Beckman Coulter Cytomics FC 500 Manual
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Beckman Coulter Cytomics FC 500 Manual

Special procedures with cxp software
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See also: Instructions for Use Manual , User Manual
Cytomics FC 500
With CXP Software
Special Procedures
PN 175572B (June 2004)
Beckman Coulter, Inc.
Fullerton, CA 92835

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Summary of Contents for Beckman Coulter Cytomics FC 500

  • Page 1 Cytomics FC 500 With CXP Software Special Procedures PN 175572B (June 2004) Beckman Coulter, Inc. Fullerton, CA 92835...

  • Page 2: Warnings And Precautions

    This equipment is used in a manner other than specified. Operate the instrument as instructed in the Product Manuals. r You introduce software that is not authorized by Beckman Coulter into your computer. Only operate your system’s computer with software authorized by Beckman Coulter.

  • Page 3: Revision Status

    REVISION STATUS Issue A, Initial Issue, 6/03 CXP Software Version 1.0. Initial issue for customer distribution. Issue B, 6/04 CXP Software Version 2.0. Additions, changes or deletions were made at these locations: 1-3, 1-5, 1-6, 1-10, 1-14, 1-24, 1-27, 1-31, 1-31, 1-32, 1-36, 1-41, 1-43, 1-46, 1-50, 2-8, 2-13, 2-22, 2-26, 2-30, 2-35, 2-38, 2-41, 2-47, 2-54, 2-55, 2-58, 2-59 through A-7.
  • Page 4 REVISION STATUS PN 175572B...

  • Page 5: Table Of Contents

    CONTENTS WARNINGS AND PRECAUTIONS REVISION STATUS, iii INTRODUCTION, ix USING YOUR Cytomics FC 500 MANUALS, ix ABOUT THIS MANUAL, ix CONVENTIONS, x To Choose A Command With The Keyboard, xi Dialog Box, xi Description of Reporting Units, xi GRAPHICS, xi...
  • Page 6 CONTENTS Prepare to Clean the Vacuum Trap, 1-32 Find and Pull Out the Vacuum Trap, 1-33 Rinse and Return the Vacuum Trap to Its Bracket, 1-34 1.11 CLEAN THE SAMPLING SYSTEM, 1-37 Levels of System Cleaning, 1-37 When to Clean the Sampling System, 1-37 Routine and Sample Head Cleaning Procedures, 1-37...
  • Page 7 CONTENTS Install the Optical Filter Plate, 2-46 2.13 REPLACE A FILTER IN THE OPTICAL FILTER PLATE, 2-48 2.14 POSITION THE FIELD STOP , 2-54 Procedure, 2-54 2.15 RESET THE CIRCUIT BREAKERS, 2-56 Procedure 1, 2-57 Procedure 2, 2-60 2.16 ADJUST THE HENE LASER, 2-62 MAINTENANCE AND SERVICE LOGS, A-1...
  • Page 8 CONTENTS viii PN 175572B...

  • Page 9: Introduction

    INTRODUCTION This introductory section contains the following topics: USING YOUR Cytomics FC 500 MANUALS ABOUT THIS MANUAL CONVENTIONS, and GRAPHICS. USING YOUR Cytomics FC 500 MANUALS The manuals listed below are available as PDF files in the FC 500 CXP software and the Operator’...

  • Page 10: Conventions

    INTRODUCTION CONVENTIONS CONVENTIONS This manual uses the following conventions: Throughout this manual your FC 500 is also referred to as the system or instrument. Bold font indicates a software option, such as Cytometer Italics font indicates screen text displayed on the instrument, such as Preparing Samples.

  • Page 11: To Choose A Command With The Keyboard

    INTRODUCTION GRAPHICS To Choose A Command With The Keyboard After you press Þ, each menu name has one letter underlined to indicate which letter to use to pull down the menu. For example, the letter F in the File menu is underlined, press F to pull down the File menu;...
  • Page 12 INTRODUCTION GRAPHICS PN 175572B...

  • Page 13: Cleaning Procedures

    CLEANING PROCEDURES WHAT THIS CHAPTER EXPLAINS This chapter explains how to clean the: Sample system (daily) before the daily shutdown procedure, CLEAN THE SAMPLING SYSTEM MCL sample head and sample probe (weekly), CLEAN THE MCL SAMPLE HEAD AND THE SAMPLE PROBE.

  • Page 14: Clean The Air Filters

    CLEANING PROCEDURES CLEAN THE AIR FILTERS CLEAN THE AIR FILTERS Clean the air filters weekly per Table 6.1, Cleaning Schedule in the Instructions For Use manual. It is easiest to clean the air filters after performing the shutdown procedure. Instructions and graphics are given for both Power Supply configurations. Use the procedure (Procedure 1 Procedure...

  • Page 15: Prepare To Clean The Air Filters

    CLEANING PROCEDURES CLEAN THE AIR FILTERS Prepare to Clean the Air Filters Power the Cytometer OFF Unplug both Power Supply power cords b from the wall outlet. Pull off each filter cover. Even though the covers look like they are screwed in, they are not.

  • Page 16: Rinse And Return The Air Filters

    CLEANING PROCEDURES CLEAN THE AIR FILTERS Gently pinch and pull out each filter. Handle them gently to avoid damaging them. Rinse and Return the Air Filters Rinse each filter in water b, and then wring it out c. Set each filter aside and let it dry out for about 30 minutes.
  • Page 17 CLEANING PROCEDURES CLEAN THE AIR FILTERS Return each filter into its holder. Replace any torn filters. Put each filter cover back on. Plug in both Power Supply power cords b into the wall outlet. PN 175572B...

  • Page 18: Procedure 2

    CLEANING PROCEDURES CLEAN THE AIR FILTERS Power the Cytometer Only ON Power the Cytometer and CXP Software Record that the air filters were cleaned on the electronic Maintenance Log. Perform the Daily Startup procedure before running samples. Procedure 2 If you have the Voltage-specific Power Supply configuration: Follow Procedure 2...

