Setting Up The Method - Agilent Technologies Agilent 1260 Infinity System User's Manual

5
Quick Start Guide
Data Acquisition in Method and Run Control View

Setting Up the Method

analysis.
The default method DEF_LC.M has been loaded ready to prepare the new method. Now the
Prerequisites
key parameters can be edited to create the new method.
1 To quickly access the Method page for each module, right- click in the
2 Right- click the pump area, and select Method... in the context menu.
3 Right- click the autosampler area, and select Method... in the context
92
This section shows how to quickly set the method conditions for an
system diagram for the module and select Method... from the context
menu.
Each of the modules will be set up in this way.
a In the Method page for the 1260 Infinity Binary Pump, enter the following
parameters:
•
Flow rate: 4.0 ml/min
• Solvent A: Select Water from the compressibility drop- down list.
•
Solvent B: Select the check box to make Solvent B active.
•
Set the value for %B to 60 %
•
Stop Time: 1 min
•
Post Time: 1 min
b Other parameters can remain at their default settings. Click OK to
exit the window.
The changes are sent to the pump module.
menu.
a In the Method page for the 1260 Infinity Autosampler, enter the following
parameters:
•
Injection volume: 1.0 µl
•
Injection with Needle Wash
•
Mode Flush Port, Time: 6 s
b Other parameters can remain at their default settings. Click OK to
exit the window.
The changes are sent to the autosampler module.
1260 Infinity Binary LC - System User Guide