Agilent Technologies Agilent 1260 Infinity System User's Manual page 126

6
Appendix
Setting Up a Method using Edit Entire Method
• Advanced - Spectrum
126
Spectra can be saved during the run on a continuous or peak- controlled
basis (this applies to the ChemStation software. Some software
packages, e.g. EZChrom, only support continuous collection of all
spectra and the peak- controlled options do not appear). Spectral
collection and signal collection are independent operations that are
performed by the detector firmware and are not dependent on the
computer software extracting data from the 3D data matrix. The rate at
which spectra are taken is determined by the Peakwidth setting and
eight spectra are taken during the time that is specified in Peakwidth.
The firmware does peak detection on signal A only to determine when
peak- controlled spectra should be saved. For multiple signals, it may be
necessary to have signal A set as a broadband detector to ensure that
peak spectra are available for all the different wavelength signals.
• Store controls the spectral collection mode with the following options:
None – no spectra are stored,
Apex+Baselines – three spectra are taken at the start, apex, and end
of peak,
Apex+Slopes+Baselines – five spectra are taken at the start, upslope,
apex, downslope, and end of peak,
All in Peak – all the available spectra within a peak are stored,
All – all spectra throughout the run are stored,
Every 2nd Spectrum – stores only alternate spectra that are acquired
throughout the run.
• Range – Spectra can be saved across the full range of the detector,
190 nmto 640 nm, or any reduced range that is chosen as
appropriate by the user. (This also reduces the number of data to be
stored).
• Step– controls the interval (nm) of the data that are stored in a
spectrum and hence affects the spectral resolution seen. The default
setting of 2 nm is a good choice for most applications.
• Threshold – determines that spectra are not stored for peak heights
below this value (mAU).
1260 Infinity Binary LC - System User Guide