  • Page 19: Location Of Air Filters

    CLEANING PROCEDURES CLEAN THE AIR FILTERS Location of Air Filters The instrument has four air filters located on the: Power Supply, Back Panel (2). Cytometer Back Panel (2) POWER MODULE LOGIC ANALOG CONTROL FIBER OPTIC SCOPE A TRANS SCOPE B PRESSURE VACUUM WASTE...
  • Page 20 CLEANING PROCEDURES CLEAN THE AIR FILTERS Pull off each filter cover. Even though the covers look like they are screwed in, they are not. The filter covers are made of flexible plastic; they snap out when you pull them. Grab a segment of the grille between your thumb and index finger and then pull.

  • Page 21: Rinse And Return The Air Filters

    CLEANING PROCEDURES CLEAN THE AIR FILTERS Rinse and Return the Air Filters Rinse each filter in water b, and then wring it out c. Set each filter aside and let it dry out for about 30 minutes. Use paper towels to check that each filter is completely dry.
  • Page 22 CLEANING PROCEDURES CLEAN THE AIR FILTERS Put each filter cover back on. Plug in both Power Supply power cords b into the wall outlet. Power the Cytometer Only ON Power the Cytometer and CXP Software Record that the air filters were cleaned on the electronic Maintenance Log.

  • Page 23: Open/Remove The Instrument Covers

    CLEANING PROCEDURES OPEN/REMOVE THE INSTRUMENT COVERS Perform the Daily Startup procedure before running samples. OPEN/REMOVE THE INSTRUMENT COVERS Open the Front Cover Click S for an overview. Pull the front cover forward b and then lift straight up c. PN 175572B 1-11...
  • Page 24 CLEANING PROCEDURES OPEN/REMOVE THE INSTRUMENT COVERS Push the front cover back until you hear both sides click and lock in place. You only need to open the front cover this much if you are going to perform a procedure such as: REMOVE THE REAGENT CONTAINERS REPLACE THE REAGENT...
  • Page 25 CLEANING PROCEDURES OPEN/REMOVE THE INSTRUMENT COVERS Close the Front Cover Click S for an overview. While holding the bottom of the front cover with one hand, pull out the release pin with your free hand. Switch hands so your other hand is holding the bottom of the front cover.
  • Page 26 CLEANING PROCEDURES OPEN/REMOVE THE INSTRUMENT COVERS Using both hands, pivot the front cover forward until it is vertical. WARNING Risk of operator injury. To prevent injury, hold your hands flat on the sides of the cover. Ensure your fingers are not behind the cover as you push down and in to close it.
  • Page 27 CLEANING PROCEDURES OPEN/REMOVE THE INSTRUMENT COVERS Remove the Front Left Side Panel Click S for an overview. Power the Cytometer OFF. Unscrew the two bolts that attach the left side panel to the front frame b. Snap off the left side panel c. 1-15 PN 175572B...
  • Page 28 CLEANING PROCEDURES OPEN/REMOVE THE INSTRUMENT COVERS Replace the Front Left Side Panel Click S for an overview. Snap on the left side panel b. Screw in the two bolts to attach the left side panel to the front frame c. Power the Cytometer Only ON Power the Cytometer and CXP Software...

  • Page 29: Open The Mcl Cover

    CLEANING PROCEDURES OPEN/REMOVE THE INSTRUMENT COVERS Open the MCL Cover Lift up the MCL cover. Close the MCL Cover Close the MCL cover. PN 175572B 1-17...

  • Page 30: Put The Cytometer In The Idle Mode

    CLEANING PROCEDURES PUT THE CYTOMETER IN THE IDLE MODE PUT THE CYTOMETER IN THE IDLE MODE To clean, replace, or fill the reagent containers you need to put the Cytometer in the Idle mode. To put the Cytometer in the Idle mode: Wait about 10 seconds for the Cytometer to depressurize.
  • Page 31 CLEANING PROCEDURES REMOVE THE REAGENT CONTAINERS Open the Front Cover. Pull open the reagent drawer until it stops. b Reagent container connectors. c Cleaning agent container. d Sheath fluid container. Note: The reagent drawer sits in a self-locking track so it only opens part way and stops.
  • Page 32 CLEANING PROCEDURES REMOVE THE REAGENT CONTAINERS Disconnect the tubing on the top of each reagent container by pushing in on the metal clips on the connectors. Press down on the left locking tab and press up on the right locking tab of the reagent drawer.
  • Page 33 CLEANING PROCEDURES REMOVE THE REAGENT CONTAINERS Disconnect each sensor at the back of the drawer by sliding its sleeve out: The sensor for the sheath fluid container (shown here) is on the right. The sensor for the cleaning agent container is on the left. Unfasten the flexible strap that holds the sheath fluid filter in place.
  • Page 34 CLEANING PROCEDURES REMOVE THE REAGENT CONTAINERS Lift up the sheath filter to loosen its connected tubing. Remove the container from the drawer, unscrew the cap and empty the container as completely as possible (sheath fluid container is shown). Clean up any spills or debris in the drawer.

  • Page 35: Clean The Sheath Fluid Container

    CLEANING PROCEDURES CLEAN THE SHEATH FLUID CONTAINER CLEAN THE SHEATH FLUID CONTAINER IMPORTANT Misleading results could occur if you contaminate the sheath fluid container. Be careful not to contaminate the sheath fluid container. Do not let your fingers, paper towels, or other objects touch the inside of the container or the inside of its cap.
  • Page 36 CLEANING PROCEDURES CLEAN THE SHEATH FLUID CONTAINER Empty the container as completely as possible. Record that the sheath container was cleaned on the electronic Maintenance Log. REPLACE THE REAGENT CONTAINERS to replace the sheath fluid container. 1-24 PN 175572B...

  • Page 37: Clean The Cleaning Agent Container

    CLEANING PROCEDURES CLEAN THE CLEANING AGENT CONTAINER CLEAN THE CLEANING AGENT CONTAINER Remove and clean the cleaning agent container every 60 days. See Table 6.1, Cleaning Schedule in the Instructions For Use manual. Clean a new cleaning agent container before placing it into the reagent drawer. REMOVE THE REAGENT CONTAINERS to remove the cleaning...
  • Page 38 CLEANING PROCEDURES CLEAN THE CLEANING AGENT CONTAINER Empty the container as completely as possible. Position a funnel into the cleaning agent container. Pour about 50 to 100 mL of fresh ® COULTER CLENZ cleaning agent or equivalent into the cleaning agent container.
  • Page 39 CLEANING PROCEDURES CLEAN THE CLEANING AGENT CONTAINER Empty the container as completely as possible. Record that the cleanse container was cleaned on the electronic Maintenance Log. REPLACE THE REAGENT CONTAINERS to replace the cleaning agent container. PN 175572B 1-27...

