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Beckman Coulter P/ACE MDQ Instructions For Use Manual

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A36419AC
March 2012
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821
Instructions For Use
P/ACE MDQ
Installation & Maintenance
32 Karat 8.0

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Summary of Contents for Beckman Coulter P/ACE MDQ

  • Page 1 Instructions For Use P/ACE MDQ Installation & Maintenance 32 Karat 8.0 A36419AC March 2012 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821...
  • Page 2 Made in the USA Trademarks BECKMAN COULTER, the stylized logo, P/ACE, 32 Karat, and MDQ are trademarks of Beckman Coulter, Inc., and are registered with the USPTO. All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.

  • Page 3: Table Of Contents

    Installing the Filter Housing into the Detector ....... 24 Installing the LIF Detector into the P/ACE MDQ ......25 Preparing the Cartridge for LIF Usage .
  • Page 4 UV Detector Outputs ..........28 LIF Detector Outputs .
  • Page 5 P/ACE MDQ™ MDQ Buffer Reservoir Tray ........
  • Page 6 32 Karat 8.0 PN A36419AC...
  • Page 7 Company, which are inconsistent or in conflict with the terms of this warranty, shall not be binding upon the Company unless reduced in writing and approved by an expressly authorized officer of the Company. P/ACE MDQ Installation & Maintenance Manual PN A36419AC...
  • Page 8 Warranty 32 Karat 8.0 viii PN A36419AC...
  • Page 9 This section provides safety information and instructions for the hardware and accessories of the system. It is broken down into the following subsections: Symbols Waste Management and Recycling Chemical and Biological Safety Electrical Safety Moving Parts Safety P/ACE MDQ Installation & Maintenance Manual PN A36419AC...
  • Page 10 Safety Information Safety Symbols Safety Symbols The following information describes the notes and warnings used in this document. Beckman Coulter recommends that you review this information before using the system. WARNING Paragraphs marked by “WARNING” alert you of a potential hazard to your personal safety if you do not adhere to the information stated within the paragraph.
  • Page 11 Customers must understand and follow all laws regarding the proper decontamination and safe disposal of electrical equipment. Please contact your dealer or local Beckman Coulter office for details on the take-back program that will facilitate the proper collection, treatment, recovery, recycling, and safe disposal of Beckman Coulter products bearing this label.
  • Page 12 For this reason, any service requiring removal of a panel or otherwise overriding or disabling safety interlocks must be done by Beckman Coulter personnel only. Moving Parts Moving parts are limited to the sample handling system. Plate movement is safety interlocked through the Sample Access Cover.

  • Page 13: Section 1 Introduction

    Section 1 - Introduction Section 2 - Specifications & Site Requirements Section 3 - Hardware and Software Installation Section 4 - Maintenance Section 5 - Additional Features P/ACE MDQ Installation & Maintenance Manual PN A36419AC...
  • Page 14 Introduction General Information 32 Karat 8.0 PN A36419AC...

  • Page 15: Section 2 Specifications And Site Requirements

    Guide. This guide is sent to the installation site prior to the actual system installation. The guide provides information necessary to ensure a successful installation, e.g., environment, bench space, power requirements, etc., as well as specifications for the instrument and associated components. P/ACE MDQ Installation & Maintenance Manual PN A36419AC...
  • Page 16 Specifications and Site Requirements Introduction 32 Karat 8.0 PN A36419AC...

  • Page 17: Section 3 Hardware And Software Installation

    This section includes procedures for hardware and software installation in case it becomes necessary to relocate or reconfigure the instrument. NOTE Initial installation by anyone other than an authorized Beckman Coulter representative invalidates any warranty covering this instrument. This section is organized into four topics. The table below lists these topics in the order that they are presented.

  • Page 18: Installation - General

    Additional features provide details on installing and configuring such items as the Buffer Tray Reservoir, the External Detector Adapter, and the SS420X device. The Beckman Coulter Service Engineer will initially perform all the general installation and configuring procedures. However, these procedures will be outlined in the following paragraphs for reference purposes and for those installations where the customer provides the computer.
  • Page 19 2. Outlet Carriage Assembly CAUTION The P/ACE MDQ is heavy (155 lbs/70.5 kg). To avoid personal injury and/or possible damage to the equipment if dropped, use at least two people to lift the instrument. Lift the cartridge cover door at the front of the P/ACE MDQ.

