystems Melbourne Pty Ltd supplies the following ancillary materials for use with the BOND-PRIME Processing Module. To get best-quality stained slides and to prevent damage, do not use alternative ancillary materials.
Page 28 BOND-PRIME ARC Refresh Kit: 24 ARC Covertiles 1 Mixing Well Plate Spares BOND-PRIME Suction Cups Required Reagents (not supplied by Leica Biosystems Melbourne Pty Ltd) Reagent-grade alcohol DI water BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 29: The Bond-Prime Processing Module
Chapter 1 BOND-PRIME hardware The BOND-PRIME Processing Module 1.2.1 Front view Legend 2 Touchscreen 1.7 Bulk Containers 1.3 The Hood 1.8 Reservoir Cabinet 1.4 Preload and Unload Drawers 7 Sump Tray 1.6 Reagent Platform BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 30: Rear View
Chapter 1 BOND-PRIME hardware 1.2.2 Rear view Legend 1 Ethernet connection 2 Fuses and power-supply connection BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 31: The Hood
Used to open and close the Hood. 3 Barcode Scanner Used to scan bulk reagent supply bottles and the ARC Refresh Kit. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 32: Preload And Unload Drawers
Preload and Unload Drawers Legend 1 Preload Drawer 2 Unload Drawer See also: 2.5 Preload slides 2.7 Unload slides 4.12 Clean the Slide Drawer Inserts, Waste Drains and Sumps, and Pickup Filter BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 33: Slide Drawer Inserts, Waste Drains And Sumps, And Pickup Filter
1 Preload Slide Drawer Insert 2 Unload Slide Drawer Insert See also: 2.5 Preload slides 2.7 Unload slides 4.12 Clean the Slide Drawer Inserts, Waste Drains and Sumps, and Pickup Filter BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 34: Waste Drains
The Waste Drains are accessible after removal of the Slide Drawer Inserts from the opened drawers. See also: 4.12 Clean the Slide Drawer Inserts, Waste Drains and Sumps, and Pickup Filter BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 35: Sumps And Pickup Tube With Filter
If there is liquid build up in a Sump, it might be because the Pickup Filter is blocked. Refer to 4.12 Clean the Slide Drawer Inserts, Waste Drains and Sumps, and Pickup Filter. See also: 4.12 Clean the Slide Drawer Inserts, Waste Drains and Sumps, and Pickup Filter BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 36: Reagent Platform
See also: 1.10 Reagent Trays 2.10.1 Prepare Reagent Container and Reagent Trays 2.10.2 Load Reagent Trays 2.10.6 Unload Reagent Trays 4.9 Wipe down Reagent Platform and ARC Bank Surfaces BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 37: Bulk Containers
Bulk Waste BOND-PRIME Epitope Retrieval 1 Hazardous Waste WARNING: Use both hands when you lift the DI Water and Bulk/Hazardous Waste containers for cleaning and maintenance tasks. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 38 4.15 Clean the Bulk DI Water Container 4.3 Refill the Alcohol Container 4.16 Clean the Locked Bulk Reagent Containers 4.4 Refill the Lot Tracked Bulk Containers 4.17 Clean the Waste Containers 4.5 Empty the Waste Containers BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 39: Reservoir Cabinet
3 BOND System Wash Working Solution 4 Bulk Waste 5 Hazardous Waste WARNING: Take care not to trip over the Reservoir Cabinet door when it is in the open position. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 40: Ac Power Switch
WARNING: Take care not to trip over the Reservoir Cabinet door when it is in the open position. See also: 1.13 Connect the processing module and switch on 1.14 Disconnect the processing module BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 41: Reagent Trays
Can hold up to 10 reagent containers, although some reagent systems have only 6 containers. You can insert additional reagent containers, for example the ancillary BOND-PRIME Hematoxylin (AR0096), into empty locations. All BOND reagent containers and BOND-PRIME reagent systems must be registered on the BOND System Controller before use. See also: 1.6 Reagent Platform...
