Beckman Coulter UniCel DxC 600 Instructions For Use Manual page 82

System Description
Cartridge Chemistry: Calibration Theory
Table 1.17 Calculation of Calibration Factors for Endpoint and Rate Chemistries (Continued)
Type
Blanked Endpoint
Chemistries
(with Volume
Correction)
1-46
FOR HIGH CALIBRATOR LEVEL:
Blank Correction Factor =
Volume of Reagent(s) (and Sample) at Blank Read
=
Volume of Total Reagent and Sample at Reaction Read
[Reaction ABS - (Blank ABS × Blank Correction Factor)] = Delta ABS
[Reaction ABS - (Blank ABS × Blank Correction Factor)] = Delta ABS
(Delta ABS + Delta ABS
rep1 rep2
FOR LOW CALIBRATOR LEVEL:
[Reaction ABS - (Blank ABS × Blank Correction Factor)] = Delta ABS
[Reaction ABS - (Blank ABS × Blank Correction Factor)] = Delta ABS
(Delta ABS + Delta ABS
rep1 rep2
Cal Set Point (hi) – Cal Set Point (lo)
Cal Factor (Slope) =
Delta ABS
Offset = Cal Set Point (hi) - [Cal Factor × Delta ABS
Sample values are calculated by the following equation:
[[Reaction ABS - (Blank ABS × Blank Correction Factor)] × Cal Factor]
+ offset = sample value
IMPORTANT
For single point linear calibration, the low calibrator is a fixed zero point
and the offset is equal to zero.
Formula
Blank Volume
Total Reaction Volume
) × 0.5 = Delta ABS (hi)
avg
) × 0.5 = Delta ABS (lo)
avg
(hi) – Delta ABS
avg
(hi)]
avg
E007123L.EPS
rep1
rep2
rep1
rep2
(lo)
avg
E014417L.EPS
A13914AF