  • Page 20: Repacking

    Cover any additional hardware and place in shipping container. Storage The P/ACE MDQ can be stored indefinitely at temperatures from 4°C to 40°C. It is not advisable to store the instrument for long periods of time below 4°C or above 40°C. If the instrument is colder or warmer, allow the instrument to warm or cool to within the operating temperature range before turning the power on and beginning the warm-up period.

  • Page 21: Installation - Hardware / Software

    Installation - Hardware / Software The following outlines the hardware/software installation process. Initially, installation will be done by the Beckman Coulter Service Engineer. The following information is for reference purposes only. The installation process includes: Installing the GPIB board in the computer Configuring the computer.

  • Page 22: Installing The Gpib Communication Interface Board

    Hardware and Software Installation Configuring the Computer Installing the GPIB Communication Interface Board The GPIB board provides the communications interface between the computer and the P/ACE MDQ instrument. Install GPIB board as follows: Remove the computer’s cover, then install the GPIB board in any available PCI slot. Refer to the manual supplied with the computer on how to remove the cover and install additional boards.

  • Page 23: Installing The Gpib Communication Interface Board Driver

    Check Enable Auto Polling I/O Time-out to 10 seconds Parallel Poll Duration to default Check EOI at end of write EOI at end of write EOS byte to 0 Click OK Step 4 Select DEV1 P/ACE MDQ Installation & Maintenance Manual PN A36419AC...
  • Page 24 Step 15 Driver should load successfully. Repeat all steps if driver loading is not successful. If after several attempts you are not successful in loading the driver, contact a Beckman Coulter Service Representative. 13. After completing installation of the GPIB board’s driver, proceed to "Installing the 32 Karat Software"...

  • Page 25: Installing The 32 Karat Software

    Installing the 32 Karat Software Installing the 32 Karat Software The following information is for reference purposes only. Beckman Coulter installs the 32 Karat Software on the systems shipped with a computer. However, a customer provided computer requires installation of the software. Refer to the following steps to install the 32 Karat Software: Start the computer.
  • Page 26 Hardware and Software Installation Installing the 32 Karat Software Figure 3.4 Welcome Screen Click Next to continue the installation. Follow the prompts. When the software installation is complete, the Setup Complete screen will be displayed. Figure 3.5 Setup Complete Screen Finish Choose , then click the...

  • Page 27: Software Licenses

    Exit the 32 Karat software. Change the computer name as desired. Delete the file C:\32Karat\_CONFIG\drvstart32c.sys. Reboot the computer. Run the 32 Karat software. This will reinstall the license with the new computer name. P/ACE MDQ Installation & Maintenance Manual PN A36419AC...

  • Page 28: Installing The P/Ace Mdq Instrument

    Figure 3.2, installed the GPIB board and its driver in the computer and installed 32 Karat Software, complete installation of the P/ACE MDQ Instrument and detector. The installation procedures covered are listed as follows. Start with the type of detector to be installed (a or b below), then proceed to remaining items (c through e).
  • Page 29 Hardware and Software Installation Installing the P/ACE MDQ Instrument Figure 3.6 UV Detector Installation 1.Cartridge Cover Door 3. Fiber Optic Cable 2. Detector Mounting Location P/ACE MDQ Installation & Maintenance Manual PN A36419AC...
  • Page 30 Hardware and Software Installation Installing the P/ACE MDQ Instrument Figure 3.7 PDA Detector Installation 1. Cartridge Cover Door 3. Fiber Optic Cable 2. Detector Mounting Location 32 Karat 8.0 PN A36419AC...

  • Page 31: Installing The Capillary Cartridge For Uv/Pda Detectors

    Capillary Cartridge Rebuild procedures located in "Maintenance" on page 33. Before you begin, you will need the capillary cartridge assembled with a capillary. Lift the cartridge cover door at the front of the P/ACE MDQ. Refer to Figure 3.8. Loosen the captive screws and lift the insertion bar.