  • Page 40: Replace The Reagent Containers

    CLEANING PROCEDURES REPLACE THE REAGENT CONTAINERS REPLACE THE REAGENT CONTAINERS Use this procedure to return a cleaned reagent container into the reagent drawer. Put the clean reagent container back into the drawer. Check that the sensor connector at the back of the container goes through the hole in the back of the reagent drawer.
  • Page 41 CLEANING PROCEDURES REPLACE THE REAGENT CONTAINERS Slide the reagent drawer back in part way. Keep the neck of the reagent container out. Reconnect the tubing assembly by pushing down on the tubing inserts so that the tubing snaps into the connector.
  • Page 42 CLEANING PROCEDURES REPLACE THE REAGENT CONTAINERS Check that: The sheath filter vent port b is above the connector c. The tubing is not kinked or twisted. Close the reagent drawer. 1-30 PN 175572B...

  • Page 43: 1.10 Clean The Vacuum Trap

    CLEANING PROCEDURES CLEAN THE VACUUM TRAP WARNING Risk of operator injury. To prevent injury, hold your hands flat on the sides of the cover. Ensure your fingers are not behind the cover as you push down and in to close it. Close the Front Cover.
  • Page 44 CLEANING PROCEDURES CLEAN THE VACUUM TRAP Prepare to Clean the Vacuum Trap Power the Cytometer OFF Unplug both Power Supply power cords b from the wall outlet. Open the Power Supply front door. FILTER FILTER WATER TRAP TRAP SYSTEM POWER WATER TRAP TRAP...
  • Page 45 CLEANING PROCEDURES CLEAN THE VACUUM TRAP Find and Pull Out the Vacuum Trap The vacuum trap is the trap on the left. Lift the vacuum trap assembly out of its bracket so that you can grasp the top of the assembly. WARNING To prevent injury, avoid skin contact with the vacuum trap and its associated tubing.
  • Page 46 CLEANING PROCEDURES CLEAN THE VACUUM TRAP Rinse and Return the Vacuum Trap to Its Bracket Rinse the vacuum trap with water, and then shake out the excess water. Insert the white center post, pointed end up, into the vacuum trap assembly. If the white center post in the vacuum trap assembly is stuck in the up position b, pull it into the down...
  • Page 47 CLEANING PROCEDURES CLEAN THE VACUUM TRAP Carefully align the threads on the vacuum trap jar with the threads on the vacuum trap assembly and screw the vacuum trap back into place. Return the vacuum trap assembly to its bracket. Wipe up any spills. FILTER WATER TRAP...
  • Page 48 CLEANING PROCEDURES CLEAN THE VACUUM TRAP Plug in both Power Supply power cords b into the wall outlet. Power the Cytometer Only ON Power the Cytometer and CXP Software Check that no error messages are displayed. Note: If an error message appears, see Table 6.2, Error Messages in the Instructions For Use manual for possible causes and operator actions.
  • Page 49 CLEANING PROCEDURES CLEAN THE SAMPLING SYSTEM 1.11 CLEAN THE SAMPLING SYSTEM Routine daily cleaning helps to minimize instrument downtime. Levels of System Cleaning The two levels of cleaning for the system are: Routine cleaning followed by sample head/probe cleaning. Vacuum line cleaning. When to Clean the Sampling System Routine and Sample Head Cleaning Procedures Perform both the routine and the sample head/probe cleaning procedures:...
  • Page 50 CLEANING PROCEDURES CLEAN THE SAMPLING SYSTEM Routine Cleaning Procedure Perform this procedure as often as described in the heading When to Clean the Sampling System. WARNING The cleaning solution is hazardous and can cause personal injury or damage clothing. Beckman Coulter urges its customers to comply with all national health and safety standards such as the use of barrier protection.
  • Page 51 CLEANING PROCEDURES CLEAN THE SAMPLING SYSTEM Load the carousel: b Put the test tube of bleach solution into carousel position 1. c Put three freshly prepared tubes, each containing about 2 mL of distilled water or IsoFlow sheath fluid, into positions 2, 3, and 4 of the carousel.
  • Page 52 CLEANING PROCEDURES CLEAN THE SAMPLING SYSTEM Put the carousel into the MCL sample loader. Close the MCL cover. Enter the Carousel No. in the Worklist. The tube numbers Location automatically appear. 1-40 PN 175572B...
  • Page 53 CLEANING PROCEDURES CLEAN THE SAMPLING SYSTEM When the cleaning panel is done, remove the carousel. Close the MCL cover. Record that the routine cleaning procedure was performed on the electronic Maintenance Log. Before running samples: to initialize the system. Testing for Residual Stain If you use vital stains such as propidium iodide, ethidium bromide, acridine orange, thiazole orange, Coriphosphine-O, Fura 3, or fluorescein diacetate, you may want to test for residual stain after performing the routine cleaning procedure and before proceeding to your next...
  • Page 54 CLEANING PROCEDURES CLEAN THE SAMPLING SYSTEM MCL Sample Head and Sample Probe Cleaning Procedure Perform this procedure as often as described in the heading When to Clean the Sampling System. Prepare a cleaning solution of 1 part of high-quality, fragrance-free bleach b (5% or 6% solution of sodium hypochlorite - available chlorine) and 9 parts distilled water or IsoFlow...
  • Page 55 CLEANING PROCEDURES CLEAN THE SAMPLING SYSTEM Carefully push the moistened gauze pad up against the inside of the MCL sample head b and scrub away any debris inside and around the sample probe. Continue scrubbing the sample head and probe by pushing the head up and down 10 times during a 60-second period.
  • Page 56 CLEANING PROCEDURES CLEAN THE SAMPLING SYSTEM Vacuum Line Cleaning Procedure Perform this procedure as often as described in the heading When to Clean the Sampling System. Put 5 mL of distilled water into the fill reservoir of two cleaning adaptors. Load the cleaning adaptors into a carousel: b Put the cleaning adaptors into...
  • Page 57 CLEANING PROCEDURES CLEAN THE SAMPLING SYSTEM Put the carousel into the MCL and close the MCL cover. When the message Press Idle Mode button to Initialize appears at the bottom of the screen: The message Cleanse cycle in progress appears during the cleaning cycle. When the cleaning cycle is done, the message changes back to Press Idle Mode button to Initialize.
  • Page 58 CLEANING PROCEDURES CLEAN THE SAMPLING SYSTEM Remove the carousel. Close the MCL cover. Record that the vacuum line cleaning procedure was performed on the electronic Maintenance Log. Before running samples: to initialize the system. 1-46 PN 175572B...
  • Page 59 CLEANING PROCEDURES CLEAN THE MCL SAMPLE HEAD AND THE SAMPLE PROBE 1.12 CLEAN THE MCL SAMPLE HEAD AND THE SAMPLE PROBE Do this procedure weekly to remove any crystal or debris buildup. To see a video of this condition, click S Click S for an overview.
  • Page 60 CLEANING PROCEDURES CLEAN THE MCL SAMPLE HEAD AND THE SAMPLE PROBE Remove the Front Left Side Panel: b Unscrew the two bolts that attach the left side panel to the front frame. c Snap off the left side panel. Moisten a Q-tip with distilled water. 1-48 PN 175572B...
  • Page 61 CLEANING PROCEDURES CLEAN THE MCL SAMPLE HEAD AND THE SAMPLE PROBE Clean the top of the MCL sample head and the bottom of the sample probe holder. Replace the Front Left Side Panel: b Snap on the left side panel. c Screw in the two bolts to attach the left side panel to the front frame.
  • Page 62 CLEANING PROCEDURES CLEAN THE MCL SAMPLE HEAD AND THE SAMPLE PROBE WARNING Risk of operator injury. To prevent injury, hold your hands flat on the sides of the cover. Ensure your fingers are not behind the cover as you push down and in to close it. Close the Front Cover.
  • Page 63 CLEANING PROCEDURES POWER THE CYTOMETER ONLY ON/OFF 1.13 POWER THE CYTOMETER ONLY ON/OFF Use the procedures below if the instrument has not been fully shut down. Otherwise use these more detailed procedures located in the Instructions For Use manual: Use the Power the Computer and Cytometer ON procedure if you need to start up the instrument and computer from a fully shut down condition.
  • Page 64 CLEANING PROCEDURES POWER THE CYTOMETER ONLY ON/OFF Power the Cytometer and CXP Software ON Use this procedure if the computer is already on and you want to start the Cytometer and the CXP software. On the Windows desktop: to start the CXP software and power up the Cytometer.
  • Page 65 CLEANING PROCEDURES POWER THE CYTOMETER ONLY ON/OFF Power the Cytometer OFF Use this procedure to turn off the Cytometer and close the CXP software. ® You can still work with the Windows software after the Cytometer shuts off. in all open CXP windows. to turn off the Cytometer.
  • Page 66 CLEANING PROCEDURES POWER THE CYTOMETER ONLY ON/OFF 1-54 PN 175572B...

