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10.C. PMT Activation (continued)
While the PMT is activating, you can maneuver around the software and select
other detection modalities without interfering with the PMT activation.
However, if a run is started in a different detection mode or the instrument
door is open for more than one minute, the PMT will deactivate.
Parameters and file names can be set while the PMT is activating. If the "Start"
button is touched before the PMT is completely activated, the run will begin
immediately after the 5-minute warm-up of the PMT.
10.D. Starting a Run
Easy-to-use protocol wizards can be accessed from the Home screen to set up
the run parameters. See Section 6 for protocol management. Otherwise, follow
the steps below to set up a Luminescence run.
1. From the Home screen, touch the "Instrument Control" button, then the
"Read" button (Figure 28).
2. Select and drag the "Luminescence" icon from the Protocol options to the
Protocol Composer.
3. Touch the "Plate Format" button and select the plate format that is
required.
4. Touch the "Plate" button and select the wells to be read. Green indicates a
well is selected and gray indicates a well is deselected.
Figure 28. Defining a Luminescent Plate read.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Part# TM311
Page 32
Printed in USA.
Revised 5/10
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