5 Service and settings
5.4 UV Monitor
5.4.1 UV LED calibration
5.4.1
UV LED calibration
Description
UV LED calibration is used for calibrating the UV LED intensity to get the desired response
level of the photo detector.
Parameter
Light
T amb
Signal
Instructions
Follow the instructions below to calibrate the UV LED.
Step
1
2
3
64
Description
Light intensity needed to get the expected response at the
photo detector.
UV temperature sensor reading.
The output of the photo detector at any time (mV).
Action
Make sure that:
The UV flow cell is assembled tightly.
•
The inlet and outlet tubing and the fingertight connectors are properly
•
tightened to block any stray light from entering the UV flow cell.
The system is not exposed to direct sunlight.
•
Flush the UV flow cell with demineralized water using the Pump. Make sure
that there are no air bubbles in the UV flow cell.
Tap UV LED calibration.
Result: The following screen opens.
ÄKTA start Maintenance Manual 29-0603-08 AC
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