  • Page 32: 3.10 Installing The Lif Detector

    CAUTION Some laser modules produce significant quantities of heated air. To avoid temperature-related problems with the instrument, position these lasers so that this heated air is not drawn into the P/ACE MDQ instrument. Installing the Interconnect Module If installed, remove the UV or PDA detector from the upper-right corner of the P/ACE MDQ instrument.

  • Page 33: Connecting The Laser Cable(S) To The Interconnect Module

    2. Probe Holder 5. Interlock Cover 3. Fiber Optic Connector 6. Probe Holder Installing the LIF Detector Module NOTE Prior to installing the LIF Detector Module, the filter(s) and filter housing must be installed. P/ACE MDQ Installation & Maintenance Manual PN A36419AC...

  • Page 34: Installing The Lif Detector Module

    The rejection filter prevents scattered laser light from passing into the detector. The emission filter is a band-pass filter that allows only the fluorescent signal at a selected wavelength to pass into the detector. Beckman Coulter supplies the following filters: 520 nm, 560 nm, and 675 nm emission (band-pass) 488 nm notch Depending on the application and laser, you may use any standard 0.5"...
  • Page 35 Hardware and Software Installation Installing the LIF Detector Figure 3.10 Installing Filters in the Filter Housing 1. Notch Filter 2. Emissions (Band-Pass) Filter P/ACE MDQ Installation & Maintenance Manual PN A36419AC...

  • Page 36: Installing The Filter Housing Into The Detector

    Hardware and Software Installation Installing the LIF Detector Installing the Filter Housing into the Detector Insert the filter housing into the opening in the right side of the detector. Refer to Figure 3.11. Make sure the “keyed” corner of the filter housing lines up with the “keyed” corner of the Detector Module.

  • Page 37: Installing The Lif Detector Into The P/Ace Mdq

    To avoid a possible shock hazard, power down the P/ACE MDQ instrument before proceeding with component installation. Place the LIF Detector into the P/ACE MDQ, as shown in Figure 3.11, keeping the two female connectors on the LIF Detector level with the two male connectors located on the instrument.

  • Page 38: Connecting The Probe To The Clamping Bar

    Hardware and Software Installation Installing the LIF Detector Connecting the Probe to the Clamping Bar The Cartridge must be installed in the instrument before probe can be connected. To connect the probe to the LIF Cartridge, align the two pins on the left side of the probe to the corresponding openings on the clamping bar.

  • Page 39: 3.11 Connecting The Computer To The P/Ace Mdq Instrument

    Communications Cable Power Cable Verify that the P/ACE MDQ power cable is not plugged into the AC power outlet and that the computer power is turned off. Connect the communications cable between the P/ACE MDQ and computer as shown in Figure 3.14.

  • Page 40: Analog Outputs

    Connecting the Computer to the P/ACE MDQ Instrument Analog Outputs Three analog output connectors (BNC type) are available on the P/ACE MDQ connector panel as shown in Figure 3.15. The outputs of these connectors are determined by the type of detector installed (PDA, LIF, or UV).

  • Page 41: Lif Detector Outputs

    The components included with the system must be configured before you can start using the systems. Successfully configuring the hardware indicates a successful hardware/software installation. Please refer to the P/ACE MDQ User’s Guide for details on configuring the system. 3.13 Installing the Buffer/Sample Tray During configuration of the system, the type of tray must is entered into the software.
  • Page 42 Hardware and Software Installation Installing the Buffer/Sample Tray Figure 3.16 Buffer/Sample Tray Installation 1. Inlet Sample Tray (48) or 96-Well Plate 3. Outlet Buffer Trays (36 Vials) 2. Inlet Buffer Trays (36 Vials) 4. Outlet Sample Tray (48) or 96-Well Plate 32 Karat 8.0 PN A36419AC...