Page 42: The Work Surface (Under The Hood)
4.14 Use the BOND-PRIME ARC Refresh Kit 4.7 Start Maintenance 5.3.3 Manually retrieve slides from ARC Modules 4.8 Wipe internal surface of ARC Modules 4.9 Wipe down Reagent Platform and ARC Bank Surfaces 4.13 Clean the Wash/Prime Stations BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 43: Work Surface (Front View)
Bank B (front) numbered 13-24 from left to right Prime Station (for Bulk Reagent Probes) 1.12.5 Wash/Prime Stations 1.12.6 Mixing Well Plate Wash Stations (for Wash Robots) 1.12.7 Slide Preparation Station BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 44: High-Speed Robot
WARNING: If the High-Speed Robot gets stuck in a position above the Work Surface, do not attempt to manually move it. Contact customer support about the problem. See also: 4.10 Clean the Suction Cup 4.11 Replace the Suction Cup BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 45: Probe Selector
The Bulk Reagent Probes are attached to the Probe Selector on the Robot Head. The Probe Selector carousel rotates to position the required probe above an ARC Module. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 46: Wash Robots
If a Wash Robot gets stuck in a position above the Work Surface, do not attempt to manually move it anymore. Contact customer support about the problem BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 47: Arc (Active Reagent Control) Modules
Covertile and Covertile seal, and if necessary, replace the Covertile. ARC Module for debris, and if necessary, clean (Wipe internal surface of ARC Modules on page 129). BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 48 Chapter 1 BOND-PRIME hardware See also: 4.8 Wipe internal surface of ARC Modules 4.14 Use the BOND-PRIME ARC Refresh Kit 5.3.3 Manually retrieve slides from ARC Modules BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 49: Wash/Prime Stations
ARC Probe Wash Station on Bank B (front) is directed to the Hazardous Waste Reservoir both Wash Robot Wash Stations is directed to the Hazardous Waste Reservoir. See also: 4.13 Clean the Wash/Prime Stations BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 50: Mixing Well Plate
The orientation of the Mixing Well Plate on the Mixing Block is not important, however it must be seated correctly within the holder. The processing module will not complete initialization if there is no Mixing Well Plate installed. See also: 4.14 Use the BOND-PRIME ARC Refresh Kit BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 51: Slide Preparation Station
Slide Preparation Station. This is designed to remove unwanted particles, particularly glass particles, from the slide surfaces so that the staining process is not compromised. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 52: Connect The Processing Module And Switch On
Power on the processing module. Open the Reservoir Cabinet door. Turn the AC power switch clockwise. Close the Reservoir Cabinet door. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 53 Log In Screen. This process takes from 8 to 15 minutes. If the processing module fails to initialize, refer to 5.1 Failure to initialize. The Status screen is displayed. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 54: Disconnect The Processing Module
Power down the processing module. Press the standby power button. A pop up window indicates that it is safe to shut down the processing module. Tap Close. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 55 You may need to move the processing module for better access to the rear-panel connectors. Disconnect Ethernet cable from rear of of the processing module. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 56: Move A Processing Module To A New Location
4.18 Clean the Sump Tray. Before you attempt to move a BOND-PRIME Processing Module, use a spanner to rotate the orange gears on the four wheel assemblies. Raise the central feet to allow the processing module to move freely on its wheels.
Page 57 Use only the approved power supply cable. Make sure that you can get access to the wall socket. Evaluate the electromagnetic environment prior to operation of the processing module for interference. CAUTION: Do not operate a BOND-PRIME Processing Module near sources of strong electromagnetic radiation. For example unshielded intentional RF sources, which may interfere with proper operation.
Page 58: Decommission And Dispose Of A Processing Module
(2012/19/EU). In regions outside of the EU, follow local procedures and regulations for the disposal of electronic waste. If you need assistance, contact your local Leica Biosystems representative. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 59: Touchscreen
2.4 Action Queue and Alert Banner 2.5 Preload slides 2.6 Preload Screen 2.7 Unload slides 2.8 Unload Screen 2.9 Detailed case and slide information 2.10 Reagents Screen 2.11 Maintenance Screen BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 60: Log In And Log Out
Reagent Tray onto the Reagent Platform Log in to the touchscreen. On the Log in Screen, tap your user name. Enter your PIN. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 61: Log Out
You can change this time period in the Administration Client on the BOND System Controller. Refer to the BOND System User Manual. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 62: Navigation Bar
Navigation Bar Legend 2.3 Status Screen 2.10 Reagents Screen 2.6 Preload Screen 2.11 Maintenance Screen 2.8 Unload Screen 6 Log in / Log out - refer to 2.1 Log in and log out BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 63: Status Screen
When slides are accepted, they move to the top of the segment and change to blue, earliest first. Slides that are not accepted remain at the bottom of the segment. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 64 Slide accepted. Slides not accepted remain at the bottom of the segment. Also refer to 2.3.1.1 Slides not accepted. Rejected time-critical slide, or a slide with status "Warning". Rejected slide, or a slide with status "Caution". BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 65: Slides Processing Segment
The processing module may not always prioritize loading slides. This is because the processing module automatically performs a basic clean of each ARC Module in between processing slides. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 66 Faulty or disabled ARC Module. Refer to 1.12.4 ARC (Active Reagent Control) Modules The BOND-PRIME Cleaning kit is currently being applied, or a basic clean is being run between slides. ARC Module usage count has been reached. The ARC Module is disabled until the BOND-PRIME Cleaning kit is applied.
