Page 1 Agilent InfinityLab LC Series Fluorescence Detectors User Manual...
Page 2: Warranty
Copyright adhered to, could result in damage manual and any information contained herein, including but not limited to the © Agilent Technologies, Inc. to the product or loss of important implied warranties of merchantability 2016-2024 data. Do not proceed beyond a and fitness for a particular purpose.
Page 3: Table Of Contents
Contents In This Book 6 Introduction 7 Overview of the Module 8 Product Description of the 1260 Infinity III Fluorescence Detector (G7121A) 9 Features of the 1260 Infinity III Fluorescence Detector (G7121A) 10 Product Description of the 1260 Infinity III Fluorescence Detector Spectra (G7121B) 11 Features of the 1260 Infinity III Fluorescence Detector Spectra (G7121B) 12 Operating Principle 13 Site Requirements and Specifications 28...
Page 4 Optimization Overview 105 How to Collect Spectra with Modes SPECTRA ALL IN PEAK and APEX SPECTRA ONLY 107 Design Features Help Optimization 110 Finding the Best Wavelengths 111 Finding the Best Signal Amplification 113 Changing the Xenon Flash Lamp Frequency 119 Selecting the Best Response Time 121 Reducing Stray Light 123 Diagnostics and Troubleshooting 125 Diagnostic Features 127...
Page 5 USB 241 Hardware Information 242 General Hardware Information 243 Module-Specific Hardware Information 256 Appendix 259 General Safety Information 260 Material Information 268 At-a-Glance Details About Agilent Capillaries 276 Waste Electrical and Electronic Equipment (WEEE) Directive 280 Radio Interference 281 Sound Emission 282 Agilent Technologies on Internet 283 Fluorescence Detectors User Manual...
Page 6: In This Book
3583439627 ag-textnode-base-id: 3583439627 In This Book This manual covers the following Agilent InfinityLab LC Series modules: • Agilent 1260 Infinity III Fluorescence Detector (G7121A) • Agilent 1260 Infinity III Fluorescence Detector Spectra (G7121B) Fluorescence Detectors User Manual...
Page 7: Introduction
ag-textnode-version-id: 9007205351931147 ag-textnode-base-id: 6097190155 Introduction This chapter gives an introduction to the module and instrument overview. Overview of the Module 8 Product Description of the 1260 Infinity III Fluorescence Detector (G7121A) 9 Features of the 1260 Infinity III Fluorescence Detector (G7121A) 10 Product Description of the 1260 Infinity III Fluorescence Detector Spectra (G7121B) 11 Features of the 1260 Infinity III Fluorescence Detector Spectra (G7121B) 12 Operating Principle 13...
Page 8: Overview Of The Module
Introduction Overview of the Module ag-textnode-version-id: 9007202841584779 ag-textnode-base-id: 3586843787 Overview of the Module Table 1: Detector versions Version Description G7121A Introduced as 1260 Infinity III FLD without spectra and multi-signal capabilities. Maximum data rate is 74 Hz. G7121B SPECTRA Introduced as 1260 Infinity III FLD with spectra and multi-signal capabilities.
Page 9: Product Description Of The 1260 Infinity Iii Fluorescence Detector (G7121A)
Introduction Product Description of the 1260 Infinity III Fluorescence Detector (G7121A) ag-textnode-version-id: 9007202841589259 ag-textnode-base-id: 3586848267 Product Description of the 1260 Infinity III Fluorescence Detector (G7121A) The proven optical and electronic design of the Agilent 1260 Infinity III Fluorescence Detector provides highest sensitivity for the analysis of trace-level components.
Page 10: Features Of The 1260 Infinity Iii Fluorescence Detector (G7121A)
Introduction Features of the 1260 Infinity III Fluorescence Detector (G7121A) ag-textnode-version-id: 3586852747 ag-textnode-base-id: 3586852747 Features of the 1260 Infinity III Fluorescence Detector (G7121A) • Lowest limits of detection with a Raman S/N > 3000 (using dark signal noise reference). Simplified optical design for optimized baseline stability. •...
Page 11: Product Description Of The 1260 Infinity Iii Fluorescence Detector Spectra (G7121B)
Introduction Product Description of the 1260 Infinity III Fluorescence Detector Spectra (G7121B) ag-textnode-version-id: 9007202841598731 ag-textnode-base-id: 3586857739 Product Description of the 1260 Infinity III Fluorescence Detector Spectra (G7121B) The Agilent 1260 Infinity III Fluorescence Detector Spectra brings high-sensitivity fluorescence detection to your laboratory. This easy-to-use detector provides quantitative data and fluorescence spectra from a single run.
Page 12: Features Of The 1260 Infinity Iii Fluorescence Detector Spectra (G7121B)
Introduction Features of the 1260 Infinity III Fluorescence Detector Spectra (G7121B) ag-textnode-version-id: 3586862219 ag-textnode-base-id: 3586862219 Features of the 1260 Infinity III Fluorescence Detector Spectra (G7121B) • Rotating gratings for multi-signal and online spectral data acquisition without loss in sensitivity. • Lowest limits of detection with a Raman S/N > 3000 (using dark signal noise reference).
Page 13: Operating Principle
Introduction Operating Principle ag-textnode-version-id: 3982679691 ag-textnode-base-id: 3982679691 Operating Principle ag-textnode-version-id: 3586867211 ag-textnode-base-id: 3586867211 How the Detector Operates Luminescence Detection Luminescence, the emission of light, occurs when molecules change from an excited state to their ground state. Molecules can be excited by different forms of energy, each with its own excitation process.
Page 14 Introduction Operating Principle rotational sub-levels of its ground state, emitting light, see Figure 4 on page 14. The characteristic maxima of absorption for a substance is its λ , and for emission its λ absorption emission λ S₁ radiationless transition S₀ Figure 4: Relationship of Excitation and Emission Wavelengths Photoluminescence is the collective name for two phenomena, fluorescence and phosphorescence, which differ from each other in one characteristic way —...
Page 15: Raman Effect
Introduction Operating Principle spin change S₁ T₁ Phosphorescence S₀ Figure 5: Phosphorescence Energy Transitions The molecule must change its spin back again before it can return to its ground state. Since the chance of colliding with another molecule with the necessary spin for change is slight, the molecule remains in its triplet state for some time.
Page 16: Optical Unit
Introduction Operating Principle Raman Scatter Raleigh Scatter (new wavelength) (same wavelength as incident light) Scattered Light Incident Light Sample Figure 6: Raman The energy difference between the incident light (E ) and the Raman scattered light (E ) is equal to the energy involved in changing the molecule's vibrational state (i.e.
Page 17 Introduction Operating Principle Flash lamp board Trigger Pack EM Grating assembly Xenon Flash lamp Slit EM Condenser lens EX Cutoff filter Slit EX Photo Multiplier Tube Mirror Condenser lens EX Grating assembly REF Diode Diffuser Flow Cell Figure 7: Optical Unit The radiation source is a xenon flash-lamp.
Page 18 Introduction Operating Principle Relative Intensity Wavelenght [nm] Figure 8: Lamp Energy Distribution (vendor data) The radiation emitted by the lamp is dispersed and reflected by the excitation monochromator grating onto the cell entrance slit. The holographic concave grating is the main part of the monochromator, dispersing and reflecting the incident light.
Page 19 Introduction Operating Principle The geometry of the grooves is optimized to reflect almost all of the incident light, in the 1 order and disperse it with about 70 % efficiency in the ultra-violet range. Most of the remaining 30 % of the light is reflected at zero order, with no dispersion.
Page 20 Introduction Operating Principle The excitation and emission gratings are similar in design, but have different blaze wavelengths. The excitation grating reflects most 1 order light in the ultra- violet range around 250 nm, whereas the emission grating reflects better in the visible range around 400 nm.
Page 21 Introduction Operating Principle The selected wavelength of light is reflected onto the slit in the wall of the photo- multiplier compartment of the optical unit. The bandwidth of the emitted light is 20 nm. On the photocathode, Figure 12 on page 21, incident photons generate electrons.
Page 22: Reference System
Introduction Operating Principle ag-textnode-version-id: 3586876171 ag-textnode-base-id: 3586876171 Reference System A reference diode, located behind the flow cell, measures the excitation (EX) light transmitted by the flow cell and corrects flash lamp fluctuations and long-term intensity drift. Because of a non-linear output of the diode (depending on the EX- wavelength), the measured data are normalized.
Page 23: Phosphorescence Detection
Introduction Operating Principle Intensity Track and Hold Ignite Time [µsec] Figure 13: Measurement of Fluorescence ag-textnode-version-id: 3586890123 ag-textnode-base-id: 3586890123 Phosphorescence Detection An appropriate parameter set will be specified as soon as you chose the phosphorescence detection mode (special setpoints under FLD parameter settings).
Page 24: Processing Of Raw Data
Introduction Operating Principle ag-textnode-version-id: 3586894603 ag-textnode-base-id: 3586894603 Processing of Raw Data If the lamp flashes at single wavelength and high-power, then the fluorescence data rate is 296 Hz. That means that your sample is illuminated 296 times per second, and any luminescence generated by the components eluted from the column is measured 296 times per second.
Page 25 Introduction Operating Principle PMTGAIN Phosphorescence Fluorescense Figure 16: PMTGAIN: Amplification of Signal Check proposed PMTGAIN. Deviations of more than 2 PMT gains should be corrected in the method. Each PMTGAIN step is increased approximately by a factor of 2 (range 0 - 18). To optimize your amplification for the peak with the highest emission, raise the PMTGAIN setting until the best signal-to-noise is achieved.
Page 26 Introduction Operating Principle Small S/N ratio Bunched data points RESPONSETIME=125 Boxcar gives 3 points filter per boxcar Filtered data points High S/N rato Figure 17: RESPONSETIME: Signal-to-Noise Ratio Fluorescence Detectors User Manual...
Page 27: System Overview
Introduction Operating Principle ag-textnode-version-id: 3586839307 ag-textnode-base-id: 3586839307 System Overview EX slit Mirror Emission grating EM slit PMT slit Xenon flash Source lamp lens Photo Excitation Emission lens multiplier grating Flow cell Reference diode Diffuser Detector (FLD) Safety cap Figure 18: Optical path of the FLD Fluorescence Detectors User Manual...
Page 28: Site Requirements And Specifications
ag-textnode-version-id: 9007203237517323 ag-textnode-base-id: 3982776331 Site Requirements and Specifications This chapter provides information on environmental requirements, physical and performance specifications. Site Requirements 29 Power Considerations 29 Power Cords 30 Bench Space 31 Condensation 31 Specifications of the 1260 Infinity III Fluorescence Detector (G7121A) 32 Specifications of the 1260 Infinity III Fluorescence Detector Spectra (G7121B) 35 Specification Conditions 38 Fluorescence Detectors User Manual...
Page 29: Site Requirements
Site Requirements and Specifications Site Requirements ag-textnode-version-id: 3584373771 ag-textnode-base-id: 3584373771 Site Requirements A suitable environment is important to ensure optimal performance of the instrument. ag-textnode-version-id: 3602103563 ag-textnode-base-id: 3602103563 Power Considerations The module power supply has wide ranging capability. It accepts any line voltage in the range described in Physical Specifications.
Page 30: Power Cords
— Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment. — Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
Page 31: Bench Space
Site Requirements and Specifications Site Requirements Electrical shock hazard WARNING ag-struc-frag-safety-version-id: 3003281291 ag-struc-frag-safety-base-id: 3003281291 Solvents may damage electrical cables. — Prevent electrical cables from getting in contact with solvents. — Exchange electrical cables after contact with solvents. ag-textnode-version-id: 3602106123 ag-textnode-base-id: 3602106123 Bench Space The module dimensions and weight (see Physical Specifications) allow you to place the module on almost any desk or laboratory bench.
Page 32: Specifications Of The 1260 Infinity Iii Fluorescence Detector (G7121A)
Site Requirements and Specifications Specifications of the 1260 Infinity III Fluorescence Detector (G7121A) ag-textnode-version-id: 9007205351992203 ag-textnode-base-id: 6097251211 Specifications of the 1260 Infinity III Fluorescence Detector (G7121A) Table 2: Physical Specifications of the 1260 Infinity III Fluorescence Detector (G7121A) Type Specification Comments Weight 11.9 kg (26.2 lbs) Dimensions 140 x 396 x 436 mm (height ×...
