D. Ratiometric Assay - Promega E8051 Technical Manual

11.D. Ratiometric Assay

For ratiometric assays, two wavelengths can be selected for each well reading.
The results screen can display only the OD values for each wavelength (two
values for each well) or a ratio of the two readings (when the "Ratio" button is
selected). For more details on absorbance results, see Section 15.
12. UV-Vis Absorbance
12.A. General Information
The GloMax ® -Multi+ Detection System with UV-Vis Module detection has a
wavelength range of 200–800nm. The system includes four fixed filters and
two customizable filter holders for the 260nm (Paddle A) and 280nm
(Paddle B) filter paddles that are included in the six-position filters wheel. Two
absorbance wavelengths can be selected per well when running ratiometric
assays.
Microplate Recommendations
• For Visible Region measurements, black-walled and clear flat-bottom
microplates are recommended. Clear microplates can also be used.
• For UV-Vis region measurements, UV transparent plates
(Costar ® Cat.# 3635) are recommended.
Note: When using clear microplates, we recommend using the Microplate
Sample Tray Cover to reduce light scatter from adjacent wells. See Section
20.D for removing and attaching the Microplate Sample Tray Cover.
Warning: The Xenon Flash lamp emits ultraviolet light at levels that can injure
!
the eye. Do not open the instrument door while taking measurements.
12.B. Optical Filters Wheel
The Optical Filters Wheel located on the bottom of the Absorbance Detection
Head (Figure 32) holds four fixed optical filters and two customizable filter
paddles. The wavelength is easily selected by using the touch screen interface;
the appropriate filter is automatically selected by the instrument. A manual
change of the filter is not required.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Printed in USA.
Revised 5/10
Part# TM311
Page 41