Agilent Technologies Agilent 1260 Infinity System User's Manual page 91

Checkout Preparation
1 Fill solvent bottle A with HPLC grade Water. Fill solvent bottle B with
2 If installed, purge each channel of the on- line degasser using the
3 Open the purge valve of the pump, and purge each channel at
4 Install the supplied column into the column compartment.
5 Flush the flow- cell with HPLC- grade water for 5 min.
6 Run a Wavelength Calibration and Intensity Test where applicable.
7 Allow the system to equilibrate at the checkout conditions listed in
8 Prepare a vial of the isocratic checkout sample.
1260 Infinity Binary LC - System User Guide
HPLC grade Acetonitrile.
supplied syringe.
5 mL/min.
This should be sufficient to remove air in the system.
Table 9 on page 89 and
Data Acquisition in Method and Run Control View
Table 10 on page 90 for 10 min.
5
Quick Start Guide
91