  • Page 67: Reset The Circuit

    REPLACE/ADJUST PROCEDURES WHAT THIS CHAPTER EXPLAINS List of Replacement and Adjustment Procedures This chapter has these replacement and adjustment procedures: REPLACE REAGENTS FILL THE SHEATH FLUID CONTAINER VENT THE AIR BUBBLES FILL THE CLEANING AGENT CONTAINER EMPTY THE WASTE CONTAINER REPLACE THE SHEATH FLUID FILTER REPLACE THE SAMPLE PROBE AND SAMPLE PICKUP TUBING REPLACE THE MCL SAMPLE HEAD...
  • Page 68 REPLACE/ADJUST PROCEDURES FILL THE SHEATH FLUID CONTAINER Reagent Container Capacity The sheath fluid container has a working capacity of about 2 L. This is the amount of reagent needed when you are filling the sheath fluid container after the indicator appears. Sheath Low When you fill a completely empty sheath fluid container (after cleaning or replacement), you need about 2.8 L of sheath fluid due to pressurization and level sensing requirements.
  • Page 69 REPLACE/ADJUST PROCEDURES FILL THE SHEATH FLUID CONTAINER Open the Front Cover. Pull open the reagent drawer until it stops. Locate the sheath fluid container b. PN 175572B...
  • Page 70 REPLACE/ADJUST PROCEDURES FILL THE SHEATH FLUID CONTAINER IMPORTANT Misleading results could occur if you contaminate the sheath fluid. Be careful not to contaminate the sheath fluid. Do not let your fingers, paper towels, or other objects touch the inside of the container or the inside of its cap. Remove the cap: b Unscrew the cap on the sheath fluid container.
  • Page 71 REPLACE/ADJUST PROCEDURES FILL THE SHEATH FLUID CONTAINER Carefully wipe up any spills. IMPORTANT Misleading results could occur if you analyze samples without the cap on the sheath container. Be sure to put the cap back on the sheath fluid container after you fill it. Screw the cap back on.
  • Page 72 REPLACE/ADJUST PROCEDURES VENT THE AIR BUBBLES Close the reagent drawer and perform VENT THE AIR BUBBLES procedure. VENT THE AIR BUBBLES Perform this procedure: After you fill the internal sheath fluid container After you replace the sheath fluid filter When you are troubleshooting and suspect bubles in the sheath fluid tubing.
  • Page 73 REPLACE/ADJUST PROCEDURES VENT THE AIR BUBBLES Procedure Proceed as follows: If you have just replaced the sheath fluid or sheath fluid filter, go to step 3. If you are troubleshooting a problem and you suspect bubbles in the sheath fluid tubing, go to step 2.
  • Page 74 REPLACE/ADJUST PROCEDURES VENT THE AIR BUBBLES Press and hold the vent button until the bubbles in the vent tubing b are gone. WARNING Risk of operator injury. To prevent injury, hold your hands flat on the sides of the cover. Ensure your fingers are not behind the cover as you push down and in to close it.
  • Page 75 REPLACE/ADJUST PROCEDURES FILL THE CLEANING AGENT CONTAINER FILL THE CLEANING AGENT CONTAINER Perform this procedure whenever Cleanse Level Warning or Cleanse Level Error appears. Check if the instrument is currently displaying the Idle mode: If yes (Press Idle Mode button to initialize appears), go to step 2.
  • Page 76 REPLACE/ADJUST PROCEDURES FILL THE CLEANING AGENT CONTAINER Pull open the reagent drawer until it stops. Locate the cleaning agent container b. IMPORTANT Misleading results could occur if you contaminate the cleaning agent. Be careful not to contaminate the cleaning agent. Do not let your fingers, paper towels, or other objects touch the inside of the container or the inside of its cap.
  • Page 77 REPLACE/ADJUST PROCEDURES FILL THE CLEANING AGENT CONTAINER CAUTION Risk of damage to the instrument if you overfill the cleaning agent container. Overfilling the cleaning agent container causes the cleaning agent to enter the pressurized line. Avoid spills. Do not tilt the container or remove it from the drawer to fill it.
  • Page 78 REPLACE/ADJUST PROCEDURES FILL THE CLEANING AGENT CONTAINER Screw the cap back on. Check that: The sheath filter vent port b is above the connector c. The tubing is not kinked or twisted. 2-12 PN 175572B...
  • Page 79 REPLACE/ADJUST PROCEDURES FILL THE CLEANING AGENT CONTAINER Close the reagent drawer. WARNING Risk of operator injury. To prevent injury, hold your hands flat on the sides of the cover. Ensure your fingers are not behind the cover as you push down and in to close it. Close the Front Cover.

