Agilent Technologies Agilent 1260 Infinity System User's Manual page 59

Table 6
Data Rate
80 Hz
40 Hz
20 Hz
10 Hz
5 Hz
80 Hz data rate compared with 20 Hz data rate results in improvements
in:
• Peak Width:
• Resolution:
• Peak capacity:
• Apparent column efficiency:
Chromatographic Conditions
As already stated previously, chromatographic conditions depend on the
compounds to be analyzed. But here also, some rules of thumb exist.
• Moderate flow rates should be used, but recent experiments have shown
• Moderate gradients should be used, for example, 2 – 5 % gradient
• The column temperature is an additional parameter for optimization.
1260 Infinity Binary LC - System User Guide
Relation between data rate and chromatographic performance
Peak Width
0.300
0.329
0.416
0.666
1.236
– 30 %
+ 30 %
+ 40 %
+ 70 %
that elevated flow rates can also be advantageous for improved
separation. For 4.6 mm inner diameter columns packed with
sub- 2- micron particles, a flow rate of 2 mL/min is recommended as a
starting value. For 2.1 mm inner diameter sub- 2- micron columns,
0.4 mL/min is a good starting point.
change per minute.
Temperature can influence a separation, and should not be overlooked.
Optimization of the Agilent 1260 Infinity Binary LC
How to Achieve Higher Resolution
Resolution
2.25
2.05
1.71
1.17
0.67
3
Peak Capacity
60
55
45
29
16
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