Page 67: Slides Processing Complete Segment
Also refer to 2.3.4 View more slide information. Icon Description Slide position available Slide completed Last completed slide in a case Canceled slide or a slide with status "Warning" BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 68: View More Slide Information
Tap on a slide in the segment to view related slide information. Tap on the presented slide image to open the case window and view related case information. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 69: Unexpected Events During Processing
Any events that caused a notification are displayed in Bold text. The system operator or laboratory supervisor should carefully consider the notification events listed, as these provide important details about the nature of the slide notification events. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 70 Done (events noted), ensure that the entire report is inspected. If the status is Done (OK), there is no need to inspect the report. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 71: Action Queue And Alert Banner
To show or hide the Action Queue, tap the Action Queue button. To show more or less information about each item in the Action Queue, tap the arrow to the right of the item. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 72: Complete A Task Suggested In An Action Queue Item
Action Queue Items providing information about a slide will remain in the Action Queue until that slide has finished the run and is removed from the processing module. For maintenance related tasks, refer to 4 Cleaning and maintenance. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 73: Manually Hide The Alert Banner
Tap the X in the banner at the bottom of the screen. Although the BOND-PRIME software allows you to manually hide the Alert Banner, Leica Biosystems Melbourne Pty Ltd recommends that you should, if possible, complete the suggested task.
Page 74: Preload Slides
Bake slides at 60 °C (140 °F) for 60 minutes to improve tissue adhesion. The Preload Drawer is the left-hand drawer. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 75 Slides that are scanned together are scheduled together. Check that all slides are correctly located in the Slide Drawer Insert, and then close the Preload Drawer. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 76: Preload Screen
Loading slides together means they are scanned and then prioritized together.This enables the processing module to complete the last slide as efficiently as possible. Legend 2.6.1 Preload Drawer view 2.6.2 Preload Case view BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 77: Preload Drawer View
Preload Drawer. Slides that are currently being evaluated show no details until the evaluation process is complete. Slide positions on the Preload Drawer view BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 78 Chapter 2 Touchscreen Slide positions in the Preload Drawer See also: 2.5 Preload slides 2.9 Detailed case and slide information BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 79: Preload Case View
Chapter 2 Touchscreen 2.6.2 Preload Case view Slide positions on the Preload Case view Icon Description Detected slide Unreadable slide Identified slide Accepted slide Slide with status "Caution" BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 80 Slide with status “Warning” Slides in this case are either accepted or evaluating Slides in this case are in progress See also: 2.5 Preload slides 2.9 Detailed case and slide information BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 81: Unload Slides
The Unload Drawer is the right-hand drawer. Check that the Unload Drawer status LED is green, and then pull the handle to open the drawer. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 82 "Unexpected slide" warning. If you do not unload all the slides, they will no longer be hydrated and staining quality could be compromised. Close the Unload Drawer. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 83: Unload Screen
Unload case view—displays a physical representation of the location of each slide in each case in the Unload Drawer. A toggle at the top of the screen allows you to switch views. Legend 2.8.1 Unload Drawer view 2.8.2 Unload case view BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 84: Unload Drawer View
The Unload Drawer view shows the relationship between the slide positions shown on-screen and their actual positions in the Unload Drawer. Slide positions on the Unload Drawer view Slide positions in the Unload Drawer BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 85: Unload Case View
See also: 2.7 Unload slides 2.9 Detailed case and slide information 2.3.5 Unexpected events during processing (slides with status "Caution") 2.8.2 Unload case view Slide positions on the Unload Case view BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 86 All slides in this case have successfully completed processing Unexpected slide - refer to 2.7 Unload slides See also: 2.7 Unload slides 2.9 Detailed case and slide information 2.3.5 Unexpected events during processing (slides with status "Caution") BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 87: Detailed Case And Slide Information
Preload or Unload screen (case view) - the first slide in the case is highlighted in the information window that appears BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 88 Chapter 2 Touchscreen Tap a slide in the information window to view its details. See also: 2.9.1 View each slide in a case 2.9.2 Stop a slide in progress BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 89: View Each Slide In A Case
Chapter 2 Touchscreen 2.9.1 View each slide in a case Tap a slide to display the details of that slide. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 90: Stop A Slide In Progress
On the Case Details screen, select the slide, and then tap Stop. Tap Yes. Tap Close. A canceled slide will be transferred to the Unload Drawer and will be shown in red. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 91: Reagents Screen
WARNING: You must wear the minimum required Personal Protective Equipment (PPE) before you use reagents or operate the processing module. Refer to General cautions. Single Reagent Tray BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 92 Make sure the top barcode labels are fully adhered to the containers – press down any labels that are lifting. Wipe any moisture/condensation from the top barcode label. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 93: Load Reagent Trays