  • Page 80: Procedure

    REPLACE/ADJUST PROCEDURES EMPTY THE WASTE CONTAINER EMPTY THE WASTE CONTAINER Instructions and graphics are given for both Power Supply configurations. Empty the waste container when: Waste Full indicator b appears, or Waste Level Warning or Waste Level Error appears. The 4-L waste b container sits in a bracket on the right side of the Power Supply. SYSTEM POPWER MCL POPWER ®...
  • Page 81 REPLACE/ADJUST PROCEDURES EMPTY THE WASTE CONTAINER Lift the waste container out of its bracket and swirl it before removing the cap. WARNING Risk of biohazardous contamination if you have skin contact with the waste container, its contents, and its associated tubing. The waste container and its associated tubing might contain residual biological material and must be handled with care.
  • Page 82 REPLACE/ADJUST PROCEDURES EMPTY THE WASTE CONTAINER Empty the waste container according to your laboratory's procedures. Note: Take proper precautions to avoid spills if you are emptying the waste container into a sink, drain, or larger container. Put about 400 mL of high-quality, fragrance-free bleach b (5% or 6% sodium hypochlorite - available chlorine) in the waste container to...
  • Page 83 REPLACE/ADJUST PROCEDURES EMPTY THE WASTE CONTAINER Replace the cap and securely tighten. Note: Properly dispose of the leakproof disposable container used in step after you screw the cap back on the waste container. Wipe the bracket with a paper towel moistened with a 10% bleach solution, and then return the container to the bracket.

  • Page 84: Procedure

    REPLACE/ADJUST PROCEDURES REPLACE THE SHEATH FLUID FILTER The system automatically performs an initialization cycle if you emptied the waste container after the Waste Full indicator appeared. Note: If you emptied the waste container before the Waste Full indicator appeared: to initialize the system. REPLACE THE SHEATH FLUID FILTER Replace the 0.2-µm sheath fluid filter b: Every 6 months.
  • Page 85 REPLACE/ADJUST PROCEDURES REPLACE THE SHEATH FLUID FILTER Open the Front Cover. Open the reagent drawer. Locate the sheath filter b. 2-19 PN 175572B...
  • Page 86 REPLACE/ADJUST PROCEDURES REPLACE THE SHEATH FLUID FILTER Undo the flexible strap holding the sheath fluid filter. CAUTION Risk of damage to the instrument if you do not install the sheath fluid filter correctly. It allows fluid to flow in one direction only. Make sure you install the new sheath fluid filter correctly.
  • Page 87 REPLACE/ADJUST PROCEDURES REPLACE THE SHEATH FLUID FILTER Discard the old sheath fluid filter. Wipe up any spills, and then put the filter in the drawer. Check that: The vent port b is above the connector c. The tubing is not kinked or twisted.

