Agilent Technologies Agilent 1260 Infinity System User's Manual page 32

2
Introduction
Theory of Using Smaller Particles in Liquid Chromatography
Resolution can be described as a function of three parameters:
• column efficiency or theoretical plates (N),
• selectivity (α),
• retention factor (k).
According to the resolution equation (
selectivity has the biggest impact on resolution
This means that the selection of appropriate mobile and stationary phase
properties and temperature is critical in achieving a successful separation.
Figure 9
Figure 10
32
Resolution equation
Effect of plate number, separation factor and retention factor on R
Figure 9 on page 32 ), the
(Figure 10
1260 Infinity Binary LC - System User Guide
on page 32).