Page 33 Site Requirements and Specifications Specifications of the 1260 Infinity III Fluorescence Detector (G7121A) Type Specification Comments Single wavelength operation • RAMAN (H O) > 500 (noise reference measured at signal) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell • RAMAN (H O) >...
Page 34 Site Requirements and Specifications Specifications of the 1260 Infinity III Fluorescence Detector (G7121A) Type Specification Comments Safety features and Leak detection, safe leak handling, leak maintenance output signal for shutdown of the pumping system. No hazardous voltages in major maintenance areas. Extensive diagnostics, error detection and display with Agilent InfinityLab Assist and Agilent Lab Advisor software.
Page 35: Specifications Of The 1260 Infinity Iii Fluorescence Detector Spectra (G7121B)
Site Requirements and Specifications Specifications of the 1260 Infinity III Fluorescence Detector Spectra (G7121B) ag-textnode-version-id: 9007205351994763 ag-textnode-base-id: 6097253771 Specifications of the 1260 Infinity III Fluorescence Detector Spectra (G7121B) Table 4: Physical Specifications of the 1260 Infinity III Fluorescence Detector (G7121B) Type Specification Comments Weight 11.9 kg (26.2 lbs) Dimensions 140 x 396 x 436 mm...
Page 36 Site Requirements and Specifications Specifications of the 1260 Infinity III Fluorescence Detector Spectra (G7121B) Type Specification Comments Single wavelength operation • RAMAN (H O) > 500 (noise reference measured at signal) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell • RAMAN (H O) > 3000 ...
Page 37 Site Requirements and Specifications Specifications of the 1260 Infinity III Fluorescence Detector Spectra (G7121B) Type Specification Comments Flow cells Standard: 8 µL volume and 20 bar (2 MPa) pressure maximum, fused silica block Optional: • Bio-inert: 8 µL volume and 20 bar (2 MPa) pressure maximum, (pH 1-12) •...
Page 38: Specification Conditions
Site Requirements and Specifications Specification Conditions ag-textnode-version-id: 3586911755 ag-textnode-base-id: 3586911755 Specification Conditions • Standard flow cell • Standard Photomultiplier • Using Agilent Lab Advisor, see Raman ASTM Signal-to-Noise Test page 139. Fluorescence Detectors User Manual...
Page 39: Installation
ag-textnode-version-id: 9007205352088715 ag-textnode-base-id: 6097347723 Installation The installation of the module will be done by an Agilent service representative. In this chapter, only installation of user-installable options and accessories are described. Installing Capillaries 40 Install Capillaries 40 Handling Leak and Waste 44 Drain Connectors Installation 47 Waste Concept 52 Waste Guidance 52 Leak Sensor 53...
Page 40: Installing Capillaries
Installation Installing Capillaries ag-textnode-version-id: 45036000351652875 ag-textnode-base-id: 4077947915 Installing Capillaries This section provides information on how to install capillaries and fittings. Fluorescence Detectors User Manual...
Page 41: Install Capillaries
Installation Installing Capillaries ag-textnode-version-id: 9007202257747211 ag-textnode-base-id: 3003006219 Install Capillaries Capillaries and connections depend on which system is installed. As you move to smaller-volume, high-efficiency columns, you will want to use NOTE narrow id tubing, as opposed to the wider id tubing used for conventional HPLC instruments.
Page 42 Installation Installing Capillaries From 5500-1264 (Capillary Ti 0.17 mm x 500 mm, SL/SLV) Pump Multisampler G5667-81005 (Capillary PK/ST 0.17 mm x 500 mm, RLO/RLO Multisampler (Bio-inert)) 5067-4741 (ZDV union (Bio-inert)) Capillary Bio-inert Heat Exchanger G7116-60041 (Quick Connect Heat Exchanger Bio-inert) 0890-1763 (Capillary PEEK 0.18 mm x 1.5 m) and Column/MCT Valve Detector 5063-6591 (PEEK Fittings 10/PK) 5062-8535 (Waste accessory kit (Flow Cell to waste)) Waste 5062-2462 (Tube PTFE 0.7 mm x 5 m, 1.6 mm od)
Page 43 Installation Installing Capillaries 3 Carefully slide the ferrule components on after the nut and then finger-tighten the assembly while ensuring that the tubing is completely seated in the bottom of the end fitting. 4 Use a stable port installed to the module to gently tighten the fitting facing to the module.
Page 44: Handling Leak And Waste
36028800545451531 ag-textnode-base-id: 3526487563 Handling Leak and Waste The Agilent InfinityLab LC Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed. The solvent cabinet is designed to store a maximum volume of 8 L solvent. The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 L.
Page 45 Installation Handling Leak and Waste Figure 19: Infinity III Leak Waste Concept (Flex Bench installation) Fluorescence Detectors User Manual...
Page 46 Installation Handling Leak and Waste Figure 20: Infinity III Single Stack Leak Waste Concept (bench installation) Fluorescence Detectors User Manual...
Page 47 Installation Handling Leak and Waste Figure 21: Infinity III Two Stack Leak Waste Concept (bench installation) The waste tube connected to the leak plane outlet on each of the bottom instruments guides the solvent to a suitable waste container. Fluorescence Detectors User Manual...
Page 48: Drain Connectors Installation
Drain Connectors (available only as Drain Connectors Kit 5004-0000) have been developed to improve leak drainage for low flow leaks of high viscosity solvents (for example, isopropanol) in Agilent InfinityLab LC Series Systems. Install these parts to modules where they are missing (usually preinstalled).
Page 49 Installation Handling Leak and Waste Table 8: Compatibility of drain connectors and modules Drain Connector Type Compatible Module Compatible Module Type Double G7116A/B Column Compartment Single G7114A/B Detector G7115A G7117A/B/C G7121A/B G7162A/B G7165A G7129A/B/C Sampler G7167A/B/C G5668A G7137A G7157A G4767A G7122A Degasser G7104A/C Pump...
Page 50 Installation Handling Leak and Waste In case of incorrect installation, drain connectors cannot fully perform the NOTE intended function. It is not required to power off the HPLC stack to install Single and Double Drain NOTE Connectors. The installation of the connectors does not affect the analysis performed during the installation.
Page 51 Installation Handling Leak and Waste 1 Align the ring with the leak drain outlet of the module, press slightly with the fingers, and slide the connector along the leak drain outlet until it is aligned with the front of the leak drain. Make sure that the following requirements are covered: •...
Page 52: Waste Concept
Installation Handling Leak and Waste ag-textnode-version-id: 9007202332686987 ag-textnode-base-id: 3077945995 Waste Concept Agilent recommends using the 5043-1221 (6 L waste can with 1 Stay Safe cap GL45 with 4 ports) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid. ag-textnode-version-id: 3596422027 ag-textnode-base-id: 3596422027 Waste Guidance...
Page 53: Leak Sensor
Installation Handling Leak and Waste The waste drainage must go straight into the waste containers. The waste flow NOTE must not be restricted at bends or joints. ag-textnode-version-id: 3002300811 ag-textnode-base-id: 3002300811 Leak Sensor Solvent incompatibility CAUTION ag-struc-frag-safety-version-id: 3004406283 ag-struc-frag-safety-base-id: 3004406283 The solvent DMF (dimethylformamide) leads to corrosion of the leak sensor.
Page 54: Connecting Modules And Control Software
WARNING ag-struc-frag-safety-version-id: 3822076043-1 ag-struc-frag-safety-base-id: 3822076043 Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury. — Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
Page 55: Using The Module
ag-textnode-version-id: 9007205352449675 ag-textnode-base-id: 6097708683 Using the Module This chapter provides information on how to use the module. General Information 56 Turn On/Off 56 Status Indicators 58 Preparation of the System 60 Prepare a Run 60 Prime and Purge the System 67 Preparing the Module 69 Set Up the Detector with Agilent Open Lab ChemStation 69 The Detector User Interface 70 Detector Control Settings 72 Method Parameter Settings 72...
Page 56: General Information
Using the Module General Information ag-textnode-version-id: 27021601839561739 ag-textnode-base-id: 4075338763 General Information ag-textnode-version-id: 18014401511325195 ag-textnode-base-id: 3001843211 Turn On/Off This procedure exemplarily shows an arbitrary LC stack configuration. Fluorescence Detectors User Manual...
Page 57 Using the Module General Information 2 On/Off switch: On 3 Turn instrument On/Off with the control software. Fluorescence Detectors User Manual...
Page 58: Status Indicators
Using the Module General Information 4 On/Off switch: Off ag-textnode-version-id: 27021600810636555 ag-textnode-base-id: 3046413579 Status Indicators The module status indicator indicates one of six possible module conditions. Fluorescence Detectors User Manual...
Page 59 Using the Module General Information Figure 23: Arbitrary LC stack configuration (example) Idle Run mode Not-ready. Waiting for a specific pre-run condition to be reached or completed. Error mode - interrupts the analysis and requires attention (for example, a leak or defective internal components).
Page 60: Preparation Of The System
Using the Module Preparation of the System ag-textnode-version-id: 54043199514651787 ag-textnode-base-id: 3986205835 Preparation of the System ag-textnode-version-id: 9007203772428555 ag-textnode-base-id: 4517687563 Prepare a Run This procedure exemplarily shows how to prepare a run. Parameters as shown in the screenshots may vary, depending on the system installed. Toxic, flammable and hazardous solvents, samples and reagents WARNING ag-struc-frag-safety-version-id: 3004430475-1...
Page 61 Using the Module Preparation of the System 1 Switch on the detector. 2 Fill the solvent bottles with adequate solvents for your application. 3 Place solvent tubings with bottle head assemblies into the solvent bottles. 4 Place solvent bottles into the solvent cabinet. Fluorescence Detectors User Manual...
Page 62 Using the Module Preparation of the System 5 Solvent bottle filling dialog (in the software). Fluorescence Detectors User Manual...
Page 63 Using the Module Preparation of the System 6 Purge the pump. For details on priming and purging, refer to the technical note Best Practices for NOTE Using an Agilent LC System Technical Note (InfinityLab-BestPractice-en- SD-29000194.pdf, SD-29000194). 7 Change solvent type if necessary. Fluorescence Detectors User Manual...
Page 64 Using the Module Preparation of the System 8 Choose the tray format of the sampler. Fluorescence Detectors User Manual...
Page 65 Using the Module Preparation of the System 9 Add a new column. 10 Enter the column information. Fluorescence Detectors User Manual...
Page 66 Using the Module Preparation of the System 11 Select the column in the Method settings of the column compartment. 12 Set the detector parameters according to the needs of your method. Fluorescence Detectors User Manual...
Page 67 Using the Module Preparation of the System Fluorescence Detectors User Manual...
Page 68: Prime And Purge The System
Using the Module Preparation of the System ag-textnode-version-id: 9007202538343307 ag-textnode-base-id: 3283602315 Prime and Purge the System When the solvents have been exchanged or the pumping system has been turned off for a certain time (for example, overnight) oxygen will re-diffuse into the solvent channel between the solvent reservoir, vacuum degasser (when available in the system) and the pump.
Page 69: Preparing The Module
Using the Module Preparing the Module ag-textnode-version-id: 9007203240570891 ag-textnode-base-id: 3985829899 Preparing the Module ag-textnode-version-id: 3586919947 ag-textnode-base-id: 3586919947 Set Up the Detector with Agilent Open Lab ChemStation The setup of the detector is shown with the Agilent OpenLab ChemStation C.01.07 and Driver A.02.14. This section describes the detector settings only.
Page 70: The Detector User Interface
Using the Module Preparing the Module ag-textnode-version-id: 3586924427 ag-textnode-base-id: 3586924427 The Detector User Interface Within the detector GUI, there are active areas. If you move the mouse cursor across the icons the cursor will change. 1. Lamp: turn on and off of UV-lamp 2.
Page 71 Using the Module Preparing the Module Module Status shows Run / Ready / Error state and “Not Ready text” or “Error text” • Error (red) • Not ready (yellow) • Ready (green) • Pre run, Post run (purple) • Run (blue) •...
