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4.E. Terminology Used in Parameter Settings
Delay: The number of seconds between injecting the reagent and taking a
measurement. Setting a delay after the injection will allow flash-type
luminescence to fully actualize before a reading. The minimum delay value is
0.5 seconds in 0.1-second increments.
Dispense: The injector system delivers 25–200µl of reagent in 5µl increments.
This option can be used in conjunction with the Incubation icon to allow
sufficient incubation time in the plate.
Flush: The protocol used to clean the injector system after a run. The
recommended flush protocol consists of steps for washing with deionized
water, 70% ethanol, deionized water and then air-drying.
Integration: The duration of measurement per well ranging from 0.1 to 10
seconds in 0.1-second increments. In assays where noise is much lower than
signal, a 1-second integration time will yield the same sensitivity as a 10-
second integration time. Unless specified by the assay protocol, a 1-second
integration time should be sufficient.
Optical Kit: Selects a Fluorescence Optical Kit from a list.
Period: The time interval between readings of the same well ranging from 1 to
120 minutes in 1-minute increments.
Preset Protocols: A set of popular assay protocols preloaded into the
instrument for user convenience. These protocols cannot be renamed or
deleted. A user-modified version can be saved by touching the "User" button
on the "Protocol" screen.
Prime: The injector and tubing are rinsed and filled with the reagent.
Protocol Composer: The section of the Graphical User Interface (GUI) to which
the Protocol Options are dragged. This defines the order in which the protocol
will run.
Protocol Options: The list of available instrument operations that can be
selected and dragged into the Protocol Composer.
Reading: The number of times a sample plate is read. The number of reads can
be set between 1 and 99.
Reverse Purge: The protocol retrieves any unused reagent from the injector
system. After a run, this protocol can be used to push the reagent back into the
reagent bottle to reduce waste.
User Protocols: Protocols created by the user, which can be retrieved later. All
parameters including the plate well mapping are saved. These protocols can be
modified, renamed or deleted.
Volume: The volume injected into each well ranges from 25–200µl in 5µl
increments for 6- to 96-well plates. Determine the sample volume per well
before selecting an injection volume. Although the maximum volume of a
typical 96-well plate is 300µl, the recommended maximum volume per well for
use with this instrument is 250µl. Overfilling a well will result in spills and
possible damage to the instrument.
Wavelength: Selects one or two Absorbance filters from a list.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Part# TM311
Page 12
Printed in USA.
Revised 5/10
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