C. Setting Incubation Parameters Without Plate Read - Promega E8051 Technical Manual

8.C. Setting Incubation Parameters without Plate Read

Figure 17. Setting the Incubation Parameters without Plate Read.
1. Activate the Heater option (Section 8.A).
2. Select and drag the "Incubation" icon from the Protocol Options to the
Protocol Composer (Figure 17).
3. Touch the "Time" button to select the length of incubation.
4. Touch the "Temperature" button to set the desired assay temperature in
degrees Celsius (°C).
5. Touch the "Temperature Control" button to activate the Heater controls.
6. Touch the "Door" button to automatically open the instrument door.
7. Place the sample plate on the Microplate Sample Tray. Well A1 must be at
the top right corner.
8. Touch the "Door" button again to retract the sample plate back into the
instrument.
9. Press the "Start" button.
Note: For assays that require an incubation step without heat, omit
activating the Heater option on the Tools screen and do not select the
Temperature Control button.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA
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Printed in USA.
Revised 5/10
Part# TM311
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