  • Page 43: 3.14 Installation - Additional Features

    Installation of the Buffer Tray Reservoir is covered in "Additional Features" on page 79. External Detector Adapter The External Detector Adapter (EDA) is designed to allow the capillary to exit the P/ACE MDQ instrument for connection to external detectors or other devices such as mass spectrometers. The EDA consists of instrument modifications, software changes and hardware.
  • Page 44 Hardware and Software Installation Installation - Additional Features 32 Karat 8.0 PN A36419AC...

  • Page 45: Section 4 Maintenance

    WARNING Maintenance or repair procedures not specifically described in this manual present a risk of electrical shock or injury. Refer additional servicing to Beckman Coulter. WARNING Do not attempt to defeat any of the instrument interlocks or safety mechanisms.

  • Page 46: Rebuilding The Capillary Cartridge

    Rebuilding the Capillary Cartridge This procedure provides instructions for rebuilding and/or replacing parts, as necessary, for the P/ACE MDQ cartridge assembly. Before rebuilding the cartridge, refer to Figure 4.1, the table of rebuild components, and the following list for items required.

  • Page 47: Removing The Capillary

    Be careful when handling capillaries. If broken, the capillary ends can be very sharp and can cause a puncture wound. Figure 4.2 Removing Seal-Retainer Clips from Capillary Cartridge 1. Gently pry the seal-retainer clips away 2. Seal-Retainer Clip and Double Seal from the cartridge. P/ACE MDQ Installation & Maintenance Manual PN A36419AC...
  • Page 48 Maintenance Rebuilding the Capillary Cartridge Remove the aperture by pushing from the front of the cartridge and pulling out from the back. If the O-Ring inside the aperture does not come out, use a pair of forceps to remove it. Refer to Figure 4.3.
  • Page 49 Maintenance Rebuilding the Capillary Cartridge Grasp the capillary at the cartridge inlet and pull firmly to remove it, as shown in Figure 4.4. Figure 4.4 Removing the Capillary 1. Inlet P/ACE MDQ Installation & Maintenance Manual PN A36419AC...
  • Page 50 Maintenance Rebuilding the Capillary Cartridge Referring to Figure 4.5, remove the aperture seal clips. If seals appear to be worn, damaged, or leaking, replace the seals. Reinstall the aperture seal clips. Figure 4.5 Removing Aperture-Seal Clips 1. Gently pry the aperture clips away from 2.

  • Page 51: Replacing The Coolant Tubing And Seal

    Select the desired capillary length from the following table, then select corresponding coolant tubing from the Cartridge Tubing Kit or cut coolant tubing to desired length as specified in the table following. Use a razor blade or X-ACTO® knife to cut tubing. P/ACE MDQ Installation & Maintenance Manual PN A36419AC...
  • Page 52 Maintenance Rebuilding the Capillary Cartridge Capillary Length to Detector Total Capillary Length Coolant Tubing Length 21 cm 31.2 cm 14.0 cm* 30 cm** 40.2 cm 22.8 cm* 40 cm** 50.2 cm 32.8 cm* 50 cm 60.2 cm 42.8 cm* 60 cm 70 cm 52 cm 70 cm...
  • Page 53 11. Insert the other end of the coolant tubing in the cartridge outlet and press firmly into the cartridge base. 12. Continue to press the tubing in. 13. Push the tubing nut, ferrule, and O-Ring in and tighten the tubing nut finger tight. P/ACE MDQ Installation & Maintenance Manual PN A36419AC...

  • Page 54: Marking The Capillary

    Maintenance Rebuilding the Capillary Cartridge Marking the Capillary Referring to Figure 4.9, measure 11 cm from capillary window to the outlet end. Mark the capillary window position with a felt tip marker. NOTE Do not touch the capillary window as it is fragile and finger oils can reduce detection sensitivity. Figure 4.9 Marking Capillary Window Position 1.
  • Page 55 Figure 4.10 Inserting Capillary End into the Outlet Side of Cartridge 1. Cartridge Outlet 2. Capillary Window Carefully push and twist the capillary through the cartridge until it appears at the cartridge inlet. P/ACE MDQ Installation & Maintenance Manual PN A36419AC...
  • Page 56 Maintenance Rebuilding the Capillary Cartridge Continue by pulling the capillary through the cartridge until the capillary window appears centered in the cartridge window as shown in Figure 4.11. Figure 4.11 Centering Capillary Window in Cartridge Window 1. Capillary Window 32 Karat 8.0 PN A36419AC...

