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7. Select Next Sample to move to the next position. The
appropriate Position number will be displayed, and the
kit box information will be carried over to the next
position screen. You will not need to add the kit box
data each time. If more than one kit lot is used, the new
data can be added once the cartridge and elution tube
from the new kit have been added to the rack.
8. Repeat Steps 2 to 5 for the next sample and associated
cartridge and elution tube and place in the required
position. Select Next Sample. Repeat until all samples
are loaded.
9. If there are less than 16 samples, select Final Sample
when all of the samples are loaded. This will open the
Verify Scanned Data screen. This screen will allow you
to review the data. If any incorrect data are identified
for a sample, you can use the navigation buttons to
return to the Barcode Input screen where Position 01
will be shown. Use Next Sample to navigate to the
required sample, and add all of the data for the sample.
10. For SEV runs, once all of the data have been verified,
press the Run/Stop button.
11. For LEV runs, once all of the data have been verified,
place the cartridge rack into the instrument, and press
the Run/Stop button.
C. Performing a Sample Purification Run
Follow the on-screen instructions for SEV or LEV setup.
Figure 26. SEV Set Up screen.
Promega Corporation
2800 Woods Hollow Road • Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526 • Phone 608-274-4330
Maxwell
®
16 MDx Instrument Technical Manual
Figure 27. LEV Set Up screen.
Once all of the samples, plungers and elution tubes have
been loaded, press the Run/Stop button and close the door.
The run will begin automatically.
!
During the run, the Processing screen will display the
current run step and the run time remaining. The
instrument will automatically perform periodic
self-diagnostic tests to verify that the platform, plunger bar
and magnetic rod assembly are within calibration during
the purification procedure. If the instrument detects that
any of these parts are not within calibration during a run,
the method will pause and display Calibration Error.
Figure 28. Processing screen.
If a calibration error occurs, note the Error Code Number
displayed on the touch screen and refer to the
Troubleshooting section (Section 9) for more information.
If you are in the middle of a run, you can choose to continue
the purification procedure or to abandon the run.
Continuing the run will not damage the Maxwell
Instrument but may result in suboptimal recovery of nucleic
Important. The plungers must be placed in the correct
starting position. If the instrument goes through a run
without plungers, the magnetic rod assembly must be
removed and cleaned (see Section 8.B). The cartridges
containing the samples must be discarded.
®
16 MDx
part# TM320
15
rev. 9/09
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