GE akta start Maintenance Manual
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GE akta start Maintenance Manual

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Maintenance Manual
Original instructions

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Summary of Contents for GE akta start

  • Page 1 ÄKTA ™ start Maintenance Manual Original instructions...
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  • Page 3: Table Of Contents

    Table of Contents Table of Contents Introduction ......................About this manual ..........................Important user information ......................Regulatory information ........................Associated documentation ......................Safety instructions ....................Safety precautions ..........................Labels ................................. Emergency procedures ........................Recycling procedures ......................... Declaration of Hazardous Substances (DoHS) ................ System description ....................
  • Page 4 Table of Contents 5.12 Frac30 ................................ 5.12.1 Enable or disable Frac30 ......................5.12.2 Diagnostics ............................5.12.3 Run Log .............................. 5.13 Instrument Display ..........................5.13.1 Touch screen calibration ......................5.13.2 Color test ............................5.13.3 Diagnostics ............................5.13.4 Log book ............................5.14 System ...............................

  • Page 5: Introduction

    1 Introduction Introduction About this chapter This chapter contains important user information, description of safety notices, regula- tory information, intended use of ÄKTA start, and list of associated documentation. In this chapter Section See page 1.1 About this manual 1.2 Important user information 1.3 Regulatory information 1.4 Associated documentation ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 6: About This Manual

    1 Introduction 1.1 About this manual About this manual Purpose of this document The ÄKTA start Maintenance Manual provides you with the instructions needed to unpack, maintain and troubleshoot ÄKTA start in a safe way. Nomenclature conventions The nomenclature used in this manual are explained in the table below. Concept Explanation ÄKTA start...

  • Page 7: Important User Information

    1 Introduction 1.2 Important user information Important user information Read this before operating the product All users must read the entire Operating Instructions before installing, operating or maintaining the product. Always keep the Operating Instructions at hand when operating the product. Do not operate the product in any other way than described in the user documentation.
  • Page 8 1 Introduction 1.2 Important user information Safety notices This user documentation contains safety notices (WARNING, CAUTION, and NOTICE) concerning the safe use of the product. See definitions below. WARNING WARNING indicates a hazardous situation which, if not avoided, could result in death or serious injury. It is important not to proceed until all stated conditions are met and clearly understood.

  • Page 9: Regulatory Information

    EC Declaration of Conformity (DoC) document. Requirement Content Name and address of manufacturer GE Healthcare Bio-Sciences AB, Björkgatan 30, SE 751 84 Uppsala, Sweden Conformity with EU Directives This product complies with the European directives listed in the table below, by fulfilling the corresponding harmonized standards.

  • Page 10: Regulatory Information

    • connected to other products recommended or described in the user documentation, • used in the same state as it was delivered from GE, except for alterations described in the user documentation. International standards This product fulfills the requirements of the following standards:...

  • Page 11: Fcc Statement

    1 Introduction 1.3 Regulatory information FCC statement ÄKTA start complies with FCC 47 CFR Part 15b. Note: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.

  • Page 12: Associated Documentation

    Introduction This section describes the user documentation that is delivered with the instrument and how to find related literature that can be downloaded or ordered from GE. User documentation on the CD The user documentation listed in the table below is available on the ÄKTA start User Documentation CD.
  • Page 13 1 Introduction 1.4 Associated documentation From the Help menu in UNICORN start or on the UNICORN start DVD, the following user documentation is available. Documentation Main contents UNICORN start 1.0 User Manual Overview and detailed descriptions of the system control software designed for ÄKTA start, which includes process pic- ture map for real time monitoring, method editor, evaluation and adminis-...
  • Page 14 1 Introduction 1.4 Associated documentation Access documentation from mobile units Scan the code using your mobile phone or tablet computer to access the product page for ÄKTA start. Select documents to download under the Related Documents tab. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 15: Safety Instructions

    2 Safety instructions Safety instructions About this chapter This chapter describes safety precautions and emergency shutdown procedures for the product. The labels on the system and recycling procedures are also described. In this chapter This chapter contains the following sections: Section See page 2.1 Safety precautions...