  • Page 57: Installing The Aperture Clip

    NOTE Make sure that the previous O-Ring has been removed from the aperture clip before re-installing the clip. Figure 4.12 Aligning Groove of Aperture Clip with Capillary Window 1. Aperture Clip 3. Capillary 2. Aperture P/ACE MDQ Installation & Maintenance Manual PN A36419AC...
  • Page 58 Maintenance Rebuilding the Capillary Cartridge From the front side of the cartridge, place the retainer O-Ring in the aperture clip hole. Next, using the O-Ring insertion tool, carefully press the tool down until the O-Ring is seated in the aperture clip. Refer to Figure 4.13. Figure 4.13 Seating O-Ring in Aperture-Clip Hole (Front View) 1.
  • Page 59 Be sure both the front and back edges clip into place for each clip. Refer to Figure 4.14. Figure 4.14 Inserting the Capillary-Seal Retainer Clips 1. Seal-Retainer Clips Visually inspect that the capillary ends are straight. If not, remove and reinstall the seal-retainer clips. P/ACE MDQ Installation & Maintenance Manual PN A36419AC...

  • Page 60: Trimming The Capillary Ends

    Maintenance Rebuilding the Capillary Cartridge Trimming the Capillary Ends Position the cartridge against the capillary-length template as shown in Figure 4.15. Figure 4.15 Trimming the Capillary Ends 1. Cleaving Stone 2. Template At the cartridge inlet, carefully push the capillary into the cartridge until the capillary stops against the wall of the coolant tubing.

  • Page 61: Filling Vials And Installing Caps

    Maintenance Rebuilding the Capillary Cartridge Filling Vials and Installing Caps Three different vials and one plate are available for use with P/ACE MDQ. A separate procedure is provided below for each vial. The types are: 0.5 mL vials 2.0 mL vials...

  • Page 62: Filling The 2.0 Ml Vials

    Maintenance Rebuilding the Capillary Cartridge Filling the 2.0 mL Vials Before you begin, you will need: 2.0 mL vial Red vial caps Fill vials as shown in Figure 4.17. Put the cap on the vial as shown in Figure 4.17, then press in place. Figure 4.17 Filling 2.0 mL Vials 1.
  • Page 63 200 μL of sample solvent or water in the bottom of the vial holder and by injecting small samples as soon as possible. Figure 4.18 Filling PCR Vials 1. Gray Cap 4. PCR Vial Holder 2. PCR Vial 5. Maximum Fill Level 3. PCR Spring P/ACE MDQ Installation & Maintenance Manual PN A36419AC...

  • Page 64: Filling The 96-Well Plates

    Before you begin, you will need: 96-well plate CAUTION The P/ACE MDQ is not intended to accommodate volatile materials in 96-well plates. Volatile solvents may release hazardous or flammable vapors leading to a risk of fire of explosion. Solvent vapors may also damage the instrument.

  • Page 65: Installing The Uv Detector Wavelength Filters

    From Direct Control, place trays in LOAD position Lift the cartridge cover door at the front of the P/ACE MDQ. Turn the system power off. Loosen the two thumb screws and lift the insertion bar. Remove the capillary cartridge from the interface block.
  • Page 66 Maintenance Rebuilding the Capillary Cartridge Loosen the two thumb screws and remove the optics source assembly. Place the assembly on a clean work surface. Remove the filter wheel access cover and rotate the filter wheel until the desired filter is visible.
  • Page 67 15. Reinstall the capillary cartridge in the interface block. 16. Lower the insertion bar and tighten the two thumb screws. 17. Close the cartridge cover door. 18. Turn the system power on and start the 32 Karat Software. P/ACE MDQ Installation & Maintenance Manual PN A36419AC...
  • Page 68 Maintenance Rebuilding the Capillary Cartridge 19. From the Instrument screen File Menu , select Configuration . The 32 Karat Software Configuration dialog box appears as shown in Figure 4.23. 20. In the Filter dialog, enter the position and type for each of the installed filters. Figure 4.23 Configuration Dialog Box 32 Karat 8.0 PN A36419AC...