  • Page 88: Maintenance

    REPLACE/ADJUST PROCEDURES REPLACE THE SHEATH FLUID FILTER Close the reagent drawer and perform VENT THE AIR BUBBLES procedure. WARNING Risk of operator injury. To prevent injury, hold your hands flat on the sides of the cover. Ensure your fingers are not behind the cover as you push down and in to close it.
  • Page 89 REPLACE/ADJUST PROCEDURES REPLACE THE SAMPLE PROBE AND SAMPLE PICKUP TUBING REPLACE THE SAMPLE PROBE AND SAMPLE PICKUP TUBING Replace the sample probe and sample pickup tubing when: The sample probe is bent. To see a video of this condition, click S The sample probe leaks.
  • Page 90 REPLACE/ADJUST PROCEDURES REPLACE THE SAMPLE PROBE AND SAMPLE PICKUP TUBING Remove the Front Left Side Panel: b Unscrew the two screws that attach the left side panel to the front frame. c Snap off the left side panel. Unscrew the sample pickup tubing connector from the bottom of the flow cell compartment.
  • Page 91 REPLACE/ADJUST PROCEDURES REPLACE THE SAMPLE PROBE AND SAMPLE PICKUP TUBING Pull the sample pickup tubing out through the left (MCL) side of the instrument. Remove the clip from the sample probe. Retain the clip. 2-25 PN 175572B...
  • Page 92 REPLACE/ADJUST PROCEDURES REPLACE THE SAMPLE PROBE AND SAMPLE PICKUP TUBING Lift the sample probe up and out of its holder. WARNING Risk of biohazardous contamination if you have skin contact with the sample pickup tubing. The sample pickup tubing might contain residual biological material and must be handled with care.
  • Page 93 REPLACE/ADJUST PROCEDURES REPLACE THE SAMPLE PROBE AND SAMPLE PICKUP TUBING Ensure that the rubber washer b and O-ring c are positioned correctly on the new sample probe. To view how to install the sample probe and sample pickup tubing, click S Thread the sample pickup tubing through the instrument.
  • Page 94 REPLACE/ADJUST PROCEDURES REPLACE THE SAMPLE PROBE AND SAMPLE PICKUP TUBING Insert the new sample probe into the sample probe holder. Guide the sample probe tip into the MCL sample head. Insert the clip removed in step into the groove on the sample probe. 2-28 PN 175572B...
  • Page 95 REPLACE/ADJUST PROCEDURES REPLACE THE SAMPLE PROBE AND SAMPLE PICKUP TUBING IMPORTANT Risk of erroneous results if the flow cell is misaligned. Overtightening the connector from the sample pickup tubing to the flow cell can cause misalignment of the flow cell. Only screw on the sample pickup tubing connector “finger tight.”...
  • Page 96 REPLACE/ADJUST PROCEDURES REPLACE THE SAMPLE PROBE AND SAMPLE PICKUP TUBING WARNING Risk of operator injury. To prevent injury, hold your hands flat on the sides of the cover. Ensure your fingers are not behind the cover as you push down and in to close it. Close the Front Cover.
  • Page 97 REPLACE/ADJUST PROCEDURES REPLACE THE MCL SAMPLE HEAD After the prime cycle is done: again. 2.10 REPLACE THE MCL SAMPLE HEAD Use this procedure when: Liquid from the cleaning adaptors does not draw up properly. Cleaning the sample head does not fix your excessive carryover problem. Numerous Sample Pressure Error or MCL Tube Up/Down Error messages occur.
  • Page 98 REPLACE/ADJUST PROCEDURES REPLACE THE MCL SAMPLE HEAD Open the Front Cover. Remove the Front Left Side Panel: b Unscrew the two bolts that attach the left side panel to the front frame. c Snap off the left side panel. 2-32 PN 175572B...
  • Page 99 REPLACE/ADJUST PROCEDURES REPLACE THE MCL SAMPLE HEAD Use a 0.050 in. Allen wrench to loosen the side b and front c setscrews on the sample head. Pull off the sample head. 2-33 PN 175572B...
  • Page 100 REPLACE/ADJUST PROCEDURES REPLACE THE MCL SAMPLE HEAD Pull the sample head and tubing through the instrument behind the frame. Loosen the thumbscrew at the side of the pneumatic drawer. Pull out the pneumatic drawer so you can access the tubing manifold. Loosen the thumbscrew holding the upper tubing manifold.
  • Page 101 REPLACE/ADJUST PROCEDURES REPLACE THE MCL SAMPLE HEAD Pull off the tubing manifold. WARNING Risk of biohazardous contamination if you have skin contact with the sample head and its tubing. The sample head tubing might contain residual biological material and must be handled with care.
  • Page 102 REPLACE/ADJUST PROCEDURES REPLACE THE MCL SAMPLE HEAD Place the new tubing manifold into the bracket in the pneumatic drawer and tighten the thumbscrew. Note: You might find it easier to screw in the thumbscrew with a screwdriver. To view how to install the MCL sample head, click S Route the sample head through the instrument.
  • Page 103 REPLACE/ADJUST PROCEDURES REPLACE THE MCL SAMPLE HEAD Slide the pneumatic drawer in and tighten the thumbscrew. Position and hold the sample head up against its bracket. Tighten the side b setscrew first. Then tighten the front c setscrew. 2-37 PN 175572B...
  • Page 104 REPLACE/ADJUST PROCEDURES REPLACE THE MCL SAMPLE HEAD Replace the Front Left Side Panel: b Snap on the left side panel. c Screw in the two bolts to attach the left side panel to the front frame. WARNING Risk of operator injury. To prevent injury, hold your hands flat on the sides of the cover.
  • Page 105 REPLACE/ADJUST PROCEDURES REPLACE THE MCL SAMPLE HEAD Close the MCL cover. Power the Cytometer Only ON Power the Cytometer and CXP Software 2-39 PN 175572B...
  • Page 106 REPLACE/ADJUST PROCEDURES ADJUST THE SYSTEM PRESSURE 2.11 ADJUST THE SYSTEM PRESSURE Adjust the system pressure if the System Pressure gauge is not reading 30 ±2 psi. Daily Startup describes how to check the System Pressure gauge reading on the Power Supply.
  • Page 107 REPLACE/ADJUST PROCEDURES ADJUST THE SYSTEM PRESSURE Locate the Pressure Adjust knob b. FILTER FILTER WATER WATER TRAP TRAP TRAP TRAP SYSTEM POWER SYSTEM POWER FILTER FILTER MCL POWER MCL POWER PRESS PRESS PRESS PRESS Pull the collar around the Pressure Adjust knob out toward you.
  • Page 108 REPLACE/ADJUST PROCEDURES ADJUST THE SYSTEM PRESSURE Adjust the pressure to 30 ±2 psi. b To decrease, turn to the left. c To increase, turn to the right. Push in on the collar to lock it into place. 2-42 PN 175572B...
  • Page 109 REPLACE/ADJUST PROCEDURES REPLACE THE OPTICAL FILTER PLATE 2.12 REPLACE THE OPTICAL FILTER PLATE Replace the optical filter plate when you need to use a different filter configuration for a different application. Remove the Optical Filter Plate Click S for an overview. IMPORTANT Risk of incorrect readings from a contaminated filter if you wear gloves with powder to perform this procedure.
  • Page 110 REPLACE/ADJUST PROCEDURES REPLACE THE OPTICAL FILTER PLATE Pinch down on both release clips of the optical area cover. Slide the cover forward and lift it off. Use a 3/16 in. Allen wrench to unscrew the bolt in the middle of the optical filter plate.
  • Page 111 REPLACE/ADJUST PROCEDURES REPLACE THE OPTICAL FILTER PLATE Lift the optical filter plate up and off. Set this optical filter plate aside. 2-45 PN 175572B...
  • Page 112 REPLACE/ADJUST PROCEDURES REPLACE THE OPTICAL FILTER PLATE Install the Optical Filter Plate Click S for an overview. IMPORTANT Risk of incorrect readings from a contaminated filter if you wear gloves with powder to perform this procedure. Powder from the gloves can contaminate the filter and cause incorrect readings. Wear powder-free gloves whenever you are working with any optical filter components.