  • Page 16: Safety Precautions

    2 Safety instructions 2.1 Safety precautions Safety precautions Introduction ÄKTA start is powered by mains voltage and handles liquids that may be hazardous. Before installing, operating or maintaining the system, you must be aware of the hazards described in this manual. Follow the instructions provided to avoid personal injuries or damage to the equipment.
  • Page 17 2 Safety instructions 2.1 Safety precautions Using flammable liquids WARNING When using flammable liquids with ÄKTA start, follow these precau- tions to avoid any risk of fire or explosion. • Fire Hazard. Before starting the system, make sure that there is no unintentional leakage in the instrument or tubing.
  • Page 18 2 Safety instructions 2.1 Safety precautions CAUTION To avoid hazardous situations when working with ÄKTA start, take the following measures for personal protection. • Always use appropriate personal protective equipment during operation and maintenance of ÄKTA start. • Spillage hazard. When using ÄKTA start use personal protective equipment like goggles, lab coat, protective shoes and gloves to avoid any circumstances of spillage.
  • Page 19 • Power cord. Only use grounded power cords delivered or ap- proved by GE. • Access to power switch and power cord with plug. Do not block access to the power switch and power cord. The power switch must always be easy to access.
  • Page 20 2 Safety instructions 2.1 Safety precautions NOTICE To avoid damage to ÄKTA start or other equipment when installing or moving the instrument, follow the instructions below. • Vents on ÄKTA start. To ensure adequate ventilation, keep papers and other objects away from the vents of the instru- ment.
  • Page 21 2 Safety instructions 2.1 Safety precautions CAUTION To avoid personal injury when operating ÄKTA start, follow the in- structions below. • Max weight on Buffer tray. Do not place containers with a volume of more than 1 liter each on the Buffer tray. The maxi- mum allowed weight on the Buffer tray is 5 kg.
  • Page 22 GE. • Only spare parts and accessories that are approved or supplied by GE may be used for maintaining or servicing ÄKTA start. • Disconnect power. Always switch off power to the instrument before replacing any component on the instrument or cleaning the instrument, unless stated otherwise in the user documen- tation.
  • Page 23 2 Safety instructions 2.1 Safety precautions NOTICE Cleaning. Keep the instrument dry and clean. Wipe regularly with a soft damp tissue and, if necessary, a mild cleaning agent. Let the instrument dry completely before use. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 24: Labels

    2 Safety instructions 2.2 Labels Labels Introduction This section describes the safety labels and labels concerning hazardous substances that are attached to ÄKTA start. The instrument serial number is also visible from the instrument product label which is illustrated here. Labels on ÄKTA start The illustrations below show the labels that are attached to ÄKTA start.
  • Page 25 2 Safety instructions 2.2 Labels Label Description Placement This product does not comply with the ATEX directive and must not be used in explosive atmo- spheres. Pinch hazard. Switch off the Pump before tubing loading. Label on Frac30 The illustration below shows the labels that are attached to Frac30. Label Description Placement...

  • Page 26: Safety Symbols

    2 Safety instructions 2.2 Labels Safety symbols The following safety symbols are used in the labels: Label Meaning Warning! Do not use ÄKTA start before reading the ÄKTA start Operating Instructions. Do not open any covers or re- place parts unless specifically stated in the user docu- mentation.
  • Page 27 2 Safety instructions 2.2 Labels Label Meaning This symbol indicates that the product contains haz- ardous materials in excess of the limits established by the Chinese standard SJ/T11363-2006 Requirements for Concentration Limits for Certain Hazardous Sub- stances in Electronic Information Products. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 28: Emergency Procedures

    2 Safety instructions 2.3 Emergency procedures Emergency procedures Introduction This section describes how to do an emergency shutdown of ÄKTA start. The section also describes the result in the event of power failure. Emergency shutdown In an emergency situation: Switch off power to the instrument by pressing the power switch to the O position or by disconnecting the power cord from the instrument.
  • Page 29 2 Safety instructions 2.3 Emergency procedures Power failure The result of a power failure depends on which unit is affected. Power failure to... will result in... ÄKTA start The run is interrupted immediately. • The data collected up to the time of the power fail- •...