Page 72: Detector Control Settings
Using the Module Preparing the Module ag-textnode-version-id: 3586928907 ag-textnode-base-id: 3586928907 Detector Control Settings The figure shows the default settings. • Lamps: can be turned ON/OFF. • Response Multiply Factor: 1 • Analog Output Range: can be set to either 100 mV or 1 Vfull scale, for additional settings see Analog Output (under Method Parameter Settings on page 72).
Page 73 Using the Module Preparing the Module Figure 25: Method parameter settings Fluorescence Detectors User Manual...
Page 74: Advanced Settings
Using the Module Preparing the Module ag-textnode-version-id: 3586937867 ag-textnode-base-id: 3586937867 Advanced Settings Signal A You can define the wavelengths of the excitation and emission and specify signal acquisition. Limits (Ex and Em): 200 to 1200 nm in steps of 1 nm. NOTE: The emission wavelength should be at least 10 nm greater than the excitation wavelength NOTE: Addition signals B, C, D can be added via Mulitiple Wavelength mode (G721B ONLY).
Page 75 Using the Module Preparing the Module Stoptime/Posttime The Stoptime is the time where either the complete system stops (As Pump/Injector) or the module (if different from system stop time). The data collection is stopped at this time. A Posttime period can be used to allow module’s items to equilibrate (e.g.
Page 76: Acquire Spectra (G7121B Only)
Using the Module Preparing the Module ag-textnode-version-id: 3586942347 ag-textnode-base-id: 3586942347 Acquire Spectra (G7121B Only) Fluorescence Detectors User Manual...
Page 77 Using the Module Preparing the Module Acquire Spectra (G7121B ONLY) If you choose multiple excitation or emission wavelengths, you can also choose to acquire spectra. None: No spectra are taken. Apex: A spectrum is acquired at the apex of the peak. All in Peak: All spectra within the peak are acquired.
Page 78: Special Settings
Using the Module Preparing the Module ag-textnode-version-id: 3586946827 ag-textnode-base-id: 3586946827 Special Settings Detection Mode Default: Fluorescence Choose the Fluorescence Mode option to measure luminescence from samples that emit fluorescence. Choose the Phosphorescence Mode option to measure luminescence from samples that emit phosphorescence. When you choose to switch on the Phosphorescence detection mode, the two parameters Delay and Gate are activated.
Page 79 Using the Module Preparing the Module The Lamp section allows you to specify how the lamp is used in an analysis. Select Only On During Run to specify that the lamp is switched on only during the run time, and is switched off when the run finishes. Select Economy Mode to run the lamp in a low frequency, low current mode.
Page 80: Time Table
Using the Module Preparing the Module ag-textnode-version-id: 3586951307 ag-textnode-base-id: 3586951307 Time Table Time Table You may set up time events to change functions with their parameters over the run time. Add lines as required. Time Limits: 0.00 – 99999.00 min in steps of 0.01 min. Via the buttons in the bottom area, time table lines can be added, removed, cut copied, pasted or completely cleared.
Page 81: Optimizing The Performance Of The Module
ag-textnode-version-id: 9007203315049995 ag-textnode-base-id: 4060309003 Optimizing the Performance of the Module This chapter provides information on how to optimize the module. Method Development 82 Step 1: Check the LC System for Impurities 82 Step 2: Optimize Limits of Detection and Selectivity 83 Step 3: Set Up Routine Methods 92 Example: Optimization for Multiple Compounds 96 Setting the Chromatographic Conditions 96 Evaluating the System Background 102...
Page 82: Method Development
Optimizing the Performance of the Module Method Development ag-textnode-version-id: 3585992331 ag-textnode-base-id: 3585992331 Method Development Fluorescence detectors are used in liquid chromatography when superior limits of detection and selectivity are required. Thorough method development, including spectra acquisition, is fundamental to achieve good results. This chapter describes three different steps that can be taken with the Agilent fluorescence detector.
Page 83 Optimizing the Performance of the Module Method Development Figure 26 on page 83 shows a sample of slightly impure water which was planned for use as mobile phase. The area where fluorescence of the contaminated water sample can be seen is between the stray light areas: the first- and second-order Raleigh stray light and Raman stray light.
Page 84 Optimizing the Performance of the Module Method Development The traditional approach is to extract an appropriate excitation wavelength from the UV spectrum that is similar to the fluorescence excitation spectrum (see Figure 27 on page 84) and to record the emission spectrum. Then with an optimum emission wavelength determined, the excitation spectrum is acquired.
Page 85 Optimizing the Performance of the Module Method Development Procedure III - Use an Agilent 1200 Infinty Series FLD/DAD combination and acquire UV/Visible spectra (equivalent to excitation spectra) with the DAD and emission spectra with the FLD-both in a single run. Procedure I - Take a fluorescence scan Because fluorescence spectra traditionally have not been easily available with previous LC fluorescence detectors, standard fluorescence spectrophotometers have been used in the past to acquire spectral information for unknown...
Page 86 Optimizing the Performance of the Module Method Development straylight 1. order 350 nm Ex 315 nm Ex 250 nm Ex Ex axis Em axis Figure 28: Characterization of a pure compound from a fluorescence scan Fluorescence Detectors User Manual...
Page 87 Optimizing the Performance of the Module Method Development Procedure II - Take two LC runs with the FLD The conditions for the separation of organic compounds such as polyaromatic nuclear hydrocarbons (PNAs) are well described in various standard methods, including commonly used EPA and DIN methods. Achieving the best detection levels requires checking for the optimum excitation and emission wavelengths for all compounds.
Page 88 Optimizing the Performance of the Module Method Development Flow rate 0.4 ml/min Column temperature 18 °C Injection volume 5 µl FLD settings PMT 12, response time 4 s, step size 5 nm 1 Naphthalene 8 Benz(a)anthracene This shows the 2 Acenaphthene 9 Chrysene isofluorescence plot 3 Fluorene 10 Benzo(b)fluoranthene of emission spectra...
Page 89 Optimizing the Performance of the Module Method Development 1 Naphthalene 8 Benz(a)anthracene 2 Acenaphthene 9 Chrysene 3 Fluorene 10 Benzo(b)fluoranthene 4 Phenanthrene 11 Benzo(k)fluoranthene 5 Anthracene 12 Benz(a)pyrene 6 Fluoranthene 13 Dibenzo(ah)anthracene 7 Pyrene 14 Benzo(g,h,i)perylene 15 Indeno(1,2,3-cd)pyrene Time [min] Exitation spectra Emission...
Page 90 Optimizing the Performance of the Module Method Development Procedure III - Make a single run with a DAD/FLD combination For most organic compounds, UV-spectra from diode array detectors are nearly identical to fluorescence excitation spectra. Spectral differences are caused by specific detector characteristics such as spectral resolution or light sources.
Page 91 Optimizing the Performance of the Module Method Development The two upper 2-amino-3-OH-phenazine traces are obtained Unknown 2,3-diaminophenazine using two different excitation wavelengths. The lower trace is a pure standard of the known impurities. Standard Time [min] Figure 32: Qualitive analysis of MBC (2-benzimidazole carbamic acid methylester) and impurities Table 14: Conditions for analysis of DAP and MBC according to figures above Column Zorbax SB, 2 x 50 mm, PNA, 5 µm...
Page 92: Step 3: Set Up Routine Methods
Optimizing the Performance of the Module Method Development ag-textnode-version-id: 3586001291 ag-textnode-base-id: 3586001291 Step 3: Set Up Routine Methods In routine analysis, sample matrices can have a significant influence on retention times. For reliable results, sample preparation must be thorough to avoid interferences or LC methods must be rugged enough.
Page 93 Optimizing the Performance of the Module Method Development Flow rate 0.4 mL/min Column temperature 22 °C Injection volume 2 µL FLD settings PMT 12 , response time 4 s The upper trace was 1 excitation WL at 260 nm 1 Naphthalene 8 Benz(a)anthracene received with 4 emission WL at 350, 420, 2 Acenaphthene 9 Chrysene...
Page 94 Optimizing the Performance of the Module Method Development compared automatically with library data. Table 16 on page 94 illustrates this principle using a PNA analysis. The match factor given in the report for each peak indicates the degree of similarity between the reference spectrum and the spectra from a peak.
Page 95 Optimizing the Performance of the Module Method Development Meas. Library CalTbl Signal Amount Purity Match Libary Name RetTime [min] [min] [min] [ng] Factor 19.200 19.100 20.329 6.03334e-1 Benzo(g,h,i)perylene@em 20.106 20.000 21.291 9.13648e-2 Indeno(1,2,3-c,d)pyrene@em Fluorescence Detectors User Manual...
Page 96: Example: Optimization For Multiple Compounds
Optimizing the Performance of the Module Example: Optimization for Multiple Compounds ag-textnode-version-id: 3585976203 ag-textnode-base-id: 3585976203 Example: Optimization for Multiple Compounds Using PNAs as a sample, this example uses the described scanning functions. Fluorescence Detectors User Manual...
Page 97: Setting The Chromatographic Conditions
Optimizing the Performance of the Module Example: Optimization for Multiple Compounds ag-textnode-version-id: 3585981195 ag-textnode-base-id: 3585981195 Setting the Chromatographic Conditions This example uses the following chromatographic conditions (the detector settings are shown in Detector settings for emission scan). Table 17: Chromatographic Conditions Mobile phases A = water = 50 % B = Acetonitrile = 50 %...
Page 98 Optimizing the Performance of the Module Example: Optimization for Multiple Compounds Select a Excitation wavelength in the low UV (230...260 nm). This will cover nearly all fluorescence in your sample. DO NOT select additional emission wavelengths (B, C, D). Doing so will increase the scan time and will lower the performance.
Page 99 Optimizing the Performance of the Module Example: Optimization for Multiple Compounds 2 Load the signal. (In this example just the time range of 13 min is displayed). Figure 35: Chromatogram from Emissions Scan 3 Use the isoabsorbance plot and evaluate the optimal emission wavelengths, shown in the table below.
Page 100 Optimizing the Performance of the Module Example: Optimization for Multiple Compounds Peak # Time Emission Wavelength 10.6 min 445 nm 11.23 min 385 nm 4 Using the settings and the timetable (from previous page), do a second run for the evaluation of the optimal excitation wavelength. DO NOT select additional emission wavelengths (B, C, D).
Page 101 Optimizing the Performance of the Module Example: Optimization for Multiple Compounds 6 Load the signal. Figure 38: Chromatogram - Excitation Scan at Reference Wavelength 260/330 nm 7 Use the isoabsorbance plot and evaluate the optimal excitation wavelengths (in this example just in the time range of 13 minutes). Figure 39: Isoabsorbance Plot - Excitation The table below shows the complete information about emission (from Setting the Chromatographic Conditions, step 3...
Page 102 Optimizing the Performance of the Module Example: Optimization for Multiple Compounds Peak # Time Emission Wavelength Excitation Wavelength 8.5 min 360 nm 245 nm 10.7 min 445 nm 280 nm 11.3 min 385 nm 270 / 330 nm Fluorescence Detectors User Manual...
Page 103: Evaluating The System Background
Optimizing the Performance of the Module Example: Optimization for Multiple Compounds ag-textnode-version-id: 3585984907 ag-textnode-base-id: 3585984907 Evaluating the System Background The example below uses water. 1 Pump solvent through your system. 2 Set the fluorescence scan range under FLD special setpoints according to your needs.
Page 104 Optimizing the Performance of the Module Example: Optimization for Multiple Compounds 3 Set PMT gain to 16. The wavelength range and step number defines the duration. Using the maximum range, the scan would take approximately 10 minutes. Figure 40: FLD special settings 4 Define a data file name and take a fluorescence scan.
Page 105: Optimization Overview
Optimizing the Performance of the Module Optimization Overview ag-textnode-version-id: 3585962763 ag-textnode-base-id: 3585962763 Optimization Overview Some features (e.g. spectrum acquisition, multi-wavelength detection) described NOTE in this chapter are not available on the 1260 Infinity III Fluorescence Detector (G7121A). PMT Gain Test NOTE The PMT Gain test is not available in the Agilent CDS (OpenLAB CDS, OpenLAB CDS Chemstation Edition, OpenLAB EZChrom Edition) and G4208A Instant Pilot.