If a reagent is not registering after a few minutes, remove the Reagent Tray and re-insert it into a different Reagent Lane, to re-trigger the reagent scans. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 94: Examples Of Reagent System Icons
2.10.4 Examples of Reagent Container icons Vacant Reagent Tray position Unallocated Reagent Container Allocated Reagent Container Unrecognized Reagent Container Invalid or expired Reagent Container (or reagent system) Reagent not registered BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 95: View Reagent System And Reagent Container Details
Chapter 2 Touchscreen 2.10.5 View reagent system and Reagent Container details Reagent System Details BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 97: Unload Reagent Trays
Close the Reagent Container lids firmly to prevent reagent evaporation. You will hear a click when you fully close the lid. Immediately store the reagents as recommended on the label or reagent data sheet. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 98: Maintenance Screen
5 Replace Suction Cup 4.6 Use the BOND-PRIME Cleaning Kit 4.11 Replace the Suction Cup 3 Start maintenance 6 Clean Bulk Reagent Containers 4.7 Start Maintenance 4.16 Clean the Locked Bulk Reagent Containers BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 99: Quick Start
In this section: 3.1 Introduction 3.2 Start the processing module 3.3 Load the Reagent Tray and DS9824 Detection System 3.4 Preload, process, and unload the slides BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 100: Introduction
Chapter 3 Quick start Introduction This chapter shows you how to do an example run on the BOND-PRIME Processing Module. You will create a sample case and configure and process four slides. The process uses four BOND ready-to-use primary antibodies:...
Page 101: Start The Processing Module
Log In Screen. This process takes from 8 to 15 minutes. If the processing module fails to initialize, refer to 5.1 Failure to initialize. The Status screen is displayed. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 102 Warning: Take immediate action. Caution: Take action at your next opportunity. Information: For your awareness. If the processing module does not initialize, refer to 5.1 Failure to initialize. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 103: Load The Reagent Tray And Ds9824 Detection System
DAB Part B x 2 Load both Reagent Trays onto the processing module. Place the Reagent Tray in the Reagent Platform. Push the Reagent Tray until you hear a click. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 105: Preload, Process, And Unload The Slides
Slides that are scanned together are scheduled together. Check that all slides are correctly located in the Slide Drawer Insert, and then close the Preload Drawer. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 106 When slides are being processed on the Work Surface, they are displayed in the Processing area of the screen. When slides are in the Unload Drawer, they are displayed in the Complete area of the screen. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 107 Drawer reaches capacity, it can hinder processing, extending the Estimated Time of Completion. When you close the Unload Drawer, it must not contain any slides. Close the Unload Drawer. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 108 If there are, unload the reagents and then close Reagent Container lids firmly to prevent reagent evaporation. Immediately store the reagents as recommended on the label or reagent data sheet. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 109: Cleaning And Maintenance
4.11 Replace the Suction Cup 4.12 Clean the Slide Drawer Inserts, Waste Drains and Sumps, and Pickup Filter 4.13 Clean the Wash/Prime Stations 4.14 Use the BOND-PRIME ARC Refresh Kit 4.15 Clean the Bulk DI Water Container 4.16 Clean the Locked Bulk Reagent Containers 4.17 Clean the Waste Containers...
Page 110: Maintenance Schedules
Preventative service reminder When you use the BOND-PRIME Processing Module, look for leaks and worn or damaged parts. This user manual contains instructions that tell you how to clean or replace some parts. Notify customer support if it is necessary to repair or replace other parts.
Page 111 Clean Barcode Scanner (on Hood) using a lint-free cloth moistened with DI water Four-monthly/5000 slides Use the BOND-PRIME ARC Refresh Kit (replace Mixing Well Plate and ARC Covertiles) Six-monthly (or if the processing module is idle for more than 14 days)
Page 112: Cleaning And Maintenance Checklist
(on BOND System Controller) NOTIFICATION Use the BOND-PRIME Cleaning Kit * If it is necessary, do these tasks more frequently. For week starting ___________________to ____________________ For month of _____________________ BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 113: Refill The Di Water Container
WARNING: Use both hands when you lift the DI Water Container. Return the container quickly to ensure there is DI Water available. Remove the DI Water Container cap. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 114 Listen for a click sound to confirm the container is locked in place. Make sure the container is fully inserted. Failure to do so can cause slides to be rejected in the Preload Drawer. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 115: Refill The Alcohol Container
Before you start this procedure, ensure you are logged in to the processing module. Refer to 2.1 Log in and log out. Tap Maintenance. Tap Unlock next to Refill alcohol. A popup window is displayed prompting you to refill the Bulk Alcohol Container. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 116 30 seconds. Pull the top of the container towards you. Open the container lid. Pour the alcohol into the bulk container up to the maximum fill line. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 117 Chapter 4 Cleaning and maintenance Close the container lid. Push the container into the processing module. Tap Close. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 118: Refill The Lot Tracked Bulk Containers
Before you start this procedure, ensure you are logged in to the processing module. Refer to 2.1 Log in and log out. BOND-PRIME has the following Lot Tracked Bulk Containers: Dewax Solution Wash Concentrate Scan the barcode on the supply bottle to ensure lot number tracking occurs.
