Agilent Technologies Agilent 1260 Infinity System User's Manual page 115

Autosampler tab
Figure 25
• Injection Mode
• Needle Wash – if selected above.
Vials should not have a septum i.e., they should be open to avoid transfer of carryover
N O T E
material on the septum.
1260 Infinity Binary LC - System User Guide
Setup Method screen – High Performance Autosampler tab
• Injection volume sets the volume to be injected (example 1 µl),
• Standard injection indicates that no external needle wash is done,
• Injection with needle wash is used to reduce potential carryover. This is
the recommended option and is configured in the next entry.
• Mode determines how the outside of the needle will be rinsed: either
actively in the Flush Port or by dipping into a specified Wash Vial.
• Time in seconds the peristaltic pump connected to the flush port will
pump the rinse solvent. It then pumps for a further 15 s to clear the
flush port.
• Location determines which vial or well plate will be used if Wash Vial
was selected.
Setting Up a Method using Edit Entire Method
6
Appendix
115