Page 106 Optimizing the Performance of the Module Optimization Overview Most fluorescent active molecules absorb at 230 nm (see Finding the Best Wavelengths on page 111). Set the excitation wavelength to 230 nm and on- line scan the emission spectra (multi-emission mode). Then set the determined emission wavelength and perform a multi-excitation scan (multi- excitation mode) to find the best excitation wavelength.
Page 107: How To Collect Spectra With Modes Spectra All In Peak And Apex Spectra Only
Optimizing the Performance of the Module How to Collect Spectra with Modes SPECTRA ALL IN PEAK and APEX SPECTRA ONLY ag-textnode-version-id: 3585988619 ag-textnode-base-id: 3585988619 How to Collect Spectra with Modes SPECTRA ALL IN PEAK and APEX SPECTRA ONLY This section describes how to overcome a malfunction in the current implementation of the Agilent OpenLAB CDS with the Fluorescence Detector (G7121B).
Page 108 Optimizing the Performance of the Module How to Collect Spectra with Modes SPECTRA ALL IN PEAK and APEX SPECTRA ONLY 1 Set the parameters THRS, PDPW and PKWD accordingly to the current chromatogram. Best results for collecting peak triggered spectra are gathered when PDPW is 2 steps lower than PKWD, see Table 19 on page 122.
Page 109 Optimizing the Performance of the Module How to Collect Spectra with Modes SPECTRA ALL IN PEAK and APEX SPECTRA ONLY • The peak-detector works online on the current chromatogram. This means that begin/apex/end of a peak is recognized with delay. Additionally the points of spectra are sequentially acquired.
Page 110: Design Features Help Optimization
Check Performance Before You Start Before you start you should check that your detector is performing according to the specifications published by Agilent Technologies. Your normal LC grade solvents may give good results most of the time but our experience shows that baseline noise can be higher with LC grade solvents than with fluorescence grade solvents.
Page 111: Finding The Best Wavelengths
Optimizing the Performance of the Module Finding the Best Wavelengths ag-textnode-version-id: 3585922187 ag-textnode-base-id: 3585922187 Finding the Best Wavelengths The most important parameters to be optimized in fluorescence detection are the excitation and emission wavelengths. Generally, it is assumed that the best excitation wavelength can be taken from the excitation spectrum acquired on a spectrofluorimeter.
Page 112 Optimizing the Performance of the Module Finding the Best Wavelengths Figure 42: Scan Orthophthalaldehyde Derivative of Alanine When you are looking for the wavelength by scanning, scan over the whole range. As this example shows a maximum may be found in a completely different wavelength range.
Page 113: Finding The Best Signal Amplification
Figure 43 on page 113 the PMTGAIN was gradually raised from 4 up to 11 (the peak is from the Agilent Technologies isocratic sample which was diluted 1000 times). With increasing PMTGAIN there was an improvement in signal-to- noise up to 10 . Above 10 the noise increased proportionately to the signal with no improvement in signal-to-noise.
Page 114: Fld Scaling Range And Operating Conditions
Optimizing the Performance of the Module Finding the Best Signal Amplification ag-textnode-version-id: 3585953803 ag-textnode-base-id: 3585953803 FLD Scaling Range and Operating Conditions When using different FLD • The signal height of individual G7121 FLD modules may exceed the recommended signal range 0 – 100 LU. Under certain circumstances this could lead to clipped peaks.
Page 115: Optimize The Pmt-Gain-Level
Optimizing the Performance of the Module Finding the Best Signal Amplification ag-textnode-version-id: 3585950091 ag-textnode-base-id: 3585950091 Optimize the PMT-Gain-Level Start the PMT-Gain-Test with your operating conditions (used method parameter, EX-/EM-wavelength, solvent, flow rate, …). The resulting PMT value will give you the best signal to noise performance with the maximum usable signal range for this method and this specific instrument.
Page 116 Optimizing the Performance of the Module Finding the Best Signal Amplification 1 Set the PMT-Gain Level Now check with your highest concentration amount, that your highest peak does not clip or overflow. • If this check is ok, you finished the PMT-Gain-Level optimization. Continue with "Set your Luminescence Units in LU".
Page 117: Visualization Of Adc Limits
Optimizing the Performance of the Module Finding the Best Signal Amplification The level of LU is no measure of instrument sensitivity! At the lowest concentration limit (limit of detection), the signal to noise (for example by Raman S/N Test) is the only measure that can accurately be used to compare chromatograms and results and to confirm the performance of the instrument.
Page 118 Optimizing the Performance of the Module Finding the Best Signal Amplification Raw ADC counts The measured light intensity is limited by the max range of the ADC- converter. A filter smoothes the peak making it not clearly visible that the max intensity is reached.
Page 119: Changing The Xenon Flash Lamp Frequency
Optimizing the Performance of the Module Changing the Xenon Flash Lamp Frequency ag-textnode-version-id: 3585931659 ag-textnode-base-id: 3585931659 Changing the Xenon Flash Lamp Frequency Modes The lamp flash frequency can be changed into the following modes: Table 18: Flash Lamp Modes Positioning 296 Hz (Standard), 560 V 63 mJ (18.8 W) 74 Hz (Economy), 560 V 63 mJ (4.7 W)
Page 120 Optimizing the Performance of the Module Changing the Xenon Flash Lamp Frequency • switch to “economy” mode with a certain loss of sensitivity. • a combination of the above. Fluorescence Detectors User Manual...
Page 121: Selecting The Best Response Time
Optimizing the Performance of the Module Selecting the Best Response Time ag-textnode-version-id: 3585967243 ag-textnode-base-id: 3585967243 Selecting the Best Response Time Data reduction using the RESPONSETIME function will increase your signal-to- noise ratio. For example, see Figure 46 on page 121. 8 sec 4 sec 1 sec Figure 46: Finding Best Response Time...
Page 122: Peakwidth Settings
Optimizing the Performance of the Module Selecting the Best Response Time ag-textnode-version-id: 3585971723 ag-textnode-base-id: 3585971723 Peakwidth Settings Do not use peak width shorter than necessary. NOTE Peakwidth enables you to select the peak width (response time) for your analysis. The peak width is defined as the width of a peak, in minutes, at half the peak height.
Page 123: Reducing Stray Light
Optimizing the Performance of the Module Reducing Stray Light ag-textnode-version-id: 3585941131 ag-textnode-base-id: 3585941131 Reducing Stray Light Cut-off filters are used to remove stray light and 2 order or higher stray light by allowing complete transmission above the cut-off and little or no transmission below the cut-off point.
Page 124 Optimizing the Performance of the Module Reducing Stray Light Exitation 300nm Second order light 600nm Stray light no filter filter 280 nm Wavelength [nm] Figure 48: Reducing Stray Light Fluorescence Detectors User Manual...
Page 125: Diagnostics And Troubleshooting
ag-textnode-version-id: 9007205352564235 ag-textnode-base-id: 6097823243 Diagnostics and Troubleshooting This chapter gives an overview of the maintenance, troubleshooting, and diagnostic features available. Diagnostic Features 127 User Interfaces 127 Troubleshooting With HPLC Advisor 127 Overview of Diagnostic Signals 128 Baseline Problems Deriving from Xenon Flash Lamp/Trigger Pack Assembly 128 Wander/Drift Problems Due to Temperature Changes 129 Monitoring of Additional Signals 130...
Page 126 Diagnostics and Troubleshooting Instrument Control 165 EMF - Early Maintenance Feature 166 Spectral Scan (G7121B only) 166 Fluorescence Detectors User Manual...
Page 127: Diagnostic Features
Diagnostics and Troubleshooting Diagnostic Features ag-textnode-version-id: 27021601745768715 ag-textnode-base-id: 3981545739 Diagnostic Features This section gives an overview of the diagnostic features available. ag-textnode-version-id: 18014402312290571 ag-textnode-base-id: 3802808587 User Interfaces InfinityLab Assist InfinityLab Assist provides you with assisted troubleshooting and maintenance at your instrument. If the system in use supports the InfinityLab Assist, follow the instructions provided.
Page 128: Overview Of Diagnostic Signals
Diagnostics and Troubleshooting Diagnostic Features ag-textnode-version-id: 3585826315 ag-textnode-base-id: 3585826315 Overview of Diagnostic Signals The detector has several signals (internal temperatures, signal, reference signal) that can be used to diagnose problems. These can be • baseline problems deriving from the Xenon flash lamp / trigger pack assembly •...
Page 129: Wander/Drift Problems Due To Temperature Changes
Diagnostics and Troubleshooting Diagnostic Features ag-textnode-version-id: 3585836299 ag-textnode-base-id: 3585836299 Problem Verification Perform the following steps to diagnose whether the lamp is the cause of the baseline instability: 1 Ensure that the detector has been properly warmed up, see Overview of Diagnostic Signals on page 128.
Page 130: Monitoring Of Additional Signals
Diagnostics and Troubleshooting Diagnostic Features ag-textnode-version-id: 3585812363 ag-textnode-base-id: 3585812363 Monitoring of Additional Signals The detector has several signals (internal temperatures, Reference only) that can be used for diagnosing problems. These can be • baseline problems deriving from lamp, • wander / drift problems due to temperature changes. For intermittent baseline problems the board temperature should be monitored to see the impact of it to the signal.
Page 131 Diagnostics and Troubleshooting Diagnostic Features Figure 49: Signal plus board temperature (Agilent Lab Advisor) The board temperature (short-/long term) is also stored in the module’s memory and can be retrieved via the Module Info - Signals. Figure 50: Internal board temperature (Agilent Lab Advisor - Module Info - Signals) Fluorescence Detectors User Manual...
Page 132: Overview Of Available Tests And Tools
Diagnostics and Troubleshooting Overview of Available Tests and Tools ag-textnode-version-id: 4060343563 ag-textnode-base-id: 4060343563 Overview of Available Tests and Tools ag-textnode-version-id: 3002950539 ag-textnode-base-id: 3002950539 Tests and Calibrations in Agilent Lab Advisor Use the tests and diagnostic features provided in the Agilent Lab Advisor software to check if your module is working correctly.
Page 133: Maintenance And Troubleshooting Tools Of The Module
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 9007203316498315 ag-textnode-base-id: 4061757323 Maintenance and Troubleshooting Tools of the Module This chapter describes the tests for the module. ag-textnode-version-id: 3586744843 ag-textnode-base-id: 3586744843 Introduction All tests are described based on the Agilent Lab Advisor Software B.02.08. Other user interfaces may not provide any test or just a few.
Page 134 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Product Level - Dark Current Test Basic Advanced - Intensity Test Basic Advanced - Raman ASTM Signal/Noise Test Basic Advanced - Wavelength Accuracy Test Basic Advanced Calibrations - Wavelength Calibration Basic Advanced Tools...
Page 135 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Product Level - Special Commands - Detector Reset Basic Advanced - Clear Error Basic Advanced Statemachines - UV Lamp Advanced Signals - Signal A [LU] (G7121A allows just Signal A) Advanced - Signal B [LU] Advanced...
Page 136: Diagram Of Light Path
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Figure 51: The Lab Advisor shows the available tests ag-textnode-version-id: 3586705547 ag-textnode-base-id: 3586705547 Diagram of Light Path The light path is shown in Figure 52 on page 137. Fluorescence Detectors User Manual...
Page 137: Lamp Intensity Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module EX slit Mirror Emission grating EM slit PMT slit Xenon flash Source lamp lens Photo Excitation Emission lens multiplier grating Flow cell Reference diode Diffuser Detector (FLD) Safety cap Figure 52: Schematic of the light path ag-textnode-version-id: 3586710027 ag-textnode-base-id: 3586710027 Lamp Intensity Test...
Page 138: Lamp Intensity History
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Figure 53: Lamp Intensity Test (Agilent Lab Advisor) The profile can vary from instrument to instrument. It is dependig on the age of NOTE the lamp and the content of the flow cell (use fresh water). UV degradation, especially below 250 nm is significantly higher compared to visible wavelength range.
Page 139: Raman Astm Signal-To-Noise Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Figure 54: Lamp Intensity History (Agilent Lab Advisor under Module Info) ag-textnode-version-id: 3586753803 ag-textnode-base-id: 3586753803 Raman ASTM Signal-to-Noise Test This test verifies the Raman ASTM signal-to-noise for the FLD detectors. Figure 55: Raman ASTM Signal-to-Noise Test (Lab Advisor) Depending on the version of the detector the specification has changed.