Page 119 30 seconds. Pull the top of the container towards you. Open the container lid. Open the bottle and dispose of the tamper-proof seal ring according to laboratory procedures. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 120 WARNING: To avoid spillage, DO NOT refill Bulk Reagent Containers while they are away from the processing module. Close the container lid. Push the caddy back into the processing module. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 121: Empty The Waste Containers
Press the Waste button. Pull the container out of the processing module. WARNING: Use both hands when you lift the Waste containers. Remove the Waste Container cap. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 122 Listen for a click sound to confirm the container is locked in place. Make sure the container is fully inserted. Failure to do so can cause slides to be rejected in the Preload Drawer. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 123: Use The Bond-Prime Cleaning Kit
BOND-PRIME Cleaning Kit. ARC Modules must be cleaned when the usage count is between 17 and 23. You must register the BOND-PRIME Cleaning Kit on the BOND System Controller (refer to BOND System User Manual). Some ARC Modules are disabled until cleaning is completed.
Page 124 Push the Reagent Tray into the Reagent Platform. Listen for a click to confirm the tray is correctly inserted. The BOND-PRIME Cleaning Kit is displayed on the Reagent screen. Tap Schedule next to Run BOND-PRIME Cleaning Kit. BOND-PRIME User Manual, 91.7500.501 A07...
Page 125 The time in minutes for the cleaning process to finish is displayed. When the BOND-PRIME Cleaning Kit is completed, the Schedule button is enabled and the number of slides that can be processed before the kit is needed again is displayed.
Page 126 Chapter 4 Cleaning and maintenance Close the Reagent Container Lid. You will hear a click when the lid is secured. Store Reagent Containers between 2°C - 8°C. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 127: Start Maintenance
Use the Start maintenance procedure to access the Work Surface, clean the Bulk Reagent Containers, replace the Suction Cup, or use the BOND-PRIME ARC Refresh Kit. Before you start this procedure, ensure you are logged in to the processing module. Refer to 2.1 Log...
Page 128 When maintenance is started, the processing module automatically opens all ARC Modules. When the Hood is unlocked, the Hood Lock Status on the Maintenance screen changes to Unlocked. Open the Hood. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 129: Wipe Internal Surface Of Arc Modules
Check the Status screen to ensure there are: no slides currently being processed (Processing) no slides in the Preload Drawer (Loaded) and Unload Drawer (Complete). Refer to 2.3 Status Screen. Tap Maintenance. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 130 ARC Modules. When the Hood is unlocked, the Hood Lock Status on the Maintenance screen changes to Unlocked. Moisten a clean lint-free cloth with 70% ethanol solution. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 131 Covertile Seal. If you no longer require access to the Work Surface, close the Hood and then continue with this procedure. Tap Stop maintenance. Tap Yes. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 132 There is a wait time while the processing module is preparing to return to clinical operation. The processing module automatically closes the ARC Modules and locks the Hood when you stop maintenance. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 133: Wipe Down Reagent Platform And Arc Bank Surfaces
Check the Status screen to ensure there are: no slides currently being processed (Processing) no slides in the Preload Drawer (Loaded) and Unload Drawer (Complete). Refer to 2.3 Status Screen. Tap Maintenance. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 134 ARC Modules. When the Hood is unlocked, the Hood Lock Status on the Maintenance screen changes to Unlocked. Remove all reagent trays from the Reagent Platform. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 135 Wipe both Wash Robot rails. If you no longer require access to the Work Surface, close the Hood and then continue with this procedure. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 136 There is a wait time while the processing module is preparing to return to clinical operation. The processing module automatically closes the ARC Modules and locks the Hood when you stop maintenance. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 137: Clean The Suction Cup