  • Page 69: Calibrating The Pda Detector

    Figure 4.25. Figure 4.25 PDA Detector Parameters Click Calibrate to begin the calibration sequence. Calibration requires approximately 2 minutes. Do not attempt to access the instrument during the calibration process. P/ACE MDQ Installation & Maintenance Manual PN A36419AC...

  • Page 70: Changing The Lamp

    7/64” hex head wrench WARNING If the P/ACE MDQ has been on for any length of time, the deuterium lamp will be very hot. To avoid burning your fingers, allow the lamp to cool before replacing it. Referring to Figure 4.26, proceed as follows:...
  • Page 71 Place the assembly on a clean work surface. Figure 4.27 Removing the Optics Assembly 1. Optics Source Assembly 2. Thumb Screws (2) Open the deuterium lamp access cover on the back of the optics source assembly. P/ACE MDQ Installation & Maintenance Manual PN A36419AC...
  • Page 72 Maintenance Rebuilding the Capillary Cartridge Disconnect the lamp power plug. Refer to Figure 4.28. Figure 4.28 Disconnecting the Lamp Power Plug 1. Power Plug 10. Using a 7/64” hex head wrench, remove the two hex screws securing the deuterium lamp and remove the lamp from the lamp housing.
  • Page 73 21. Select . The Lamp Hours of Use dialog box appears as shown in Figure 4.31. 22. Enter a value of zero in the dialog box. Figure 4.31 Lamp Hours of Use Dialog P/ACE MDQ Installation & Maintenance Manual PN A36419AC...

  • Page 74: Cleaning The Fiber Optics (Uv And Pda Detectors)

    From Direct Control, place the trays in the LOAD position. Lift the cartridge cover door at the front of the P/ACE MDQ. Refer to Figure 4.32. Turn the system power off. Disconnect the fiber optic cable at the detector first, then at the clamp arm.
  • Page 75 Maintenance Rebuilding the Capillary Cartridge Figure 4.32 Location of Fiber Optic Cable 1. Cartridge Cover Door 2. Fiber Optic Cable (PDA Detector, UV Detector is similar) P/ACE MDQ Installation & Maintenance Manual PN A36419AC...

  • Page 76: Lif Care And Maintenance

    LIF Care and Maintenance WARNING The Beckman Coulter 488 nm and 635 nm laser modules operate at high voltages and emit laser light that can cause serious damage to the eyes. Servicing of the laser should be done only by a Beckman Coulter Field Service Representative.
  • Page 77 Rebuilding the Capillary Cartridge Double-click on the LIF module displayed in the configured modules pane of the P/ACE System MDQ window. The P/ACE MDQ Instrument Configuration window will be displayed. Figure 4.33 P/ACE System MDQ Configuration Window Click on the LIF Calibration Wizard to begin the calibration.

  • Page 78: Manual Calibration

    Maintenance Rebuilding the Capillary Cartridge Manual Calibration Select the Manual Option button to manually input the values of the Calibration Correction Factors. This selection bypasses the calibration test mix to determine the correction factors. Figure 4.35 Calibration Wizard Calibration Correction Factor Input the Calibration Correction Factor for each of the detector channels.
  • Page 79 When the calibration is complete, the final step in the calibration wizard prompts you to accept or cancel the correction factor calculated in the calibration. Figure 4.39 Calibration Wizard Calculated Calibration Factor P/ACE MDQ Installation & Maintenance Manual PN A36419AC...

  • Page 80: Calibration Standard

    Maintenance Rebuilding the Capillary Cartridge Calibration Standard Changes can be made to the calibration procedure if a different fluorescent compound is used or if the capillary diameter is changed. After the detector has been calibrated using the recommended procedure and test mix, run the method below using the new calibration standard and capillary.