Page 140 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module The Dark and Dual WL values are just additional specifications. Only the Raman NOTE value is used for the standard instrument checkout. The specification single wavelength at signal can be measured with the Agilent NOTE Lab Advisor.
Page 141 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Table 26: Settings for Dual Wavelength Specifications (Multi-EM Scan) Time EM_A EM_B Spectra From Step Baseline Spectra 00.00 None Free 20.30 None Free Formulas for the Raman ASTM S/N value (see Figure 56 on page 141 for details): mean_raman...
Page 142 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3586763275 ag-textnode-base-id: 3586763275 Using the Agilent Lab Advisor 1 Set up the HPLC system and the Lab Advisor. 2 Flush the flow cell with clean bi-distilled water. 3 Start the test in the Lab Advisor. Figure 57: Raman ASTM Signal-to-Noise Test (Agilent Lab Advisor) In case of failing this test (as shown above) see Interpretation of the Results...
Page 143: Wavelength Accuracy Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3586758283 ag-textnode-base-id: 3586758283 Interpretation of the Results If the test shows low Raman values, check for: • correctly positioned flow cell, • clean flow cell (flush with clean bi-distilled water), •...
Page 144 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module As result, the maxima should be found at 397 nm ± 3 nm, see following figure. EM = 397 nm EX = 350 nm fixed fixed EX = 350 nm EM = 397 nm +/- 3 nm +/- 3 nm Figure 58: Excitation and Emission Spectrum (expected results)
Page 145 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Figure 60: Example of good EX/EM maxima If the plots do not have a maximum around EX=397 nm and EX=350 nm (±3 nm) the test fails, see figure below. Refer to Interpretation of the Results on page 146.
Page 146 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Figure 61: Example of bad EX/EM maxima (no maximum found) ag-textnode-version-id: 3586771467 ag-textnode-base-id: 3586771467 Interpretation of the Results If the test fails, check for: • correctly positioned flow cell, • clean flow cell (flush with isopropanol and clean bi-distilled water), •...
Page 147: Wavelength Verification And Calibration
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 9007202841507979 ag-textnode-base-id: 3586766987 Wavelength Verification and Calibration New instruments are shipped with a flow cell installed and have a factory NOTE wavelength calibration. If a flow cell is used other than the installed/shipped flow cell: verify that the installed flow cell is fixed very tight, perform a wavelength calibration using Glycogen (part of FLD Calibration Kit (G7121-68001)).
Page 148: Wavelength Calibration Process
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3586830347 ag-textnode-base-id: 3586830347 Wavelength Calibration Process Prior to a wavelength calibration, a wavelength accuracy verification should be NOTE performed, see Wavelength Accuracy Test. If the deviation is more than ±3 nm, the wavelength calibration should be done as described in Wavelength Calibration Procedure...
Page 149 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Table 28: Wavelength Calibration Steps Step Description Duration Preparation max 30 s Excitation rotation scan, full circle 60 s Excitation rotation scan, high resolution 44 s Excitation position scan, low resolution 55 s variable Excitation position scan, high resolution 260 s variable Emission rotation scan, full circle (# of scans depends on the required...
Page 150: Wavelength Calibration Procedure
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 9007202841520651 ag-textnode-base-id: 3586779659 Wavelength Calibration Procedure When • If application requires, or see Table 28 on page 149. Tools required Qty. Description Laboratory balance Parts required Qty. Description G7121-68001 FLD Calibration Kit contains parts below 5063-6597 Calibration Sample, Glycogen...
Page 151 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 2 Preparation of the Flow Cell. a Flush the flow cell with water. b Remove the inlet capillary from the flow cell. c Remove the outlet tubing from the flow cell and install a short (less than 5 cm) waste tubing instead.
Page 152 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Remove the filter. Take the syringe and fix the needle to the syringe. k Suck about 4 mL of the calibration sample into the syringe. Keep the syringe in a horizontal position. m Remove the needle.
Page 153 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 3 Wavelength Calibration. a From the user interface start the FLD wavelength calibration. • Agilent Lab Advisor (preferred option): Under Instrument Control > Special Commands press the Reset grating calibration button, then go for Service &...
Page 154 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module b If a deviation is displayed, press Yes to adjust to new values. The history table will be updated. Figure 64: Wavelength Calibration (Agilent Lab Advisor) Figure 65: Calibration History (Agilent Lab Advisor, under Module Info) Fluorescence Detectors User Manual...
Page 155: Excitation And Emission Grating Resistance History
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Restore flow connections and flush with fresh water at 1.5 mL/min - 2 mL/min NOTE for at least 15-20 min. Prolonged flushing (e.g. overnight flushing) at low flow rates will not be sufficient. 4 Verification using the "Wavelength Accuracy Test".
Page 156 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Figure 66: Resistance History (Agilent Lab Advisor under Module Info) Fluorescence Detectors User Manual...
Page 157: D/A Converter (Dac) Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3586783371 ag-textnode-base-id: 3586783371 D/A Converter (DAC) Test The detector provides analog output of chromatographic signals for use with integrators, chart recorders or data systems. The analog signal is converted from the digital format by the digital-analog-converter (DAC).
Page 158 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 1 Run the D/A Converter (DAC) Test (for further information see Online-Help of user interface). Figure 67: D/A Converter (DAC) Test Figure 68: D/A Converter (DAC) Test – Example of Integrator Plot Running the Test with Instant Pilot The test can be started via the command line.
Page 159: Dark-Current Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3284410763 ag-textnode-base-id: 3284410763 D/A Converter Test failed D/A Converter Test evaluation The noise on the step should be less than 3 µV. Probable cause Suggested actions Bad cable or grounding •...
Page 160 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Figure 70: Dark Current History (Agilent Lab Advisor) ag-textnode-version-id: 3586723467 ag-textnode-base-id: 3586723467 Dark-Current Test Failed Probable cause Suggested actions Defective PMT. • Exchange the PMT. Defective reference diode or • Exchange the FLF board. A/D converter.
Page 161: Using The Built-In Test Chromatogram
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 18014401794165771 ag-textnode-base-id: 3284683787 Using the Built-In Test Chromatogram This function is available from the Agilent ChemStation, Lab Advisor and Instant Pilot. The built-in Test Chromatogram can be used to check the signal path from the detector to the data system and the data analysis or via the analog output to the integrator or data system.
Page 162 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 6 To start a test chromatogram enter in the command line: STRT Figure 71: Test Chromatogram with Agilent Lab Advisor 7 To stop the test chromatogram enter in the command line: STOP The test chromatogram is switched off automatically at the end of a run.
Page 163: Available Tests Vs User Interfaces
Diagnostics and Troubleshooting Available Tests vs User Interfaces ag-textnode-version-id: 3585821835 ag-textnode-base-id: 3585821835 Available Tests vs User Interfaces Depending on the used interface, the available tests and the screens/reports may NOTE vary. Preferred tool should be the Agilent Lab Advisor, see Agilent Lab Advisor Software on page 164.
Page 164: Agilent Lab Advisor Software
Lab Advisor Advanced Lab Advisor Basic is included with every Agilent 1200 Infinity Series and Agilent InfinityLab LC Series instrument. The Lab Advisor Advanced features can be unlocked by purchasing a license key, and include real-time monitoring of instrument actuals, all various instrument signals, and state machines.
Page 165: Other Lab Advisor Functions
Diagnostics and Troubleshooting Other Lab Advisor Functions ag-textnode-version-id: 9007203316275083 ag-textnode-base-id: 4061534091 Other Lab Advisor Functions ag-textnode-version-id: 3586821387 ag-textnode-base-id: 3586821387 Instrument Control The Instrument Control screen provides controls for each of the modules in the selected system. Module information and status are displayed; click to display the module's controls or to hide them.
Page 166 Diagnostics and Troubleshooting Other Lab Advisor Functions ag-textnode-version-id: 3586825867 ag-textnode-base-id: 3586825867 EMF - Early Maintenance Feature The EMFs screen allows you to view and manage the EMF counters for all modules in all systems. ag-textnode-version-id: 3586807947 ag-textnode-base-id: 3586807947 Spectral Scan (G7121B only) The Spectral Scan tool for the fluorescence detector (FLD) allows you to scan a spectrum over a specified wavelength range in a specified mode, and export the data to a csv (comma-separated values) file that can be used in other...
Page 167 Diagnostics and Troubleshooting Other Lab Advisor Functions Figure 74: Spectral Scan FLD Mode • No multi WL: You specify an Excitation wavelength (Ex) and an Emission wavelength (Em). If you select No multi WL, you cannot set up a scan. • Excitation Scan: You specify an Emission wavelength (Em) and the excitation scan range.
Page 168: Error Information
ag-textnode-version-id: 9007205352642059 ag-textnode-base-id: 6097901067 Error Information This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. What Are Error Messages 169 General Error Messages 170 Timeout 170 Shutdown 170 Remote Timeout 171 Lost CAN Partner 172 Leak 173 Leak Sensor Open 174...
Page 169: What Are Error Messages
Error Information What Are Error Messages ag-textnode-version-id: 18014401511832075 ag-textnode-base-id: 3002350091 What Are Error Messages Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs that requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary).
Page 170: General Error Messages
Error Information General Error Messages ag-textnode-version-id: 63050398309715339 ag-textnode-base-id: 3526528395 General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well. ag-textnode-version-id: 9007202257100299 ag-textnode-base-id: 3002359307 Timeout Error ID: 62 The timeout threshold was exceeded.
Page 171: Shutdown
Error Information General Error Messages ag-textnode-version-id: 27021600766713739 ag-textnode-base-id: 3002490763 Shutdown Error ID: 63 An external instrument has generated a shutdown signal on the remote line. The module continually monitors the remote input connectors for status signals. A LOW signal input on pin 4 of the remote connector generates the error message.
Page 172: Remote Timeout
Error Information General Error Messages ag-textnode-version-id: 9007202257270027 ag-textnode-base-id: 3002529035 Remote Timeout Error ID: 70 A not-ready condition is still present on the remote input. When an analysis is started, the system expects all not-ready conditions (for example, a not-ready condition during detector balance) to switch to run conditions within one minute of starting the analysis.
Page 173: Lost Can Partner
Error Information General Error Messages ag-textnode-version-id: 9007202257121675 ag-textnode-base-id: 3002380683 Lost CAN Partner Error ID: 71 During an analysis, the internal synchronization or communication between one or more of the modules in the system has failed. The system processors continually monitor the system configuration. If one or more of the modules is no longer recognized as being connected to the system, the error message is generated.
Page 174: Leak
Error Information General Error Messages ag-textnode-version-id: 27021600766637707 ag-textnode-base-id: 3002414731 Leak Error ID: 64 A leak was detected in the module. The signals from the two temperature sensors (leak sensor and board-mounted temperature-compensation sensor) are used by the leak algorithm to determine whether a leak is present.
Page 175: Leak Sensor Open
Error Information General Error Messages ag-textnode-version-id: 27021600766781835 ag-textnode-base-id: 3002558859 Leak Sensor Open Error ID: 83 The leak sensor in the module has failed (open circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak sensor current to change within defined limits.
Page 176: Leak Sensor Short
Error Information General Error Messages ag-textnode-version-id: 27021600766675979 ag-textnode-base-id: 3002453003 Leak Sensor Short Error ID: 82 The leak sensor in the module has failed (short circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak sensor current to change within defined limits.
Page 177: Compensation Sensor Open
Error Information General Error Messages ag-textnode-version-id: 45035999276327435 ag-textnode-base-id: 3002622475 Compensation Sensor Open Error ID: 81 The ambient-compensation sensor (NTC) on the power switch board in the module has failed (open circuit). The resistance across the temperature compensation sensor (NTC) on the power switch board is dependent on ambient temperature.
Page 178: Compensation Sensor Short
Error Information General Error Messages ag-textnode-version-id: 45035999276369931 ag-textnode-base-id: 3002664971 Compensation Sensor Short Error ID: 80 The ambient-compensation sensor (NTC) on the power switch board in the module has failed (open circuit). The resistance across the temperature compensation sensor (NTC) on the power switch board is dependent on ambient temperature.