  • Page 30: Recycling Procedures

    Recycling of hazardous substances ÄKTA start contains hazardous substances. Detailed information is available from your GE representative. Disposal of electrical components Waste comprising electrical and electronic equipment must not be disposed of as un- sorted municipal waste and must be collected separately.

  • Page 31: Declaration Of Hazardous Substances (Dohs)

    2 Safety instructions 2.5 Declaration of Hazardous Substances (DoHS) Declaration of Hazardous Substances (DoHS) Introduction The following product pollution control information is provided according to SJ/T11364- 2006 Marking for Control of Pollution caused by Electronic Information Products. 根据SJ/T11364-2006《电子信息产品污染控制标识要求》特提供如下有关污染 控制 方面的信息 Symbols used in pollution control label 电子信息产品污染控制标志说明...
  • Page 32 2 Safety instructions 2.5 Declaration of Hazardous Substances (DoHS) Label Meaning 该标志表明本产品含有超过SJ/T11363-2006《电子信息产品中有毒 有害物质的限 量要求》中限量的有毒有害物质。标志中的数字为本 产品的环保使用期，表明本 产品在正常使用的条件下，有毒有害物 质不会发生外泄或突变，用户使用本产品 不会对环境造成严重污染 或对其人身、财产造成严重损害的期限。单位为年。 为保证所申明的环保使用期限，应按产品手册中所规定的环境条件 和方法进行正 常使用，并严格遵守产品维修手册中规定的期维修和 保养要求。 产品中的消耗件和某些零部件可能有其单独的环保使用期限标志， 并且其环保使 用期限有可能比整个产品本身的环保使用期限短。应 到期按产品维修程序更换那 些消耗件和零部件，以保证所申明的整 个产品的环保使用期限。 本产品在使用寿命结束时不可作为普通生活垃圾处理，应被单独收 集妥善处理 List of hazardous substances and their concentrations 产品中有毒有害物质或元素的名称及含量 Indication for each major part if substance exceeds limit Value Meaning Indicates that this toxic or hazardous substance contained in all of the...
  • Page 33 2 Safety instructions 2.5 Declaration of Hazardous Substances (DoHS) List of hazardous substances Component Hazardous substance name 有毒有害物质或元素 部件名称 Cr6+ PBDE 铅 汞 镉 六价铬 多溴联苯 多溴二苯醚 ÄKTA start, 29-0220-94 Frac30, 29-0230-51 UNICORN start, 29-0187-51 The product has not been tested as per the Chinese standard SJ/T11363-2006 Requirements for Concentration Limits for Certain Hazardous Substances in Electronic Information Product.

  • Page 34: System Description

    3 System description System description About this chapter This chapter provides an overview of ÄKTA start, and the optional Frac30 (Fraction col- lector). For details about the Instrument Display, refer to ÄKTA start Operating Instructions. In this chapter This chapter contains the following section: Section See page 3.1 System overview...

  • Page 35: System Overview

    3 System description 3.1 System overview System overview Introduction ÄKTA start is the main instrument included in a liquid chromatography system intended primarily for preparative purification of proteins at laboratory-scale. The system can be used for a variety of research purposes to fulfill the needs of the users in the academia and in life sciences industry.
  • Page 36 3 System description 3.1 System overview Illustration of the instrument The illustration below shows the main parts of the instrument. Part Description Function Instrument Display User interface for controlling the system and visualization of the runtime data. Wet side The modules interconnected by tubing have the following functions: to deliver the liquid in a specified flow path •...
  • Page 37 3 System description 3.1 System overview Illustration of the Fraction collector The illustration below shows the Fraction collector; Frac30. Part Description Function Dispenser arm Holds and positions the tubing holder for dispens- assembly ing the liquid into fractions. Tubing holder Holds the tubing used for dispensing the liquid fractions into the collection tubes.
  • Page 38 3 System description 3.1 System overview Main features of ÄKTA start The main features of ÄKTA start are listed below: • ÄKTA start is a compact and one step purification solution for quick and reliable pu- rification of proteins. • A simple and modern system offered to automate the protein purification workflow by providing features like automated sample injection, fraction collection, real-time monitoring.