Check the Status screen to ensure there are: no slides currently being processed (Processing) no slides in the Preload Drawer (Loaded) and Unload Drawer (Complete). Refer to 2.3 Status Screen. Tap Maintenance. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 138 Locate the Suction Cup on the Robot Head. CAUTION: To help prevent dropping the Suction Cup into the processing module, carefully move the High-Speed Robot over the Reagent Platform. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 139 Flex the Suction Cup to remove residual wax, then clean with the lint-free cloth until no wax residue remains. Ensure the small hole is clear of any blockages. Refit the Suction Cup onto the Robot Head. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 140 There is a wait time while the processing module is preparing to return to clinical operation. The processing module automatically closes the ARC Modules and locks the Hood when you stop maintenance. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 141: Replace The Suction Cup
Check the Status screen to ensure there are: no slides currently being processed (Processing) no slides in the Preload Drawer (Loaded) and Unload Drawer (Complete). Refer to 2.3 Status Screen. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 142 When maintenance is started, the processing module automatically opens all ARC Modules. When the Hood is unlocked, the Hood Lock Status on the Maintenance screen changes to Unlocked. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 143 Install a new Suction Cup. Remove the Suction Cup from its packaging. Attach the Suction Cup to the Robot Head. Tap Done next to Replace Suction Cup. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 144 There is a wait time while the processing module is preparing to return to clinical operation. The processing module automatically closes the ARC Modules and locks the Hood when you stop maintenance. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 145: Clean The Slide Drawer Inserts, Waste Drains And Sumps, And Pickup Filter
Check the Status screen to ensure there are: no slides currently being processed (Processing) no slides in the Preload Drawer (Loaded) and Unload Drawer (Complete). Refer to 2.3 Status Screen. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 146 When maintenance is started, the processing module automatically opens all ARC Modules. When the Hood is unlocked, the Hood Lock Status on the Maintenance screen changes to Unlocked. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 147 Use the finger tab on the front fascia to assist in lifting the insert out of the drawer. Remove the Slide Drawer Insert from the Preload Drawer. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 148 Remove the Slide Drawer Insert from the Unload Drawer. Wash inserts with warm soapy water. Rinse well with running water. Allow to dry completely before re-installation in the same processing module. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 149: Clean The Waste Drains And Sump
Wipe the Sumps with the lint-free cloth. The Sumps are located under and behind the Preload and Unload Drawers. They are accessible via the Work Surface after fully opening the drawers. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 150: Clean The Pickup Tube And Filter
Clean the Pickup Tube and Filter Sumps Unload Drawer Pickup Tube Pickup Filter Rotate the Pickup Tube with Filter at elbow. Press the orange sleeve to remove the Pickup Tube and Filter. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 151 Flush Pickup Tube and Filter using clean water if required. Confirm the Filter is free of debris. Refit the Pickup Tube with Filter into the orange sleeve, making sure you push it in all the way. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 153 There is a wait time while the processing module is preparing to return to clinical operation. The processing module automatically closes the ARC Modules and locks the Hood when you stop maintenance. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 154: Clean The Wash/Prime Stations
Check the Status screen to ensure there are: no slides currently being processed (Processing) no slides in the Preload Drawer (Loaded) and Unload Drawer (Complete). Refer to 2.3 Status Screen. Tap Maintenance. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 155 When maintenance is started, the processing module automatically opens all ARC Modules. When the Hood is unlocked, the Hood Lock Status on the Maintenance screen changes to Unlocked. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 156 Clean only the top surfaces of Wash/Prime Stations with lint-free cloth. CAUTION: Do not use cotton buds. 1 Bulk Probe Prime Station 2 Wash Robot Wash Stations 3 ARC Probe Wash Stations BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 157 There is a wait time while the processing module is preparing to return to clinical operation. The processing module automatically closes the ARC Modules and locks the Hood when you stop maintenance. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 158: Use The Bond-Prime Arc Refresh Kit
Chapter 4 Cleaning and maintenance 4.14 Use the BOND-PRIME ARC Refresh Kit The BOND-PRIME ARC Refresh Kit contains replacement: Covertiles Mixing Well Plate. Use the ARC Refresh Kit every 5000 slides or 4 months, whichever comes first. Each IHC single stain counts as one usage for Covertile life purposes.
