Agilent Technologies Agilent 1260 Infinity System User's Manual page 57

Extra-column Volume
To maintain the high resolution achieved on the column, the extra- column
volume, especially after the column, should be as low as possible.
• For 4.6 mm inner diameter columns the standard delay configuration
• For 2.1 mm inner diameter columns, the low delay configuration should
• The injection volume is also of importance, especially if the sample is
• The 10 mm Max- Light cartridge cell has a low dispersion volume (σ
• In situations where the alternative 60 mm Max- Light high sensitivity
1260 Infinity Binary LC - System User Guide
should be used for the pump, see
be used for the pump, and the low dispersion kit should be installed in
the column compartment. For highest UV sensitivity, the short mixer is
also recommended. See
page 44.
dissolved in an organic solvent. In this case, the gradient should start
with a low percentage of organic phase to focus the compounds at the
top of the column. This avoids peaks dispersion due to the injection.
volume 1.0 µL) and no further volume optimization is required.
cell is used to get higher sensitivity the cell volume is optimized for the
use with 3 mm and 4.6 mm inner diameter columns.
Optimization of the Agilent 1260 Infinity Binary LC
How to Achieve Higher Resolution
Figure 15
Figure 13 on page 43 and
on page 45.
Figure 14 on
3
57