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10. Luminometer Operation
10.A. General Information
The Luminometer detection wavelength range is 350 to 650nm.
Microplate Recommendations
• White plates are recommended for general luminescence detection.
• Black plates are recommended primarily for bright assays, such as
ELISA, but generally will yield decreased sensitivity despite lower
background and crosstalk than white plates.
• Clear plates are not recommended due to high crosstalk.
Luciferase assay samples and reagents must be equilibrated to room
temperature prior to taking measurements to ensure optimal light intensity.
For best results, use freshly prepared reagents and follow assay protocols
carefully.
To reduce background noise, keep the interior of the instrument clean. To
reduce crosstalk from adjacent wells, always use the Microplate Sample Tray
Cover. Flush the injectors after each use and clean up any spills. See Section 20
for detailed cleaning instructions.
10.B. Microplate Sample Tray Cover
The Microplate Sample Tray Cover is installed on the Microplate Sample Tray
as part of the Luminescence module. Its primary purpose is to reduce crosstalk
signal from adjacent wells.
• The Microplate Sample Plate Cover is for use only with 96-well plates. The
cover must be removed prior to running any other plate format. See
Section 20.D for Microplate Sample Plate Cover removal.
• If any moisture appears on the Microplate Sample Tray Cover, clean the
optical lens, the mask and the interior of the instrument. See Section 20.B
for general care and cleaning instructions.
10.C. PMT Activation
The Luminescence module uses Photomultiplier Tube (PMT) detection. It
requires a 5-minute warm-up to ensure consistent results. Activating the PMT
prior to preparing the sample plate for reading will reduce the wait time
before the run.
When the Luminescence mode is selected, an "Activate PMT" button is visible
in place of the "Start" button. This reminds you to warm up the PMT before
initiating a run. After PMT activation begins, the "Start" button appears.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Printed in USA.
Revised 5/10
Part# TM311
Page 31
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