D. Fluorescence Data - Promega E8051 Technical Manual

15.C. Absorbance Data (continued)
Wells marked with "Ref" were designated as Reference wells. The reference
value of each wavelength reading is subtracted from each wavelength reading.
If more than one Reference well is selected, an average of the Reference values
at each wavelength is used.
Ratiometric Data
A.
Figure 43. Absorbance ratiometric results as displayed on the Read screen.
When two wavelengths are selected for a protocol, a reading at each
wavelength is taken for each sample. The results are displayed in OD units in
three possible formats.
• In partial view, reading 1 and reading 2 are displayed side-by-side in the
corresponding well (Figure 43, Panel A).
• In full view, reading 1 is displayed above reading 2 in the corresponding
well (Figure 43, Panel B).
• When the "Ratio" button is touched, the results are displayed as a single
value (Figure 43, Panel C).
The ratio value calculated for absorbance data is different than for
luminescence data. The absorbance value is calculated as:
Absorbance Value = 2

15.D. Fluorescence Data

The Relative Fluorescence Units (RFU) are displayed with two significant
digits.
Note: A value of 1 × 10
15.E. Multiplexing
The GloMax
assays. When multiplexing, read the first assay based on the protocol needed
for the reagent. To multiplex, press the "Multiplex" button displayed in the
Results screen, and select or generate the next detection protocol needed. The
resulting data will be saved together in a single file.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA
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Part# TM311
Page 50
B.
nd reading – 1
33 indicates saturation of fluorescence signal.
® -Multi+ Detection System simplifies the process of multiplexing
C.
st reading
Printed in USA.
Revised 5/10