Optimum Instrument Configuration For High Resolution - Agilent Technologies Agilent 1260 Infinity System User's Manual

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Optimization of the Agilent 1260 Infinity Binary LC
How to Achieve Higher Resolution

Optimum Instrument Configuration for High Resolution

Column Compartment
The column compartment can be used in its standard version for 4.6 mm
inner diameter columns. At flow rates above 2 mL/min, and temperatures
above 60 °C, the column effluent should be cooled down to the
temperature of the detector by using the 1.5 µL cooler/heater in the
column compartment. This ensures lowest noise level with UV detectors,
even at 5 mL/min and 80 °C (see
Figure 18
If 2.1 mm inner diameter columns are used at low flow rates, the small
heating device should be used for lowest extra- column volume.
56
Influence of post column cooling (PCC) on baseline noise
Figure 18 on page 56 ).
1260 Infinity Binary LC - System User Guide