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11.C. Starting a Run
Easy-to-use protocol wizards can be accessed from the Home screen to set up
the run parameters. Follow the steps below to set up an Absorbance run.
Figure 34. Setting up a single Absorbance Read.
1. From the Home screen, touch the "Instrument Control" button, and then
the "Read" button.
2. Select and drag the "Absorbance" icon from the Protocol Options to the
Protocol Composer.
3. Touch the white text button to select a protocol or modify the displayed
parameters.
4. Single- or dual-wavelengths can be selected by toggling the "Wavelength"
button (Figures 34 and 35).
5. Touch the "Wavelength Filter Selection" button to select a wavelength.
There is an option for each of the installed filters. For custom filters, select
either position A or B.
6. Touch the "Plate" button and select wells to be read. Green indicates a well
is selected, gray indicates a well is deselected and Ref is for reference.
7. Touch the "Door" button to automatically open the instrument door.
8. Place the sample plate on the Microplate Sample Tray. Well A1 must be at
the top right corner.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Printed in USA.
Revised 5/10
Part# TM311
Page 39
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