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13.C. Starting a Run
Easy-to-use protocol wizards can be accessed from the Home screen to set up
the run parameters. Follow the steps below to set up a Fluorescence run.
1. From the Home screen, touch the "Instrument Control" button, and then
the "Read" button.
2. Select and drag the "Fluorescence" icon from the Protocol Options to the
Protocol Composer.
3. Touch the "Plate Format" button and select the plate format that is
required.
4. Touch the "Plate" button and select the wells to be read. Green indicates a
well is selected and gray indicates a well is deselected.
5. Select the Fluorescence Optical Kit.
6. Touch the "Door" button to automatically open the instrument door.
7. Place the sample plate on the Microplate Sample Tray. The A1 well must be
at the top right corner.
8. Touch the "Door" button again to automatically retract the sample plate
back into the instrument.
9. Touch the "Start" button.
Figure 37. Defining a fluorescence plate read.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Part# TM311
Page 44
Printed in USA.
Revised 5/10
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