Hitachi TM3030 Plus Instruction Manual page 138

1. 5 kV
This is an observation condition well-suited for observing fine features on the specimen
surface. Damage to biological samples during the observation can be minimized. In the
case of the charge-up reduction mode, images can become rough. [5 kV] can be selected
together with [Low mag.], [Middle mag.], or [High mag.]. The current magnification
condition is indicated below the [5 kV] button. If it is during observation to select [5 kV],
[Auto B/C] adjustment is executed. Select a desired magnification condition, according to
the specimen and magnification.
1a. Low mag.
This observation condition tends to produce contrasty smooth images, suitable for
observation at a magnification of 15x to 2,000x. This magnification is only reference
value. Set the advanced value depending on samples and purpose.
When [Low mag.] is selected, the [l] mark is displayed in the information area located
in the lower right part of the image observation area. (see 4.3.2)
Figure 4.2.4-2 Observation Condition Settings (5 kV - Low Mag.)
1b. Middle mag.
An electron beam is focused more than [Low mag.], achieving relatively high
resolution. However, in some cases, contrast or image degradation can occur
depending on the observed sample or magnification. This condition is suitable for
observation at a magnification of 2,000x to 6,000x. This magnification is only
reference value. Set the advanced value depending on samples and purpose.
When [Middle mag.] is selected, the [m] mark is displayed in the information area
located in the lower right part of the image observation area. (see 4.3.2)
Figure 4.2.4-3 Observation Condition Settings (5 kV - Middle Mag.)
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