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20.E. Cleaning the Injectors
We recommend thoroughly cleaning the injector(s) every 30 days.
1. Use a prepared solution of 70% ethanol to flush the injector(s) three times.
2. Place the container of 70% ethanol on the bottle holder. The stainless steel
inlet tube should be inserted so that it reaches the bottom of the bottle.
3. Place an empty Waste Collection Tray on the Microplate Sample Tray.
4. From the Instrument Control screen, go to the Read screen. Touch the
"Setup" button to run the Prime protocol.
5. Allow the solution to sit in the fluid path for 30 minutes before flushing the
injector(s).
6. Run the Custom Flush protocol nine times with 70% ethanol.
7. Empty and clean the Waste Collection Tray, and place it on the Microplate
Sample Tray.
8. Place a container of deionized water on the bottle holder with the stainless
steel inlet tube inserted to reach the bottom of the bottle.
9. From the Instrument Control screen, go to the Read screen. Touch the
"Setup" button, and run the Flush protocol.
10. Select the option for Custom Flush.
11. Select nine flush cycles.
12. When all flush cycles are complete, empty and clean the Waste Collection
Tray, and place it on the Microplate Sample Tray.
13. Flush the injector(s) three times with air. A small volume of water will
remain in the injector after the air purge.
20.F. Cleaning the Waste Collection Tray
The Waste Collection Tray prevents liquids from splashing or spilling inside
the instrument during the Prime and Flush protocols. The maximum volume
capacity of the Waste Collection Tray is approximately 50ml. Use care when
removing the Waste Collection Tray from the Microplate Sample Tray so that
no liquid spills inside the instrument.
After each prime and flush sequence, discard any fluid from the Waste
Collection Tray, and rinse it with deionized water.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Part# TM311
Page 78
Printed in USA.
Revised 5/10
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