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3
Optimization of the Agilent 1260 Infinity Binary LC
How to Configure the Optimum Delay Volume
Optimum Instrument Configuration for 2.1 mm i.d. Columns
Low delay volume configuration to achieve shortest gradient delay for
ultra-fast gradient separations
In the low delay volume configuration of the Agilent 1260 Infinity Binary
Pump the damper and mixer are bypassed to reduce the pump delay
volume to about 120 µL.
Figure 13
on page 43shows the flow path
connections for this configuration. This provides the shortest gradient
delay for ultra- fast gradient separations. In order to take full advantage of
the electronic damping control which replaces the physical volume
damping it is important to select the respective Enhanced Solvent
Compressibility function in the auxiliary screen of the pump menu.
To minimize peak dispersion the low dispersion kit ( Low dispersion kit
(G1316- 68744)) must be installed. This kit includes short 0.12 mm i.d.
capillaries and low dispersion heat exchangers (1.6 µL and 1.5 µL) for the
thermostatted column compartment. To maintain resolution in the UV
detector, a low volume flow cell should be used. See
"Choosing a Flow
Cell"
on page 63 for flow cell recommendations).
Figure 13
Low delay configuration for 2.1 mm inner diameter columns
It is important to remember to set the correct parameter in the pump
auxiliary screen. This ensures that the correct compressibility values are
always applied for the mobile phases used. Calibration curves are available
for most common solvents.
1260 Infinity Binary LC - System User Guide
43
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