Page 179: Fan Failed
Error Information General Error Messages ag-textnode-version-id: 27021601047950859 ag-textnode-base-id: 3283727883 Fan Failed Error ID: 68 Depending on the module, assemblies (e.g. the lamp in the detector) are turned off to assure that the module does not overheat inside. Probable cause Suggested actions Fan cable disconnected.
Page 180: Open Cover
Error Information General Error Messages ag-textnode-version-id: 9007202538630155 ag-textnode-base-id: 3283889163 Open Cover Error ID: 205 The top foam has been removed. The sensor on the main board detects when the top foam is in place. If the foam is removed, the fan is switched off, and the error message is generated. Probable cause Suggested actions The top foam was removed...
Page 181: Cover Violation
Error Information General Error Messages ag-textnode-version-id: 18014401793749899 ag-textnode-base-id: 3284267915 Cover Violation Error ID: 7461 The top foam has been removed. The sensor on the main board detects when the top foam is in place. If the foam is removed while the lamps are on (or if an attempt is made to switch on for example the lamps with the foam removed), the lamps are switched off, and the error message is generated.
Page 182: Eri Messages
Error Information General Error Messages ag-textnode-version-id: 9007202538689547 ag-textnode-base-id: 3283948555 ERI Messages Error ID: 11120, 11121 The ERI (Enhanced Remote Interface) provides two error events related to over current situations on the +5 V and +24 V lines. Probable cause Suggested actions The load on the ERI is too •...
Page 183: Fluorescence Detector Error Messages
Error Information Fluorescence Detector Error Messages ag-textnode-version-id: 9007205354178699 ag-textnode-base-id: 6099437707 Fluorescence Detector Error Messages ag-textnode-version-id: 3586318347 ag-textnode-base-id: 3586318347 Lamp Cover Open Error ID: 6622, 6731 The lamp cover in the optical compartment has been removed. The lamp cannot be turned on while this message is on. Probable cause Suggested actions Lamp cover removed.
Page 184: Flf Board Not Found
Error Information Fluorescence Detector Error Messages ag-textnode-version-id: 3586335499 ag-textnode-base-id: 3586335499 FLF Board Not Found Error ID: 6620, 6730 The FLF board could not be found by the main board (FLM). This message comes together with some other message generated on the FLF board (e.g. Leak, ...).
Page 185: Adc Not Calibrated
Error Information Fluorescence Detector Error Messages ag-textnode-version-id: 3586377995 ag-textnode-base-id: 3586377995 ADC Not Calibrated Error ID: 6621, 6732 The analog-to-digital converter located on the FLF board cannot calibrate. Probable cause Suggested actions Defective ADC or other FLF • Please contact your Agilent service representative. electronics.
Page 186: A/D Overflow
Error Information Fluorescence Detector Error Messages ag-textnode-version-id: 3603857291 ag-textnode-base-id: 3603857291 A/D Overflow Error ID: 6618, 6619 This message is not implemented in firmware revision A.03.66 and below. It indicates an overload situation of the A/D converter (sample signal). The user- interface will show a not-ready condition for the FLD and an info event is written into the logbook.
Page 187: Flash Lamp Current Overflow
Error Information Fluorescence Detector Error Messages ag-textnode-version-id: 3586630027 ag-textnode-base-id: 3586630027 Flash Lamp Current Overflow Error ID: 6704 The lamp current of the xenon flash lamp is monitored constantly. If the current gets too high, an error is generated and the lamp is turned OFF. Probable cause Suggested actions Short-circuit of trigger pack...
Page 188: No Light At Reference Diode Despite Lamp Is On
Error Information Fluorescence Detector Error Messages ag-textnode-version-id: 3586255243 ag-textnode-base-id: 3586255243 No Light at Reference Diode Despite Lamp Is On Error ID: 6721 • Revision A/B/C Front End Board (FLF): • There is no feedback mechanism that checks whether the lamp is ON! If no peaks are shown in the chromatogram, the user-interface shows the module still in Ready.
Page 189: Flash Trigger Lost
Error Information Fluorescence Detector Error Messages ag-textnode-version-id: 3586395147 ag-textnode-base-id: 3586395147 Flash Trigger Lost Error ID: 6722 This message is displayed when the flash trigger is no longer generated. Probable cause Suggested actions Firmware problem. • Reboot the detector (power cycle). Multi Mode Off •...
Page 190: Wavelength Calibration Failed
Error Information Fluorescence Detector Error Messages ag-textnode-version-id: 3586659851 ag-textnode-base-id: 3586659851 Wavelength Calibration Failed Error ID: 6703 This message may show up during a wavelength calibration. If the expected deviation is larger than the specified wavelength accuracy, the message Wavelength Calibration Failed is displayed and the instrument stays in a Not Ready condition.
Page 191: Wavelength Calibration Lost
Error Information Fluorescence Detector Error Messages ag-textnode-version-id: 9007202840953483 ag-textnode-base-id: 3586212491 Wavelength Calibration Lost Error ID: 6691 After exchanging the monochromator assemblies, the calibration factors should be reset to defaults values (a new FLM board comes with default values). In this case Wavelength Calibration Lost is displayed and the instrument stays in a Not Ready condition.
Page 192: Flow Cell Removed
Error Information Fluorescence Detector Error Messages ag-textnode-version-id: 3603865995 ag-textnode-base-id: 3603865995 Flow Cell Removed Error ID: 6616, 6702, 6760 The detector has an automatic cell recognition system. When the flow cell is removed, the lamp is turned off and a NOT READY condition exists. If the flow cell is removed during an analysis, a SHUT DOWN is generated.
Page 193 Error Information Fluorescence Detector Error Messages ag-textnode-version-id: 3603926667 ag-textnode-base-id: 3603926667 Motor or Encoder Not Found Error ID: 6705, 6706 During initialization of the detector, the excitation and emission monochromator are activated. Probable cause Suggested actions Encoder cables mixed on • Check encoder connections to FLM. FLM board.
Page 194 Error Information Fluorescence Detector Error Messages ag-textnode-version-id: 3604027019 ag-textnode-base-id: 3604027019 Encoder Index Not Found Error ID: 6707, 6708 During initialization of the detector, the excitation- and emission monochromator are activated and the encoder should generate an index. Probable cause Suggested actions Encoder defective.
Page 195 Error Information Fluorescence Detector Error Messages ag-textnode-version-id: 3604037259 ag-textnode-base-id: 3604037259 Motor Friction Too High Error ID: 6709, 6710 During initialization of the detector, the excitation and emission grating resistance test provides the resistance history of the excitation and the emission grating drives.
Page 196 Error Information Fluorescence Detector Error Messages ag-textnode-version-id: 3604046219 ag-textnode-base-id: 3604046219 Motor Position Not Found Error ID: 6711, 6712 When the wavelength is changed the monochromator should move to the new position. The position could not be found. Probable cause Suggested actions Defective monochromator •...
Page 197 Error Information Fluorescence Detector Error Messages ag-textnode-version-id: 3604056587 ag-textnode-base-id: 3604056587 Motor Position Lost Error ID: 6713, 6714 A mechanical shock to the instrument during operation may cause a movement of the monochromator. The position is lost and the lamp will turn off. Probable cause Suggested actions Short mechanical shock.
Page 198 Error Information Fluorescence Detector Error Messages ag-textnode-version-id: 3604092427 ag-textnode-base-id: 3604092427 Motor Speed Too Low Error ID: 6715, 6716 For proper operation the monochromator gratings must run at a certain constant revolution. Probable cause Suggested actions Revolution too low. • Exchange the monochromator assembly. Fluorescence Detectors User Manual...
Page 199 Error Information Fluorescence Detector Error Messages ag-textnode-version-id: 3586582795 ag-textnode-base-id: 3586582795 Motor Speed Unstable Error ID: 6717, 6718 For proper operation the monochromator gratings must run at a certain constant revolution. Probable cause Suggested actions Defective monochromator • Exchange the monochromator assembly. assembly.
Page 200 Error Information Fluorescence Detector Error Messages ag-textnode-version-id: 3604124683 ag-textnode-base-id: 3604124683 Motor Encoder Index Wrong Error ID: 6719, 6720 The actual encoder pattern is checked against a known pattern. Probable cause Suggested actions Encoder was replaced and • Reset pattern via user interface and recalibrate. has a different pattern or no reset of pattern was made.
Page 201: Maintenance
ag-textnode-version-id: 9007203332884747 ag-textnode-base-id: 4078143755 Maintenance This chapter provides general information on maintenance of the module. Introduction to Maintenance 202 Safety Information Related to Maintenance 203 Overview of Maintenance 205 Cleaning the Module 206 Remove and Install Doors 207 Exchange a Flow Cell 210 How to Use the Cuvette 216 Flow Cell Flushing 217 Correcting Leaks 218 Replace Leak Handling System Parts 220...
Page 202: Introduction To Maintenance
Maintenance Introduction to Maintenance ag-textnode-version-id: 3284748811 ag-textnode-base-id: 3284748811 Introduction to Maintenance The module is designed for easy maintenance. Maintenance can be done from the front with module in place in the system. There are no serviceable parts inside. NOTE Do not open the module. ag-struc-frag-safety-version-id: 3369125003 ag-struc-frag-safety-base-id: 3369125003 Fluorescence Detectors User Manual...
Page 203: Safety Information Related To Maintenance
Maintenance Safety Information Related to Maintenance ag-textnode-version-id: 18014402382780683 ag-textnode-base-id: 3873298699 Safety Information Related to Maintenance Eye damage by detector light WARNING ag-struc-frag-safety-version-id: 9007202540437131 ag-struc-frag-safety-base-id: 3285696139 Eye damage may result from directly viewing the UV-light produced by the lamp of the optical system used in this product. —...
Page 204 Maintenance Safety Information Related to Maintenance Toxic, flammable and hazardous solvents, samples and reagents WARNING ag-struc-frag-safety-version-id: 3003809803 ag-struc-frag-safety-base-id: 3003809803 The handling of solvents, samples and reagents can hold health and safety risks. — When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
Page 205: Overview Of Maintenance
Maintenance Overview of Maintenance ag-textnode-version-id: 3586967691 ag-textnode-base-id: 3586967691 Overview of Maintenance The following pages describe maintenance (simple repairs) of the detector that can be carried out without opening the main cover. Table 29: Simple repairs Procedure Typical Frequency Notes Flow cell exchange If application requires a different Complete Assembly flow cell type or if defective.
Page 206: Cleaning The Module
Maintenance Cleaning the Module ag-textnode-version-id: 3002023307 ag-textnode-base-id: 3002023307 Cleaning the Module To keep the module case clean, use a soft cloth slightly dampened with water, or a solution of water and mild detergent. Avoid using organic solvents for cleaning purposes. They can cause damage to plastic parts. Liquid dripping into the electronic compartment of your module can cause WARNING shock hazard and damage the module...
Page 207: Remove And Install Doors
Maintenance Remove and Install Doors ag-textnode-version-id: 9007202970991243 ag-textnode-base-id: 3716250251 Remove and Install Doors When • The instrument doors or the hinges are broken. Tools required Qty. Description 5023-3138 Reversible Screwdriver + Blade 1,0 x 5,5 Parts required Qty. Description (Infinity III) 5004-3140 Door Kit Infinity III 140mm Latched Parts required...
Page 208 Maintenance Remove and Install Doors 1 Press the release buttons and pull the front door out. 2 For the Installation of the front door, insert the hinges into their guides and push the door in until the release buttons click into their final position. Fluorescence Detectors User Manual...
Page 209 Maintenance Remove and Install Doors Fluorescence Detectors User Manual...
Page 210: Exchange A Flow Cell
Maintenance Exchange a Flow Cell ag-textnode-version-id: 9007202841785227 ag-textnode-base-id: 3587044235 Exchange a Flow Cell For bio-inert modules use bio-inert parts only! When • If an application needs a different type of flow cell or the flow cell is defective (leaky). Tools required Qty.
Page 211 Maintenance Exchange a Flow Cell New instruments are shipped with a flow cell installed and have a factory NOTE wavelength calibration. If a flow cell is used other than the installed/shipped flow cell: — verify that the installed flow cell is fixed very tight, —...