Page 159 There is a wait time after tapping Yes while the processing module is preparing the Work Surface for maintenance and unlocking the Hood. When maintenance is started, the processing module automatically opens all ARC Modules. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 160 Lift up the Mixing Well Plate. Dispose of the Mixing Well Plate according to laboratory procedures. WARNING: Take care to prevent liquid spillage when removing and disposing of the Mixing Well Plate. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 161 Attach the Covertile Hook to the rear of the ARC Module. Gently push the Covertile Thumbnail until the Covertile is in place in the ARC Module. Push the ARC Module Latch up. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 162 Chapter 4 Cleaning and maintenance Close the Hood. Tap Stop maintenance. Tap Yes. The processing module automatically closes the ARC Modules when you stop maintenance. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 163: Clean The Bulk Di Water Container
WARNING: Use both hands when you lift the DI Water Container. Return the container quickly to ensure there is DI Water available. Remove the DI Water Container cap. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 164 Put the lid back on the container and briskly shake the container. Empty the contents according to your laboratory procedure. Refill the DI Water Container and then put the DI Water Container cap back on. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 165 Listen for a click sound to confirm the container is locked in place. Make sure the container is fully inserted. Failure to do so can cause slides to be rejected in the Preload Drawer. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 166: Clean The Locked Bulk Reagent Containers
Check the Status screen to ensure there are: no slides currently being processed (Processing) no slides in the Preload Drawer (Loaded) and Unload Drawer (Complete). Refer to 2.3 Status Screen. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 167 When maintenance is started, the processing module automatically opens all ARC Modules. When the Hood is unlocked, the Hood Lock Status on the Maintenance screen changes to Unlocked. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 168 The two locked containers on the left are the Alcohol and Dewax Solution Containers. Clean and then reinstall one container at a time, as the containers cannot stand upright on a flat surface. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 169 Slide the container up and out of the caddy. WARNING: To avoid spillage, DO NOT refill Bulk Reagent Containers while they are away from the processing module. Empty the container into a hazardous waste drum. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 170 Empty the container when completed. Close the lid and return the container to its caddy. Push the container down until you hear a click. Close the caddy. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 171 If you do not need to clean any other Reagent Containers, Stop maintenance. For the ER1, ER2 and BOND-PRIME Wash Solution Concentrate Containers The 3 containers on the right are the ER1, ER2, and BOND- PRIME Wash Solution Concentrate Containers.
Page 172 Empty the container according to laboratory procedures Rinse the container: Thoroughly rinse the container with DI Water. Gently shake the container. Empty the container according to laboratory procedures BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 173 Close the lid and return the container to its caddy. Do not refill the container yet. Push the container down until you hear a click. Close the caddy. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 174 Locked Reagent Containers. Refill the Bulk Reagent Containers Refill the containers: 4.3 Refill the Alcohol Container 4.4 Refill the Lot Tracked Bulk Containers Stop maintenance Tap Stop maintenance. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 175 There is a wait time while the processing module is preparing to return to clinical operation. The processing module automatically closes the ARC Modules and locks the Hood when you stop maintenance. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 176: Clean The Waste Containers
Press the Waste button. Pull the container out of the processing module. WARNING: Use both hands when you lift the Bulk/Hazardous Waste containers. Remove the Waste Container cap. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 177 Put the lid back on the container and briskly shake the container. Empty the contents according to your laboratory procedure. Put the Waste Container cap back on. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 178 Listen for a click sound to confirm the container is locked in place. Make sure the container is fully inserted. Failure to do so can cause slides to be rejected in the Preload Drawer. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 179: Clean The Sump Tray
Always treat the waste from the Sump Tray as hazardous. If there is an excessive amount of waste in the Sump Tray, contact Leica Biosystems Support. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 180 Chapter 4 Cleaning and maintenance Moisten a clean lint-free cloth with 70% ethanol solution. Wipe the Sump Tray with the lint-free cloth. Refit the Sump Tray in the processing module. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 181: Stop Maintenance
Hood is down Before you start this procedure, ensure you are logged in to the processing module. Refer to 2.1 Log in and log out. Tap Maintenance. Tap Stop maintenance. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 182 There is a wait time while the processing module is preparing to return to clinical operation. The processing module automatically closes the ARC Modules and locks the Hood when you stop maintenance. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 183: Shutdown The Processing Module
Power down the processing module. Press the standby power button. A pop up window indicates that it is safe to shut down the processing module. Tap Close. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 184 Turn the AC power switch counterclockwise. Close the Reservoir Cabinet door. WARNING: Take care not to trip over the Reservoir Cabinet door when it is in the open position. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 185: Replace Power Supply Fuses
