Agilent Technologies Agilent 1260 Infinity System User's Manual page 121

• Show timetable graph displays the timetable changes graphically when the
• Stop Timedefines the overall time for the separation or run and is
• Post Time defines a countdown period after the end of a run during which
• Pressure Limits control the behavior of the pump regarding pressure. The
1260 Infinity Binary LC - System User Guide
the settings for time 0.00 min into the timetable, these values are picked
up from other setpoints on this screen. However, some users like to see a
'complete' list in the timetable and make an entry for 0.00 min. There is
no problem with this but if the initial conditions are ever changed then
the new settings must be entered in both the timetable and the setpoints
in the Solvents section of the screen.
box is ticked.
sometimes referred to as the 'Run Time' by some users. This is the time
(counted in minutes since the injection was made) when the run ends,
which means that data acquisition will stop, the flow, composition, and
other system settings will revert to the initial values for the method and
the system will become available to make the next injection. It should
always be at least as long as the last entry in the timetable or else the
run will stop and revert to starting conditions before the timetable events
are complete. Stop Time can be set to No Limit in which case the user must
manually stop the run. While all the system modules have Stop Time
parameters, the Stop Time in the pump is considered to be the master and
the other modules will usually be set to follow this value.
the next injection is prevented. It allows time for the system to
re- equilibrate after a gradient run. For an isocratic method it can be set
to Off. For a gradient method, the value can be determined experimentally
by observing the baseline behavior but typically will need time for the
delay volume of the system plus at least three to five column volumes to
be flushed through the system.
maximum pressure of the 1260 Infinity Binary Pump is 600 bar but some
columns can only withstand lower pressure and by setting this value here
the column will be protected. The pump will generate an error condition
if this pressure is exceeded. Any run in progress will be stopped and the
pump switched to standby mode with no flow. Information about the
maximum pressure for a particular column is supplied with the column.
Agilent ZORBAX RRHT columns are suitable for operation at 600 bar. The
Low Pressure limit is 'Off' when the value is zero but at any other value
the pump will generate an error if the pressure goes below this value
during operation. This is used as an alternative safeguard when the
column is not inside a module with a leak sensor or in case the system is
pumped dry. A value of 10 to 20 bar for minimum pressure limit is
typical.
Setting Up a Method using Edit Entire Method
6
Appendix
121