Page 212 Maintenance Exchange a Flow Cell 3 Unscrew the thumb screws of the flow cell. 4 Remove the flow cell from the compartment. There are no parts that can be replaced on the flow cell. If defective (leaky) the NOTE flow cell has to be replaced completely. Fluorescence Detectors User Manual...
Page 213 Maintenance Exchange a Flow Cell 5 Insert the flow cell and tighten the thumb screws strongly. If the cell is not completely in its end position and tightened strongly this could NOTE result into a wavelength shift. The label attached to the flow cell provides information on part number, cell NOTE volume and maximum pressure.
Page 214 Maintenance Exchange a Flow Cell 7 Reconnect the capillaries to the flow cell. DO NOT install the inlet capillary to the outlet connection of the flow cell. This will NOTE result in poor performance or damage. If an additional detector is added to the system, the fluorescence detector should NOTE be the last detector in the flow path except for evaporative detectors, like LC- MSD.
Page 215 Maintenance Exchange a Flow Cell 9 Close the doors of the module. 10 Perform a Wavelength Calibration. Fluorescence Detectors User Manual...
Page 216: How To Use The Cuvette
Maintenance How to Use the Cuvette ag-textnode-version-id: 3586960267 ag-textnode-base-id: 3586960267 How to Use the Cuvette The cuvette is used for off-line measurements (no flow system required) and is basically a standard flow cell with a few changes: • wide bore capillary connections for easier injections with a syringe •...
Page 217: Flow Cell Flushing
Maintenance Flow Cell Flushing ag-textnode-version-id: 3587002635 ag-textnode-base-id: 3587002635 Flow Cell Flushing When • If flow cell is contaminated Tools required Qty. Description Glass syringe Adapter Parts required Qty. Description Bidistilled water, nitric acid (65 %), tubings to waste Dangerous concentration of nitric acid WARNING ag-struc-frag-safety-version-id: 3587848971 ag-struc-frag-safety-base-id: 3587848971...
Page 218: Correcting Leaks
Maintenance Correcting Leaks ag-textnode-version-id: 9007202841766667 ag-textnode-base-id: 3587025675 Correcting Leaks Flow Cell Area [2] Leak Sensor Area [1] Figure 75: Correcting leaks When • If a leakage has occurred in the flow cell area or at the capillary connections Tools required Qty. Description Tissue Wrench, 1/4 inch for capillary connections...
Page 219 Maintenance Correcting Leaks 1 Open the doors. 2 Use tissue to dry the leak sensor area [1]. 3 Observe the capillary connections and the flow cell area [2] for leaks and correct, if required. 4 Close the doors of the module. Fluorescence Detectors User Manual...
Page 220: Replace Leak Handling System Parts
Maintenance Replace Leak Handling System Parts ag-textnode-version-id: 3586972171 ag-textnode-base-id: 3586972171 Replace Leak Handling System Parts Parts required Qty. Description 5043-0856 Leak Adapter 5063-6527 Tubing, Silicon Rubber, 1.2 m, ID/OD 6 mm/ 9 mm Preparations • Turn off the module. • Disconnect the power cable. •...
Page 221 Maintenance Replace Leak Handling System Parts 4 Locate the Leak Interface Top. 5 Pull the Leak Adapter from the Top Cover and remove it together with the tubing. 6 Install the Leak Adapter by pressing it into the Main Cover. 7 Slide the Main Cover into its front position.
Page 222: Replace The Module Firmware
Maintenance Replace the Module Firmware ag-textnode-version-id: 9007202256802699 ag-textnode-base-id: 3002061707 Replace the Module Firmware When Install a newer firmware • It fixes known problems of older versions, or • It introduces new features, or • It ensures keeping all systems at the same (validated) revision When Install an older firmware •...
Page 223: Tests & Calibrations
Maintenance Tests & Calibrations ag-textnode-version-id: 3586975883 ag-textnode-base-id: 3586975883 Tests & Calibrations The following tests are required after maintenance of lamps and flow cells: • Lamp Intensity Test on page 137 • Wavelength Verification and Calibration on page 147 Fluorescence Detectors User Manual...
Page 224: Parts And Materials For Maintenance
ag-textnode-version-id: 9007203333153419 ag-textnode-base-id: 4078412427 Parts and Materials for Maintenance This chapter provides information on parts for maintenance. Overview of Maintenance Parts 225 Kits 226 Accessory Kit 226 Calibration Kit 226 Capillary Kit Flow Cells BIO 227 Cuvette Kit 227 Fluorescence Detectors User Manual...
Page 225: Overview Of Maintenance Parts
Parts and Materials for Maintenance Overview of Maintenance Parts ag-textnode-version-id: 9007202841868299 ag-textnode-base-id: 3587127307 Overview of Maintenance Parts Figure 76: Overview of maintenance parts Description G1321-60005 Flow cell, 8 µL, 20 bar, OR G1321-60015 Flow cell, 4 µL, 20 bar, requires a 0.12 mm i.d. capillary (e.g. p/n G1316-87318, 300 mm long), part of Capillary kit for 0.12 mm id (p/n G1316-68716), OR G5615-60005 Bio-inert flow cell, 8 µL, 20 bar, includes Capillary Kit Flow...
Page 226: Kits
Parts and Materials for Maintenance Kits ag-textnode-version-id: 3584732427 ag-textnode-base-id: 3584732427 Kits ag-textnode-version-id: 3587154187 ag-textnode-base-id: 3587154187 Accessory Kit G7121-68755 (Detector Accessory Kit) Description 5062-2462 Tube PTFE 0.7 mm x 5 m, 1.6 mm od re-order 5 m 0100-1516 Finger-tight fitting PEEK, 2/pk G1315-87311 Capillary ST 0.17 mm x 380 mm S/S Column to detector (includes ST ferrule front, ST ferrule back and ST fitting) 5063-6527...
Page 227: Capillary Kit Flow Cells Bio
Parts and Materials for Maintenance Kits ag-textnode-version-id: 3587149707 ag-textnode-base-id: 3587149707 Capillary Kit Flow Cells BIO G5615-68755 (Capillary Kit Flow Cells BIO includes Capillary PK 0.18 mm x 1.5 m and PEEK Fittings 10/PK (p/n 5063-6591)) includes: Description 0890-1763 Capillary PEEK 0.18 mm x 1.5 m 5063-6591 PEEK Fittings 10/PK ag-textnode-version-id: 3587145227...
Page 228: Identifying Cables
ag-textnode-version-id: 27021601743836299 ag-textnode-base-id: 3979613323 Identifying Cables This chapter provides information on cables used with the modules. Cable Overview 229 Analog Cables 231 Remote Cables 233 BCD Cables 237 CAN/LAN Cables 239 RS-232 Cables 240 USB 241 Fluorescence Detectors User Manual...
Page 229: Cable Overview
Identifying Cables Cable Overview ag-textnode-version-id: 9007202256869899 ag-textnode-base-id: 3002128907 Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure NOTE proper functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent 35900A A/D converter...
Page 230 Identifying Cables Cable Overview RS-232 cables Description RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It is also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.
Page 231: Analog Cables
Identifying Cables Analog Cables ag-textnode-version-id: 9007202256873995 ag-textnode-base-id: 3002133003 Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made. Agilent Module to 35900 A/D converters p/n 35900-60750 35900 Pin Agilent...
Page 232 Identifying Cables Analog Cables Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog + Fluorescence Detectors User Manual...
Page 233: Remote Cables
Identifying Cables Remote Cables ag-textnode-version-id: 9007202256882571 ag-textnode-base-id: 3002141579 Remote Cables ERI (Enhanced Remote Interface) • 5188-8029 ERI to general purpose (D-Sub 15 pin male - open end) • 5188-8044 ERI to ERI (D_Sub 15 pin male - male) • 5188-8059 ERI-Extension-Cable 1.2 m (D-Sub15 pin male / female) p/n 5188-8029 Color code Enhanced Remote Classic Remote...
Page 234 Identifying Cables Remote Cables • 5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG)) p/n 5188-8045 Pin (ERI) Signal Pin (APG) Active (TTL) Start Request Stop Ready High Power on High Future Shut Down Start Prepare Ground Cable Shielding Fluorescence Detectors User Manual...
Page 235 High Ground Cable Shielding One end of these cables provides an Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Fluorescence Detectors User Manual...
Page 236 Identifying Cables Remote Cables Agilent Module to Agilent 35900 A/D Converters p/n 5061-3378 Pin 35900 A/D Pin Agilent Signal Name Active module (TTL) 1 - White 1 - White Digital ground 2 - Brown 2 - Brown Prepare run 3 - Gray 3 - Gray Start 4 - Blue 4 - Blue Shut down 5 - Pink...
Page 237: Bcd Cables
Identifying Cables BCD Cables ag-textnode-version-id: 3358242315 ag-textnode-base-id: 3358242315 BCD Cables One end of these cables provides a 15-pin BCD connector to be connected to the Agilent modules. The other end depends on the instrument to be connected to Agilent Module to General Purpose p/n G1351-81600 Wire Color Pin Agilent...
Page 238 Identifying Cables BCD Cables Agilent Module to 3396 Integrators p/n 03396-60560 Pin 3396 Pin Agilent Signal Name BCD Digit module BCD 5 BCD 7 BCD 6 BCD 4 BCD0 BCD 3 BCD 2 BCD 1 Digital ground + 5 V Fluorescence Detectors User Manual...
Page 239: Can/Lan Cables
Identifying Cables CAN/LAN Cables ag-textnode-version-id: 18014401511606795 ag-textnode-base-id: 3002124811 CAN/LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors. Can Cables Description 5181-1516 CAN cable, Agilent module to module, 0.5 m 5181-1519 CAN cable, Agilent module to module, 1 m LAN Cables Description...
Page 240: Rs-232 Cables
Identifying Cables RS-232 Cables ag-textnode-version-id: 9007202256878091 ag-textnode-base-id: 3002137099 RS-232 Cables Description RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It is also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.
Page 241: Usb
Identifying Cables ag-textnode-version-id: 9007202256886667 ag-textnode-base-id: 3002145675 To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Fluorescence Detectors User Manual...
Page 242: Hardware Information
ag-textnode-version-id: 9007205354304139 ag-textnode-base-id: 6099563147 Hardware Information This chapter describes the module in more detail on hardware and electronics. General Hardware Information 243 Firmware Description 243 Electrical Connections 245 Interfaces 247 Instrument Layout 254 Early Maintenance Feedback (EMF) 255 Module-Specific Hardware Information 256 Setting the 6-bit Configuration Switch 256 Fluorescence Detectors User Manual...
Page 243: General Hardware Information
Hardware Information General Hardware Information ag-textnode-version-id: 36028801083399691 ag-textnode-base-id: 4064435723 General Hardware Information This section provides detailed hardware information on firmware that is valid for this module. ag-textnode-version-id: 3002159627 ag-textnode-base-id: 3002159627 Firmware Description The firmware of the instrument consists of two independent sections: •...
Page 244: Firmware Updates
Hardware Information General Hardware Information • or module specific functions like • internal events such as lamp control, filter movements, • raw data collection and conversion to absorbance. Firmware Updates Firmware updates can be done with the Agilent Lab Advisor software with files on the hard disk (latest version should be used).
Page 245: Electrical Connections
One analog output provides signals for integrators or data handling systems. • The ERI connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start, stop, common shut down, prepare, and so on. Fluorescence Detectors User Manual...
Page 246: Rear View Of The Module
— Any other instruments connected to this instrument shall be approved to a suitable safety standard and must include reinforced insulation from the mains. Never use cables other than the ones supplied by Agilent Technologies to ensure NOTE proper functionality and compliance with safety or EMC regulations.
Page 247: Serial Number Information
(there can be more than one code for the same module) 00000 Serial number ag-textnode-version-id: 18014402069660043 ag-textnode-base-id: 3560178059 Interfaces The Agilent InfinityLab LC Series modules provide the following interfaces: Table 31: Agilent InfinityLab LC Series interfaces Module RS-232 Analog APG (A) / Special...
