Beckman Coulter UniCel DxC 600 Instructions For Use Manual page 83

Table 1.17 Calculation of Calibration Factors for Endpoint and Rate Chemistries (Continued)
Type
Nonblanked Rate
Chemistries
A13914AF
FOR HIGH CALIBRATOR LEVEL:
Reaction Rate = Rate
rep1
Reaction Rate = Rate
rep2
(Rate + Rate ) × 0.5 = Rate
rep1 rep2
FOR LOW CALIBRATOR LEVEL:
Reaction Rate = Rate
rep1
Reaction Rate = Rate
rep2
(Rate + Rate ) × 0.5 = Rate
rep1 rep2
Cal Set Point (hi) – Cal Set Point (lo)
Cal Factor (Slope) =
Offset = Cal Set Point (hi) - [Cal Factor × Rate
Sample values are calculated by the following equation:
(Reaction Rate × Cal Factor) + offset = sample value
IMPORTANT
For single point linear calibration, the low calibrator is a fixed zero point
and the offset is equal to zero.
Cartridge Chemistry: Calibration Theory
Formula
(hi)
avg
(lo)
avg
Rate (hi) – Rate (lo)
avg avg
(hi)]
avg
System Description
E014418L.EPS
1-47
1