Agilent Technologies Agilent 1260 Infinity System User's Manual page 125

• Peakwidth sets the data collection rate and signal filtering.
• Stoptime / Posttime are set to As Pump/Injector and Off and these values
• Timetable operates in the same way as other modules: add a line, select
1260 Infinity Binary LC - System User Guide
• Reference Wavelength sets the central wavelength (nm) of the reference
band which is subtracted from the analytical signal,
• Reference Bandwidth sets the width (nm) of the reference band.
are usually taken care of in the Pump tab. However, a detector stop time
that is different from the pump stop time can be set if it is required to
stop data analysis before the end of the run which is defined in the
pump. This can be the case when a gradient equilibration ramp has
been set at the end of the gradient. For instance, the %B may rise to
95 % at 10 min and it is expected that all peaks have eluted from the
column. The run is essentially at an end but an extra gradient segment
is added which takes %B back down to the initial value over two
minutes to gently start the re- equilibration of the column. No useful
data is expected during this downwards ramp so the detector stop time
is set to 10 min and data collection stops while the pump stop time is
12 min to allow the downwards ramp to complete. This is a matter of
user choice and some accept that the final minutes of the
chromatogram cannot contain useful data but record it anyway to avoid
the inconvenience of having a different stop time for the detector. The
detector stop time does not override the pump and cause the run to
end as would an earlier stop time in any other module hence the
convenience of setting stop time to As Pump/Injector.
the function to be changed and enter the new values for that function.
The changes for the detector will take place immediately at the
specified time. The following functions can be changed during the run:
• Balance
• Change Signal
• Change Threshold
• Change Peak –detector Peakwidth
• Change Spectra Acquisition Mode
• Change Contacts
Setting Up a Method using Edit Entire Method
6
Appendix
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