Agilent Technologies Agilent 1260 Infinity System User's Manual page 116

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Appendix
Setting Up a Method using Edit Entire Method
• Stop Time / Post Time are set to No Limit / Off and these values are taken
• Advanced - Auxiliary
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• Repeat determines, if Wash Vial was selected, how many times the
needle dips into the vial (default 3, maximum 5).
care of in the pump tab.
• Draw speed is the speed that the sample is pulled into the needle.
Default value is 100 µL/min. It should be slowed down for viscous
samples or for greater precision with small sample volumes (<2 µL).
• Eject speed is the speed of delivery from the needle.
• Draw position is the vertical offset from the nominal injection position
above the bottom of a vial. Usually a draw offset of 0 would be
sufficient to take the sample from near the bottom of the vial. With
the HiP ALS, for example, a value of 0 would place the tip of the
needle 1 mm above the bottom of a wellplate.
• Equilibration time is a delay time between drawing the sample and
moving the needle.
• Sample flush out factor determines how long the autosampler would wait
after injection before allowing the valve to turn to bypass. This
ensures that the sample zone has cleared from the needle, seat, and
injection valve. Default value is 5.
• Vial/Well bottom sensing is an alternative to using draw position offset.
The needle slowly moves down until it touches the bottom of the vial
or well and then lifts up to the preset draw offset position. This is a
versatile way of ensuring the needle is close to the bottom of the vial
but it takes slightly longer to complete the injection and should not
be used if there are particulates at the bottom of the vial which
might block the needle.
1260 Infinity Binary LC - System User Guide