  • Page 113 REPLACE/ADJUST PROCEDURES REPLACE THE OPTICAL FILTER PLATE Use a 3/16 in. Allen wrench to screw in the bolt in the middle of the optical filter plate. IMPORTANT Risk of incorrect results. If the optical area cover is not replaced securely, there will be no fluorescence signals but there will be FS and SS signals.
  • Page 114 REPLACE/ADJUST PROCEDURES REPLACE A FILTER IN THE OPTICAL FILTER PLATE Power the Cytometer Only ON Power the Cytometer and CXP Software 2.13 REPLACE A FILTER IN THE OPTICAL FILTER PLATE Perform this procedure when: When there is a loss of signal power - replace the old filter with a new filter of the same type.
  • Page 115 REPLACE/ADJUST PROCEDURES REPLACE A FILTER IN THE OPTICAL FILTER PLATE Turn the optical filter plate on its side. To see a video presentation of the main steps of how to remove a filter, click On the underside of the optical filter plate, locate the screw that holds the filter holder to be removed.
  • Page 116 REPLACE/ADJUST PROCEDURES REPLACE A FILTER IN THE OPTICAL FILTER PLATE Use the special tool provided to loosen the metal ring on the filter holder. Note: You might find it easier to finish loosening the metal ring by turning it with your gloved fingers. Insert the tool into the metal ring’...
  • Page 117 REPLACE/ADJUST PROCEDURES REPLACE A FILTER IN THE OPTICAL FILTER PLATE Determine which is the coated side b of a non-BCI filter: Take the eraser end of a pencil and hold it close to the filter, near its edge. Look at the two reflections, dark- and light-colored, of the pencil.
  • Page 118 REPLACE/ADJUST PROCEDURES REPLACE A FILTER IN THE OPTICAL FILTER PLATE Insert the special tool into the metal ring’ s two slots and turn to the right to tighten. Note: You might find it easier to begin tightening the metal ring by turning it with your gloved fingers.
  • Page 119 REPLACE/ADJUST PROCEDURES REPLACE A FILTER IN THE OPTICAL FILTER PLATE Insert the filter holder into the optical filter plate and screw in the holding screw. Install the Optical Filter Plate into the instrument. To see a video presentation of the main steps of how to install the optical filter plate, click S Note: If you replaced a damaged filter...
  • Page 120 REPLACE/ADJUST PROCEDURES POSITION THE FIELD STOP 2.14 POSITION THE FIELD STOP You can change the angle of forward scatter (FS) light collection from 1-19° to 1-8° by putting the Field Stop into position. Put the Field Stop into position (1-8°) for better separation of cell populations in samples with a lot of debris.
  • Page 121 REPLACE/ADJUST PROCEDURES POSITION THE FIELD STOP To put the Field Stop into position and 1-8° use the 1-8° angle of FS light collection: Slide the knob all the way to the left end of its track. Push it in to lock it into position. To move the Field Stop so it is not in 1-19°...
  • Page 122 REPLACE/ADJUST PROCEDURES RESET THE CIRCUIT BREAKERS 2.15 RESET THE CIRCUIT BREAKERS Perform this procedure when: The unit does not go to Ready when you turn the power ON. There is no compressor sound from the Power Supply when there normally should be. Instructions and graphics are given for both Power Supply configurations.
  • Page 123 REPLACE/ADJUST PROCEDURES RESET THE CIRCUIT BREAKERS Procedure 1 Check the indicators b through the front door of the Power Supply. SYSTEM POPWER MCL POPWER Open the Power Supply front door. FILTER WATER TRAP TRAP SYSTEM POWER FILTER MCL POWER PRESS PRESS 2-57 PN 175572B...
  • Page 124 REPLACE/ADJUST PROCEDURES RESET THE CIRCUIT BREAKERS If the indicators are off (dark), reset the circuit breakers. Put the main switch in the 0 position to turn the power supply off. FILTER WATER TRAP TRAP SYSTEM POWER FILTER MCL POWER PRESS PRESS Reset the circuit breakers by pressing all 26 buttons...
  • Page 125 REPLACE/ADJUST PROCEDURES RESET THE CIRCUIT BREAKERS Put the main switch in the 1 position to turn the power supply back on. FILTER WATER TRAP TRAP SYSTEM POWER FILTER MCL POWER PRESS PRESS Power the Cytometer OFF and then Power the Cytometer and CXP Software 2-59 PN 175572B...
  • Page 126 REPLACE/ADJUST PROCEDURES RESET THE CIRCUIT BREAKERS Procedure 2 Open the Power Supply front door. FILTER WATER TRAP TRAP SYSTEM POWER FILTER MCL POWER PRESS PRESS Check the indicators b on the front of the Power Supply. If any indicator is off (dark), proceed to step to reset the appropriate circuit breaker.
  • Page 127 REPLACE/ADJUST PROCEDURES RESET THE CIRCUIT BREAKERS Find the circuit breaker that corresponds to the dark indicator: System Power Indicator Circuit Breaker Power Indicator Circuit Breaker 5 VOLTS MCL 5 VOLTS +15V 15 VOLTS +12V MCL 5 VOLTS -15V 15 VOLTS -12V MCL 5 VOLTS +24V...
  • Page 128 2.16 ADJUST THE HENE LASER Use this procedure to make minor adjustments only to the HeNe laser. Note: If your instrument has an optional red solid-state laser, call your Beckman Coulter Representative for any adjustments. Adjustments should be made when the FL4 mean channel values of the integral signal intensity: Vary more than ±5% from the Mean integral signal channel within 24 hours, or...
  • Page 129 REPLACE/ADJUST PROCEDURES ADJUST THE HENE LASER Place a sample tube containing Flow-Check 675 fluorospheres (or the same Flow-Check fluorospheres mixture that you used for your daily QC) into the carousel. Put the carousel into the MCL sample loader. Close the MCL cover. Enter the Carousel No .
  • Page 130 REPLACE/ADJUST PROCEDURES ADJUST THE HENE LASER Setup Mode on the Cytometer Control Acquisition Setup Tab. View the rectangular regions in the FL4 Lin/AUX (FL4 Peak) plot. When the HeNe laser is out of alignment, the fluorospheres population typically appears in the lower left portion of the plot.
  • Page 131 REPLACE/ADJUST PROCEDURES ADJUST THE HENE LASER Select and drag the Reference region to center it on the fluorospheres population. This is just a visual aid to reference the fluorospheres population location prior to adjustment. Open the cover to access the HeNe laser.
  • Page 132 REPLACE/ADJUST PROCEDURES ADJUST THE HENE LASER Turn the HeNe laser’ s front adjustment knob clockwise to move the fluorospheres population up and to the right. If the population starts to move down, turn the knob counterclockwise. See step for details. LASER RADIATION AVOID EXPOSURE TO BEAM CLASS 3B LASER PRODUCT (IEC)
  • Page 133 Note: If you cannot move the fluorospheres population into the Target region: Turn the HeNe laser’ s front adjustment knob to return the fluorospheres population into the Reference region. Call your Beckman Coulter representative. Close the cover over the HeNe laser. 2-67 PN 175572B...
  • Page 134 REPLACE/ADJUST PROCEDURES ADJUST THE HENE LASER Deselect Setup Mode on the Cytometer Control Acquisition Setup Tab. Acquisition stops when the count is reached. When the processing is done, remove the carousel. Close the MCL cover. Run the appropriate QC Flow-Check protocol before running samples.
  • Page 135 MAINTENANCE AND SERVICE LOGS INTRODUCTION The Report Generator contains two log screens you can use to record maintenance and service activities. Use the MAINTENANCE LOG to record daily and periodic maintenance. Use the SERVICE LOG to record service conditions noted and actions taken. MAINTENANCE LOG The Maintenance Log lists actions that need to be performed and how often they are needed.

