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13. Turn on the instrument. This
will automatically power the
Injector System. Go to the
Instrument Control screen,
touch the "Read" button.
Installation is successful if an
Injector icon appears on the
Instrument Status bar (circled
on bottom of image, right). If
no Injector icon appears, verify
that the DB-15 cable is securely
connected to the correct port on
the back of the instrument.
Contact Promega Technical
Services if you need further
assistance.
18.C. Injector System Operation
Note: The injector system is capable of injecting reagent into all well formats
except 384-well plates.
The user can define the injection volume, delay time, and integration time for
each of the two injectors using the New Protocol wizard from the Home
window. This information is saved with each protocol. Injectors can be used
only with the luminescence protocols.
The left "Injector" button activates Injector 1, and the right "Injector" button
activates Injector 2.
Figure 58. "Injector" buttons.
If a protocol requires injectors, the injectors must first be primed with 900µl of
the reagent to be injected. Priming wets the fluid path and removes air bubbles
to ensure delivery of an accurate injection volume for the assay.
Unused reagent in the fluid path can be retrieved. Select the option to Reverse
Purge by touching the "Injector Setup" button on the Read screen. To prevent
clogging of the fluid lines, flush after each use.
Note: The reagent container holder can accommodate four sizes of containers:
A round flat-bottom bottle, a 50ml conical tube, a 15ml conical tube and a 15ml
test tube.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Printed in USA.
Revised 5/10
Part# TM311
Page 67
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