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3. Follow the Setup wizard for step-by-step instructions to run a Flush
protocol.
There are two protocols used for flushing. The recommended flush protocol
uses a sequence of 3ml of deionized water, 3ml of 70% ethanol, 3ml of
deionized water and then air to flush the fluid. The second protocol allows the
user to define the number of 3ml flush cycles. This protocol does not offer an
air-only flush cycle.
Note: Remove Waste Collection Tray when priming and flushing are
complete.
18.D. Using the Dispense Option
The GloMax
®
-Multi+ Detection System Dispense option allows for the use of
the injector system to dispense reagent independent from a plate read. This
option can be used in conjunction with the Incubation option to inject reagent
and then incubate the plate prior to a plate read.
•
The injector system delivers 25–200µl in 5µl-increments of reagent to the
plate well.
•
The Dispense option can deliver reagent to all plate formats except
384-well plates.
•
It allows for the selection of either Injector 1 or Injector 2.
Figure 60. The "Dispense" Icon.
Instructions for Setting up Protocol with Dispense Option
1. Select and drag "Dispense" icon from the Protocol Options to the Protocol
Composer.
2. Set up the injector system and select Injector. Refer to Section 18.C for
instructions.
3. Enter the volume of reagent to be dispensed by pressing the white Volume
box.
4. Select plate format and wells that will be dispensed to. Refer to Section
18.C for instructions.
5. Select and drag "Incubation" icon to Protocol Composer. Refer to Section
8.B for instructions.
6. If a plate read is to follow Incubation step, select and define parameters.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Printed in USA.
Revised 5/10
Part# TM311
Page 69
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