  • Page 81: Instrument Care

    This concludes the Calibration Wizard - Auto Configuration process. For additional information, refer to the Online Help. Instrument Care When P/ACE MDQ service is requested, the instrument must be decontaminated. The following procedure outlines these requirements. Before you begin, you will need:...
  • Page 82 Maintenance Instrument Care Referring to Figure 4.40, proceed as follows: Lift the cartridge cover door at the front of the P/ACE MDQ. Turn the system power off. CAUTION All surfaces that come into contact with samples must be treated. Notify the service engineer of the type of sample used and the manner of decontamination.

  • Page 83: Cleaning The Interface Block, Ejectors And Electrodes

    Remove the capillary cartridge from the interface block. Remove the ejector covers and ejectors for cleaning, as shown in Figure 4.41. Figure 4.41 Cleaning Interface Block and Ejectors 1. Ejector/Electrode Location 2. Injector Covers and Spring Retainers P/ACE MDQ Installation & Maintenance Manual PN A36419AC...

  • Page 84: Refilling The Coolant

    Referring to Figure 4.42, proceed as follows: Lower the coolant fill door at the front of the P/ACE MDQ. Attach the coolant fill adapter to the coolant fill port. Pour approximately 120 mL of coolant into the syringe.

  • Page 85: Replacing The Electrodes

    Referring to Figure 4.43, proceed as follows: Lift the cartridge cover door. Loosen the two thumb screws and lift the insertion bar. Remove the capillary cartridge from the interface block. P/ACE MDQ Installation & Maintenance Manual PN A36419AC...

  • Page 86: Cleaning

    Maintenance Replacing the Electrodes Figure 4.43 Cleaning and Replacing the Electrodes 1. Cartridge Cover Door 3. Interface Block 2. Insertion Bar 4. Ejector/Electrode Location Cleaning The electrodes are attached to the underside of the interface block as shown in Figure 4.43. To access the electrodes for cleaning, remove the ejector and ejector cover in front of the electrode as shown in Figure 4.44.

  • Page 87: Replacement

    Using a #2 flat tip screwdriver, loosen the nylon screw in the interface block two or three turns as required and pull down to remove the electrode. Replace with a new electrode and tighten the nylon screw until snug. Reinstall the ejector in front of the electrode. P/ACE MDQ Installation & Maintenance Manual PN A36419AC...

  • Page 88: Replacing The Quad-Rings

    Maintenance Replacing the Quad-Rings Reinstall the spring retainer and ejector cover. Reinstall the capillary cartridge in the interface block. Lower the insertion bar and tighten the two thumb screws. Close the cartridge cover door. Replacing the Quad-Rings If coolant is leaking between the interface block and cartridge, the quad-rings may require replacement.

  • Page 89: Replacing The Fuses

    Be sure to replace the fuses with the correct type and rating for continued protection against the risk of fire and/or improper instrument operation. If the fuses continue to blow after being replaced, contact Beckman Coulter service for additional assistance. Visually inspect or verify fuse continuity with an ohmmeter and replace the fuses with the...
  • Page 90 Maintenance Replacing the Fuses 32 Karat 8.0 PN A36419AC...

  • Page 91: Section 5 Additional Features

    Additional Features Introduction Additional Features Introduction This section includes instructions for installing and configuring the following available devices/accessories for P/ACE MDQ: SS420X (External Unit) Buffer Reservoir Tray External Detector Adapter P/ACE MDQ Installation & Maintenance Manual PN A36419AC...

  • Page 92: Connecting Detectors

    Additional Features Connecting Detectors Connecting Detectors Instrument connections (analog input, triggers, external events) are made using the Terminal Panel supplied with the system. The Terminal Panel is located inside the black cover. To access the Terminal Panel, remove the four screws holding the box cover. Figure 5.1 TP420 Terminal Panel The Terminal Panel contains the following set of connections which are used in connecting the instrument(s) to the host computer:...

  • Page 93: Connecting (Remote Start) Trigger Signals

    Outputs 1 & 2 close on computer power on and open when the 32 Karat Software is started. Outputs 3 & 4 Outputs 3 through 8 open on computer power on and remain open until closed by an external event. P/ACE MDQ Installation & Maintenance Manual PN A36419AC...