  • Page 136: Menu Options

    MAINTENANCE AND SERVICE LOGS MAINTENANCE LOG Menu Options The following menu items are available. File Menu The File menu has 3 options. Print - Prints the Maintenance Log of the current month. performs the same function. Save - Saves a new entry on the Maintenance Log. performs the same function.
  • Page 137 MAINTENANCE AND SERVICE LOGS MAINTENANCE LOG To enter a comment on any of the boxes, right-click on the box and select Insert Comment The presence of a comment in a box is indicated by a small red square in the upper right-hand corner of the box and is visible on the printout.
  • Page 138 MAINTENANCE AND SERVICE LOGS MAINTENANCE LOG The Administrator can add a task to the bottom of the fixed list by clicking on one of the empty rows. The task is added to the section starting with the current month. A total number of 5 additional task rows are available.

  • Page 139: Service Log

    MAINTENANCE AND SERVICE LOGS SERVICE LOG SERVICE LOG Date that the Condition Noted entry was made. The instrument serial number and facility name are displayed at the top. User who made the entry. This field is non-editable and it is filled in automatically by the system when the Date is entered.
  • Page 140 MAINTENANCE AND SERVICE LOGS SERVICE LOG Menu Options The following menu items are available. File Menu The File menu has 3 options. Print - Prints the Service Log of the current month. performs the same function. Save - Saves a new entry on the Service Log. performs the same function.
  • Page 141 MAINTENANCE AND SERVICE LOGS SERVICE LOG Double-click the Action Taken field and type the text describing the action taken. to save the Service Log. Start Date: and End Date: Use these fields to display the entries between Start Date and the End Date. The log displays the time period selected.
  • Page 142 MAINTENANCE AND SERVICE LOGS SERVICE LOG PN 175572B...
  • Page 143 INDEX Numerics cleaning schedule, 6 month procedures cleaning adaptor replace the sheath fluid filter, 2-18 how to use, 1-44 See also schedule illustration, 1-44 60 day procedures cleaning agent clean the cleaning agent container, 1-25 container, cleaning, 1-25 See also schedule container, when to clean, 1-25 filling the container,...
  • Page 144 INDEX conventions when to use, 2-54 used in the manual, filling Coriphosphine-O cleaning agent container, how to clean after using, 1-37 sheath fluid container, test for residual stain, 1-41 filter plate COULTER CLENZ See optical filter plate See cleaning agent filters covers air, cleaning,...
  • Page 145 INDEX indicators circuit breaker, chart, 2-57, 2-60 open circuit breaker, location, 2-57, 2-60 front cover, 1-11 sheath fluid low, left side panel, 1-15 waste full, 2-14 MCL lid, 1-17 instrument optical area covers, how to open, 1-11 cover, how to remove, 2-44 introduction to your manuals, how to access,...
  • Page 146 INDEX test for residual stain, 1-41 how to replace, 2-23 protocols when to replace, 2-23 HeNe alignment, 2-62 sample probe bent, 2-23 cleaning, daily, 1-42 cleaning. weekly, 1-47 reagent containers clip, location, 2-25 about, how to replace, 2-23 capacity, leaking, 2-23 filling with cleaning agent, no or erratic sample flow,...
  • Page 147 INDEX information organization, add bleach, 2-16 using the manual, capacity, 2-14 special tool emptying, 2-14 used to replace optical filter, 2-50 location, 2-14 stains waste full indicator, 2-14 residual, from old cleaning solution, 1-38 waste full indicator, 2-14 residual, testing for, 1-41 weekly procedures vital, how to clean after using,...
  • Page 148 INDEX INDEX-6 PN 175572B...
  • Page 149 TRADEMARKS The BECKMAN COULTER logo, COULTER CLENZ, CYTO-TROL, Immuno-Trol, and IsoFlow are trademarks of Beckman Coulter, Inc. All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders. PN 175572B...
  • Page 150 Cytomics FC 500 with CXP Software Documentation Reference Use and Function • Installation • Specifications • Log Sheets • Using PN 175570 CXP Software • Glossary • Index Special Procedures Cleaning Procedures • Replace/Adjust Procedures • Maintenance Log PN 175572 •...

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