Agilent Technologies Agilent 1260 Infinity System User's Manual page 15

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The Agilent 1260 Infinity Binary LC - Product Description
System Components
1260 Infinity High Performance Autosampler (G1367E)
Features
The 1260 Infinity High Performance Autosampler features an increased
pressure range (up to 600 bar) enabling the use of today's column
technology (sub- two- micron narrow bore columns) with the Agilent 1260
Infinity Binary LC. Increased robustness is achieved by optimized new parts,
high speed with lowest carry- over by flow through design, increased sample
injection speed for high sample throughput, increased productivity by using
overlapped injection mode, and flexible and convenient sample handling with
different types of sample containers, such as vials and well plates. Using
384- well plates allows you to process up to 768 samples unattended.
Autosampler Principle
The movements of the autosampler components during the sampling
sequence are monitored continuously by the autosampler processor. The
processor defines specific time windows and mechanical ranges for each
movement. If a specific step of the sampling sequence is not completed
successfully, an error message is generated. Solvent is bypassed from the
autosampler by the injection valve during the sampling sequence. The
needle moves to the desired sample position and is lowered into the
sample liquid in the sample to allow the metering device to draw up the
desired volume by moving its plunger back a certain distance. The needle
is then raised again and moved onto the seat to close the sample loop.
Sample is applied to the column when the injection valve returns to the
mainpass position at the end of the sampling sequence.
The standard sampling sequence occurs in the following order:
1 The injection valve switches to the bypass position.
2 The plunger of the metering device moves to the initialization position.
3 The needle lock moves up.
4 The needle moves to the desired sample vial (or well plate) position.
5 The needle lowers into the sample vial (or well plate).
6 The metering device draws the preset sample volume.
7 The needle lifts out of the sample vial (or well plate).
8 The needle is then moved onto the seat to close the sample loop.
9 The needle lock moves down.
10 The injection cycle is completed when the injection valve switches to
the mainpass position.
If needle wash is required it will be done between step 7 and 8.
1260 Infinity Binary LC - System User Guide 15