  • Page 94: Connecting External Devices

    Additional Features Connecting Detectors Connecting External Devices External devices are connected using the Digital out connectors on the Terminal Panel. The SS420X inputs can detect switch closures or can be driven by TTL compatible logic. The output switches are open collector types.These switches do not supply power to the circuit. For relay connections, it is not necessary to install a pull-up resistor between the output connector and +5 V.

  • Page 95: Ss420X External Unit

    In order to reduce noise, always use a shielded twisted pair cable for each detector connection. The two wires are used for the signal High (+) and signal Low (-) connections (e.g. CH 1+ and CH 1-). P/ACE MDQ Installation & Maintenance Manual PN A36419AC...

  • Page 96: Connecting Start Inputs (Trigger Signals)

    NOTE In order for the trigger and ready relays to operate properly, the trigger and/or ready connection channel must be specified in the system configuration screen. To access this screen, select Configuration from the P/ACE MDQ main menu, then Instruments. Hold Off Injection...

  • Page 97: Configuring The Ss420X External Device

    Enter the base address for the hardware value set on the board. The recommended value is 360 hex. Accept other defaults. Click to accept values. Figure 5.3 SS420X Configuration Screen P/ACE MDQ Installation & Maintenance Manual PN A36419AC...

  • Page 98: P/Ace™ Mdq External Detector Adapter

    The EDA-only cartridge accommodates capillaries down to an external diameter of 150 μm. With the EDA-only cartridge, the P/ACE MDQ detector is not available and the length of capillary from the point of sample injection to the outside of the instrument is substantially reduced.

  • Page 99: Installing The Capillary In The Standard Cartridge

    Grasp the capillary at the capillary adapter body and it. Pull it until the window area is centered in the aperture. Install an aperture plug and O-Ring as described in the P/ACE MDQ manual. If a new seal is not to be installed, go to step 11. After removing the nut, install a new seal (PN 144818) on the capillary emerging from the capillary body.

  • Page 100: Installing The Capillary In The Eda-Only Cartridge

    Install an aperture plug as described in the P/ACE MDQ manual. Note that the capillary does not pass through the aperture plug in this cartridge. Once installed, the plug may be left in place.

  • Page 101: Installing The Cartridge In The Instrument

    Figure 5.7 Cartridge Installation Programming for EDA Operation The EDA mode must be selected in the P/ACE MDQ 32 Karat Software in order for the adapter to function properly. The EDA option is available in the Voltage, Current, and Power dialogs in...

  • Page 102: Connecting To An External Device

    P/ACE MDQ EDA Accessory Kit Replacement skins for right and back panel, interface upright, lower side panel assembly, banana jack, banana plug, grounding harness, EDA cartridge assembly, EDA capillary adapter assembly, EDA instruction sheet and installation by Beckman Coulter Service Engineer. 144829...

  • Page 103: P/Ace Mdq™ Mdq Buffer Reservoir Tray

    P/ACE MDQ™ MDQ Buffer Reservoir Tray P/ACE MDQ™ MDQ Buffer Reservoir Tray The P/ACE MDQ Buffer Reservoir Tray (PN 144824) has two large capacity buffer reservoirs for use during capillary electrophoretic separations. Each reservoir holds approximately 25 mL of buffer. The tray can simultaneously accommodate ten standard 2 mL buffer vials. These smaller vials are useful for high pressure rinse and wash solutions.

  • Page 104: Installing The Tray

    The tray replaces the 36-position buffer tray used in the BI (buffer inlet) and BO (buffer outlet) positions on the P/ACE MDQ sample handling system. To install the new trays insert them in the white tray holders with the large reservoirs to the right (as viewed from the front of the instrument).

  • Page 105: Solvent Compatibility

    The effect of combinations of chemicals may be different from the effect of the individual chemicals alone. IMPORTANT Beckman Coulter does not warrant (express or implied) that the information in this chart is accurate or complete. Solvent...
  • Page 106 Additional Features P/ACE MDQ™ MDQ Buffer Reservoir Tray 32 Karat 8.0 PN A36419AC...
  • Page 108 © 2012 Beckman Coulter, Inc. All Rights Reserved...

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