Priming The System - Agilent Technologies Agilent 1260 Infinity System User's Manual

Binary lc
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4
System Setup and Installation
Installing the Modules

Priming the System

Initial Priming
When
Before a degasser or solvent tubing can be used, it is necessary to prime the system. Isopropanol is
recommended as priming solvent due to its miscibility with nearly all HPLC solvents and its
excellent wetting properties.
Parts required
#
1
Preparations
Connect all modules hydraulically as described in the respective module manuals.
Fill each solvent bottle with 100 mL isopropanol
Switch the system on
When opening capillary or tube fittings, solvents may leak out.
WA R N I N G
The handling of toxic and hazardous solvents and reagents can carry health risks.
The purge tool of the Lab Advisor can be used to purge the pump automatically.
N O T E
If the pump is not able to draw in the solvent from the bottles, use a syringe to move the
N O T E
solvent manually through tubing and degasser.
When priming the vacuum degasser with a syringe, the solvent is drawn through the
N O T E
degasser tubes very quickly. The solvent at the degasser outlet will therefore not be fully
degassed. Pump for approximately 10 minutes at your desired flow rate before starting an
analysis. This will allow the vacuum degasser to properly degas the solvent in the degasser
tubes.
76
Description
Isopropanol
Observe appropriate safety procedures (for example, wear goggles, safety gloves
and protective clothing) as described in the material handling and safety data sheet
supplied by the solvent vendor, especially when toxic or hazardous solvents are
used.
1260 Infinity Binary LC - System User Guide

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