Page 248 Hardware Information General Hardware Information Module RS-232 Analog APG (A) / Special (on-board) ERI (E) G7157A Detectors G7114A/B G7115A G7117A/B/C G7121A/B G7162A/B G7165A Fraction Collectors G7158B G7159B G7166A Requires a host module with on-board LAN with minimum FW B.06.40 or C.06.40, or with additional G1369C LAN Card G1364E/F, G5664B...
Page 249: Overview Interfaces
Hardware Information General Hardware Information LAN connection is made between at least one of the Agilent modules and the NOTE Control PC. — If an Assist Hub is installed, connect the LAN to the Lab LAN port of this module. —...
Page 250: Analog Signal Output
Remote (ERI) The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
Page 251: Special Interfaces
Hardware Information General Hardware Information for next analysis, followed by START of run and optional STOP of run triggered on the respective lines. In addition PREPARE and START REQUEST may be issued. The signal levels are defined as: • standard TTL levels (0 V is logic true, + 5.0 V is false), •...
Page 252 ERI replaces the AGP Remote Interface that is used in the HP 1090/1040/1050/1100 HPLC systems and Agilent 1100/1200/1200 Infinity HPLC modules. All new InfinityLab LC Series products using the communication board core electronics use ERI. This interface is already used in the Agilent Universal Interface Box 2 (UIB2)
Page 253 Hardware Information General Hardware Information Enhanced Remote PGND +24 V ERI out +24 V ERI out IO (Input/Output) Lines • Eight generic bi-directional channels (input or output). • Same as the APG Remote. • Devices like valves, relays, ADCs, DACs, controllers can be supported/ controlled.
Page 254: Instrument Layout
Hardware Information General Hardware Information • If a connected device requires more power the firmware detects this (overcurrent detection) and provides the information to the user interface. • Fuse used for safety protection (on board). • Short circuit will be detected through hardware. ag-textnode-version-id: 3002198795 ag-textnode-base-id: 3002198795 USB (Universal Serial Bus)
Page 255: Early Maintenance Feedback (Emf)
Hardware Information General Hardware Information ag-textnode-version-id: 3002155147 ag-textnode-base-id: 3002155147 Early Maintenance Feedback (EMF) Maintenance requires the exchange of components that are subject to wear or stress. Ideally, the frequency at which components are exchanged should be based on the intensity of use of the module and the analytical conditions, and not on a predefined time interval.
Page 256: Module-Specific Hardware Information
Hardware Information Module-Specific Hardware Information ag-textnode-version-id: 9007203333355019 ag-textnode-base-id: 4078614027 Module-Specific Hardware Information ag-textnode-version-id: 18014402036253963 ag-textnode-base-id: 3526771979 Setting the 6-bit Configuration Switch The 6-bit configuration switch is located at the rear of the module with communication board electronics. Switch settings provide configuration parameters for LAN and instrument specific initialization procedures.
Page 257 Hardware Information Module-Specific Hardware Information Mode Init Mode Test Boot Main System/Revert to Default Data Test Boot Resident System/Revert to Default Data Legend: 0 (switch down), 1 (switch up), SW (switch) ag-textnode-version-id: 3285054731 ag-textnode-base-id: 3285054731 Special Settings Boot-Resident/Main Firmware update procedures may require this mode in case of firmware loading errors (main/resident firmware part).
Page 258 Hardware Information Module-Specific Hardware Information Loss of data CAUTION ag-struc-frag-safety-version-id: 3182477195 ag-struc-frag-safety-base-id: 3182477195 Forced cold start erases all methods and data stored in the non-volatile memory. Exceptions are calibration settings, diagnosis and repair log books which will not be erased. —...
Page 259: Appendix
Safety Symbols 265 Material Information 268 Materials Used in the Bio-inert LC System 268 General Information About Solvent/Material Compatibility 270 At-a-Glance Details About Agilent Capillaries 276 Waste Electrical and Electronic Equipment (WEEE) Directive 280 Radio Interference 281 Sound Emission 282 Agilent Technologies on Internet 283 Fluorescence Detectors User Manual...
Page 260: General Safety Information
Appendix General Safety Information ag-textnode-version-id: 63050397785402635 ag-textnode-base-id: 3002215691 General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument.
Page 261: Before Applying Power
— Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument. — Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
Page 262: Do Not Operate In An Explosive Atmosphere
Appendix General Safety Information ag-textnode-version-id: 3002235275 ag-textnode-base-id: 3002235275 Do Not Operate in an Explosive Atmosphere Presence of flammable gases or fumes WARNING ag-struc-frag-safety-version-id: 3004420107 ag-struc-frag-safety-base-id: 3004420107 Explosion hazard — Do not operate the instrument in the presence of flammable gases or fumes.
Page 263: Solvent Information
Appendix General Safety Information ag-textnode-version-id: 18014402035996427 ag-textnode-base-id: 3526514443 Solvent Information Toxic, flammable and hazardous solvents, samples and reagents WARNING ag-struc-frag-safety-version-id: 3004430475-2 ag-struc-frag-safety-base-id: 3004430475 The handling of solvents, samples and reagents can hold health and safety risks. — When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
Page 264 Appendix General Safety Information Recommendations on the Use of Solvents Observe the following recommendations on the use of solvents. • Brown glass ware can avoid growth of algae. • Follow the recommendations for avoiding the growth of algae, see the pump manuals.
Page 265: Magnets
Appendix General Safety Information ag-textnode-version-id: 3285065867 ag-textnode-base-id: 3285065867 Flow cell To protect optimal functionality of your flow-cell: • Avoid the use of alkaline solutions (pH > 9.5) which can attack quartz and thus impair the optical properties of the flow cell. ag-textnode-version-id: 27021600810554507 ag-textnode-base-id: 3046331531 Magnets Figure 81: Magnets in doors of pumps, autosamplers, detectors, and fraction collectors...
Page 266 Appendix General Safety Information The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up. Indicates flammable material used. Consult the Agilent Information Center / User Manual before attempting to install or service this equipment. Follow all safety precautions.
Page 267 Appendix General Safety Information A WARNING WARNING ag-struc-frag-safety-version-id: 3004433931 ag-struc-frag-safety-base-id: 3004433931 alerts you to situations that could cause physical injury or death. — Do not proceed beyond a warning until you have fully understood and met the indicated conditions. A CAUTION CAUTION ag-struc-frag-safety-version-id: 3004437387 ag-struc-frag-safety-base-id: 3004437387...
Page 268: Material Information
3358303627 Materials Used in the Bio-inert LC System For the Bio-inert LC system, Agilent Technologies uses highest-quality materials in the flow path (also referred to as wetted parts), which are widely accepted by life science scientists, as they are known for optimum inertness to biological samples and ensure best compatibility with common samples and solvents over a wide pH range.
Page 269 Appendix Material Information Table 35: Used bio-inert materials Module Materials Agilent 1260 Infinity III Bio-inert Pump Titanium, gold, platinum-iridium, ceramic, (G5654A) ruby, PTFE, PEEK Agilent 1260 Infinity III Bio-inert Multisampler Upstream of sample introduction: (G5668A) • Titanium, gold, PTFE, PEEK, ceramic Downstream of sample introduction: •...
Page 270: General Information About Solvent/Material Compatibility
Appendix Material Information ag-textnode-version-id: 3002994315 ag-textnode-base-id: 3002994315 General Information About Solvent/Material Compatibility Materials in the flow path are carefully selected based on Agilent’s experiences in developing highest-quality instruments for HPLC analysis over several decades. These materials exhibit excellent robustness under typical HPLC conditions. For any special condition, please consult the material information section or contact Agilent.
Page 271: Polyphenylene Sulfide (Pps)
Appendix Material Information Polyphenylene Sulfide (PPS) Polyphenylene sulfide has outstanding stability even at elevated temperatures. It is resistant to dilute solutions of most inorganic acids, but it can be attacked by some organic compounds and oxidizing reagents. Nonoxidizing inorganic acids, such as sulfuric acid and phosphoric acid, have little effect on polyphenylene sulfide, but at high concentrations and temperatures, they can still cause material damage.
Page 272 Appendix Material Information Polyimide Agilent uses semi-crystalline polyimide for rotor seals in valves and needle seats in autosamplers. One supplier of polyimide is DuPont, which brands polyimide as Vespel, which is also used by Agilent. Polyimide is stable in a pH range between 1 and 10 and in most organic solvents. It is incompatible with concentrated mineral acids (e.g.
Page 273 Appendix Material Information • Halogenated solvents or mixtures, which form radicals and/or acids, for example: 2 CHCl → 2 COCl + 2 HCl This reaction, in which stainless steel probably acts as a catalyst, occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol.
Page 274 Appendix Material Information Fused Silica and Quartz (SiO Fused silica is used in Max Light Cartridges. Quartz is used for classical flow cell windows. It is inert against all common solvents and acids except hydrofluoric acid and acidic solvents containing fluorides. It is corroded by strong bases and should not be used above pH 12 at room temperature.
Page 275 Appendix Material Information The tubing of the leak sensor is made of PVDF (polyvinylidene fluoride), which is incompatible with the solvent DMF (dimethylformamide). Sapphire, Ruby, and Al -Based Ceramics Sapphire, ruby, and ceramics based on aluminum oxide Al are inert to almost all common acids, bases, and solvents.
Page 276: At-A-Glance Details About Agilent Capillaries
Appendix At-a-Glance Details About Agilent Capillaries ag-textnode-version-id: 18014401512496395 ag-textnode-base-id: 3003014411 At-a-Glance Details About Agilent Capillaries The following section provides useful information about Agilent capillaries and its characteristics. Syntax for capillary description Type - Material - Capillary dimensions - Fitting Left/Fitting right Table 36: Example for a capillary description Code provided Meaing of the code...
Page 277 Appendix At-a-Glance Details About Agilent Capillaries Color Coding Guide Table 37: Color-coding key for Agilent capillary tubing Internal diameter in mm Color code 0.015 Orange 0.025 Yellow 0.05 Beige 0.075 Black 0.075 MP35N Black with orange stripe 0.1 Purple 0.12 0.12 MP35N Red with orange stripe 0.17 ...
Page 278 Appendix At-a-Glance Details About Agilent Capillaries Description Tube Tubing Heat exchanger Heat exchanger Abbreviation Guide for Material Table 39: Material (indicates which raw material is used for the capillary) Description Stainless steel Titanium PEEK FS/PK PEEK-coated fused silica PK/ST Stainless steel-coated PEEK PFFE PTFE Fused silica...
Page 279 Appendix At-a-Glance Details About Agilent Capillaries Abbreviation Guide for Fitting Left/Fitting Right Table 41: Fitting left/fitting right (indicates which fitting is used on both ends of the capillary) Description Swagelok + 0.8 mm Port id Swagelok + 1.6 mm Port id Metric M4 + 0.8 mm Port id Metric M3 + 1.6 mm Port id Swagelok union Long...
Page 280: Waste Electrical And Electronic Equipment (Weee) Directive
Appendix Waste Electrical and Electronic Equipment (WEEE) Directive ag-textnode-version-id: 3002258059 ag-textnode-base-id: 3002258059 Waste Electrical and Electronic Equipment (WEEE) Directive This product complies with the European WEEE Directive marking requirements. The affixed label indicates that you must not discard this electrical/electronic product in domestic household waste.
Page 281: Radio Interference
Radio Interference ag-textnode-version-id: 3002261771 ag-textnode-base-id: 3002261771 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with unscreened cables, or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises.
Page 282: Sound Emission
Appendix Sound Emission ag-textnode-version-id: 3002271243 ag-textnode-base-id: 3002271243 Sound Emission Sound Pressure Sound pressure Lp < 70 db(A) according to DIN EN ISO 7779 Schalldruckpegel Schalldruckpegel Lp < 70 db(A) nach DIN EN ISO 7779 Fluorescence Detectors User Manual...
Page 283: Agilent Technologies On Internet
Appendix Agilent Technologies on Internet ag-textnode-version-id: 3002266251 ag-textnode-base-id: 3002266251 Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: https://www.agilent.com Fluorescence Detectors User Manual...
Page 284 The manual describes the following: • introduction and specifications, • using and optimizing, • troubleshooting and diagnose, • maintenance, • parts identification, • hardware information, • safety and related information. www.agilent.com © Agilent Technologies Inc. 2016-2024 Edition: 10/2024 Document No: SD-29000222 Rev. D...

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