Turn the AC power switch counterclockwise. Close the Reservoir Cabinet door. WARNING: Take care not to trip over the Reservoir Cabinet door when it is in the open position. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 186 Plug the mains power cable into the rear of the processing module. Plug the mains power cable into the wall socket. You may need to move the processing module for better access to the rear-panel connectors. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 187 Log In Screen. This process takes from 8 to 15 minutes. If the processing module fails to initialize, refer to 5.1 Failure to initialize. The Status screen is displayed. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 188: Troubleshooting
In this section: 5.1 Failure to initialize 5.2 Network connectivity error 5.3 Manually retrieve slides from the processing module 5.4 Remove a slide fragment from an ARC Module BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 189: Failure To Initialize
The BOND System Controller must be up and running before connecting the processing modules. Restart the processing module. If the network connectivity error is displayed again, contact your local Leica Biosystems representative. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 190: Manually Retrieve Slides From The Processing Module
WARNING: You must wear the minimum required PPE before you maintain the processing module. Refer to General cautions. If prompted by a message in the Action Queue to remove slides from the Preload or Unload Drawer, follow this procedure. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 191: Manually Retrieve Slides From The Work Surface
Check the drawers for debris. If there is debris, clean the drawers. Refer to 4.12 Clean the Slide Drawer Inserts, Waste Drains and Sumps, and Pickup Filter. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 192 Open the Hood and retrieve the slides. Check the Work Surface for debris. If there is debris, clean the Work Surface. Refer to 4.9 Wipe down Reagent Platform and ARC Bank Surfaces. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 193: Manually Retrieve Slides From Arc Modules
Before you start this procedure, ensure you are logged in to the processing module. Refer to 2.1 Log in and log out. Press the standby power button. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 194 Initialization will fail, but the ARC Modules with slides detected are automatically opened. Power off the processing module again. Turn the AC power switch counterclockwise. Close the Reservoir Cabinet door. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 195 This is done automatically when you start the processing module. If you no longer require access to the Work Surface, close the Hood and then continue with this procedure. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 196: Manually Retrieve Slides From Arc Modules During A Power Failure
Turn the AC power switch counterclockwise. Close the Reservoir Cabinet door. WARNING: Take care not to trip over the Reservoir Cabinet door when it is in the open position. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 197 CAUTION: Be careful not to drop slides when manually handling them over the Work Surface. CAUTION: Do not close the ARC Modules manually. This is done automatically when you start the processing module. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 198: Remove A Slide Fragment From An Arc Module
If not, then the slide breakage has not been detected. Open the Preload Drawer and remove the slides, then wait until all slides have finished processing. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 199 (Processing). Refer to 2.3 Status Screen. Check each ARC Module for glass fragments and manually remove the fragments according to 5.3.3 Manually retrieve slides from ARC Modules. BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 200: Specifications
6.1 System specifications 6.2 Physical specifications 6.3 Electrical power and UPS requirements 6.4 Environmental specifications 6.5 Operating specifications 6.6 Microscope slide specifications 6.7 Transport and storage specifications BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 201: System Specifications
BOND-ADVANCE 8- or 16-port Ethernet switches (for a maximum of 30 processing modules, when switches are connected together) * * any combination of processing modules: BOND-PRIME , BOND-III , BOND-MAX Leica Biosystems Melbourne Pty Ltd must supply the BOND Device specifications System , BOND-ADVANCE Controllers, and BOND-ADVANCE Terminals.
Page 202: Environmental Specifications
BOND-PRIME Epitope Retrieval Solution 1 1.25 L BOND-PRIME Epitope Retrieval Solution 2 1.25 L BOND-PRIME Wash Solution Concentrate 1.25 L BOND-PRIME Wash Working Solution Reservoir 1 L Bulk DI Water capacity Container 4.5 L Reservoir 5 L BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 203: Microscope Slide Specifications
Maximum 2 stacked labels Skew angle User to place the label on straight. Usable slide label area (within top frosted surface) No label overhang permitted. and permitted slide label placement BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 204: Transport And Storage Specifications
(236.2 in x 157.4 in) to uncrate Movement on casters Minimum door width of 850 mm (33.5 in) Traverse a maximum ramp angle of 7 degrees Shipping methods Road, air and sea freight compatible BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 205: Index
Bulk Waste Container clean clean remove slide empty remove slide during power failure ARC Probe ARC Probe Wash Stations case information, view clean case properties cautions 22, 71 BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 206 Bulk Reagent Containers electrical specifications Connect processing module empty Waste Container Consumables environmental specifications contact Leica Biosystems Ethernet Containers events, view Bulk Reagent Reagent copyright Covertile replace BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 207 93, 103 Alert Banner load slides High-Speed Robot Locked Reagent Containers ARC Probe refill Bulk Reagent Probes log in 60, 101 ID Imager Probe Selector log out BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 209 60, 101 regulatory symbols sign out relocate processing module Single Reagent Tray 36, 41 remove slide baking Reagent Trays Slide Drawer Inserts slides clean slides from ARC Modules BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 210 Touchscreen start trademarks maintenance transport specifications processing module Trays Status Screen load Reagent Trays stop Reagent 36, 41 maintenance Slide Drawers stop maintenance unload Reagents Trays BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...
Page 211 Work Surface reagent container details access 98, 127, 181, 183 reagent system details retrieve slide rear slide information warnings 20, 71 wash ARC Modules Suction Cup BOND-PRIME User Manual, 91.7500.501 A07 Copyright © 2023 Leica Biosystems Melbourne Pty Ltd...

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