Beckman Coulter UniCel DxC 600 Instructions For Use Manual page 84

System Description
Cartridge Chemistry: Calibration Theory
Table 1.17 Calculation of Calibration Factors for Endpoint and Rate Chemistries (Continued)
Type
Blanked Rate
Chemistries
1-48
FOR HIGH CALIBRATOR LEVEL:
(Reaction Rate - Blank Rate) = Delta Rate
(Reaction Rate - Blank Rate) = Delta Rate
(Delta Rate + Delta Rate
rep1
FOR LOW CALIBRATOR LEVEL:
(Reaction Rate - Blank Rate) = Delta Rate
(Reaction Rate - Blank Rate) = Delta Rate
(Delta Rate + Delta Rate
rep1
Offset = Cal Set Point (hi) - [Cal Factor × Delta Rate
Sample values are calculated by the following equation:
[(Reaction Rate - Blank Rate) × Cal Factor] + offset = sample value
IMPORTANT
For single point linear calibration, the low calibrator is a fixed zero point
and the offset is equal to zero.
Formula
rep1
rep2
) × 0.5 = Delta Rate (hi)
rep2 avg
rep1
rep2
) × 0.5 = Delta Rate (lo)
rep2 avg
(hi)]
avg
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