Agilent Technologies Agilent 1260 Infinity System User's Manual page 122

Binary lc
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6
Appendix
Setting Up a Method using Edit Entire Method
Thermostatted Column Compartment (TCC) tab
Figure 28
• Temperature defines the temperature of the left and right- hand side
122
Setup Method screen – Thermostatted Column Compartment tab
column holders which can be independently controlled or linked
together by clicking the Combined radio button on. When combined the
settings for the left side control both sections and this is certainly
necessary when the column is longer than 15 cm and needs to be
supported by both sections. The two sides can be operated separately
when it is required to have two columns operating at different
temperatures – this can be implemented when a column switching valve
is also installed to switch between them. Another use of separate
temperature zones is when the column is operated at a high
temperature (e.g. above 60 °C) on one side and the heat exchanger on
the other side is used to cool the eluent before it enters the detector
thereby reducing noise due to thermal effects in the flow cell. Selecting
the As Detector Cell option will automatically read the temperature from
the cell in the detector.
1260 Infinity Binary LC - System User Guide

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