Agilent Technologies Agilent 1260 Infinity System User's Manual page 79

Changing Solvents
When When the solvent of a channel is to be replaced by another solvent that is not compatible (solvents
are immiscible or one solvent contains a buffer), it is necessary to follow the procedure below to
prevent clogging of the pump by salt precipitation or residual liquid droplets in parts of the system.
Parts required #
1
1
Preparations Remove the column and replace it by a ZDV fitting
Prepare bottles with appropriate intermediate solvents (see
1 If the channel is not filled with buffer, proceed to step 4.
2 Place the solvent intake filter into a bottle of water.
3 Flush the channel at a flow rate suitable for the installed tubing
4 Modify the flow path of your system as required for your application.
Buffer salt of aqueous buffers may precipitate in residual isopropanol.
C A U T I O N
Capillaries and filter may be clogged by precipitating salt.
➔
➔
5 Replace the solvent bottle by a bottle of isopropanol.
6 Flush the channel at a flow rate suitable for the installed tubing
7 Swap the bottle of isopropanol with a bottle of solvent for your
8 Repeat steps 1 to 7 for the other channel(s) of the pump.
9 Install the desired column, set the required composition and flow rate
1260 Infinity Binary LC - System User Guide
p/n
5022-2184
(typically 3 – 5 mL/min) for 10 min.
Flush solvent lines containing high concentration of salts first with water before
introducing organic solvent.
Do not perform steps 5 to 7 for channels running with aqueous buffer as solvent.
(typically 3 – 5 mL/min) for 5 min.
application.
for your application and equilibrate the system for approx. 10 minutes
prior to starting a run.
Description
Purging solvent(s), see Table 8
Union ZDV
System Setup and Installation
Installing the Modules
on page 80
Table 8 on page 80)
4
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