  • Page 39: Instrument

    3 System description 3.2 Instrument Instrument Introduction This section provides an overview of ÄKTA start. Illustration of the instrument modules The illustration below shows the locations and gives brief descriptions of the modules placed on the wet side of the instrument. Part Function Description...
  • Page 40 3 System description 3.2 Instrument Part Function Description Mixer A static mixer that is used for mixing of buffers A and B. Sample valve A 3-port valve that allows either the buffer or the sample to enter the flow path. The Sample valve enables direct application of the sample onto the column using the Pump.
  • Page 41 3 System description 3.2 Instrument Part Function Description Conductivity The Conductivity Monitor continuously reads the conductivity of the liquid in the Conductivity flow cell. The conductivity is automatically calculated by multiplying the measured conductance by the cell constant of the flow cell. The cell constant is factory-calibrated.

  • Page 42: Unpack Äkta Start And Frac30

    4 Unpack ÄKTA start and Frac30 Unpack ÄKTA start and Frac30 About this chapter This chapter provides the necessary instructions to unpack ÄKTA start. Read the entire Installation chapter in ÄKTA start Operating Instructions, before starting to install ÄKTA start. In this chapter This chapter contains the following sections: Section...

  • Page 43: Unpack Äkta Start

    4 Unpack ÄKTA start and Frac30 4.1 Unpack ÄKTA start Unpack ÄKTA start Instruction Follow the instructions below to unpack the instrument. CAUTION Take care not to damage any module or the capillary tubing when lifting the instrument or when removing the plastic bag. CAUTION ÄKTA start is filled with denaturated alcohol (50% C2H5OH (ethanol)) at delivery.
  • Page 44 4 Unpack ÄKTA start and Frac30 4.1 Unpack ÄKTA start Step Action Take out the document placed at the top of the package and read the Un- packing Instructions. Note: Save the documents for future reference. Take out the box placed at the top of the package. The box contains the accessories delivered with the instrument.
  • Page 45 4 Unpack ÄKTA start and Frac30 4.1 Unpack ÄKTA start Step Action Hold the red strap, and then lift the instrument out of the delivery box. Open the strap lock and remove the strap. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 46 4 Unpack ÄKTA start and Frac30 4.1 Unpack ÄKTA start Step Action Remove the foam cushion from the top of the instrument. Remove the foam cushion from the bottom of the instrument by carefully lifting the instrument. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 47 4 Unpack ÄKTA start and Frac30 4.1 Unpack ÄKTA start Step Action Remove the plastic bag by gently tilting the system back and forth while pulling out the plastic bag. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 48: Unpack Frac30

    4 Unpack ÄKTA start and Frac30 4.2 Unpack Frac30 Unpack Frac30 Instruction Follow the instructions below to unpack the Fraction collector. CAUTION Take care not to damage the Dispenser arm when lifting Frac30 or when removing the plastic bag. NOTICE Never lift the Fraction collector Frac30 by the Dispenser arm.
  • Page 49 4 Unpack ÄKTA start and Frac30 4.2 Unpack Frac30 Step Action Holding the red strap, lift the fraction collector out of the delivery box. Place the Fraction collector on the laboratory bench. Open the strap lock and remove the strap. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 50 4 Unpack ÄKTA start and Frac30 4.2 Unpack Frac30 Step Action Remove the foam cushion from the top of the Fraction collector. Remove the foam cushion from the bottom of the fraction collector by carefully lifting the Fraction collector. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 51 4 Unpack ÄKTA start and Frac30 4.2 Unpack Frac30 Step Action Remove the plastic bag. Remove the Bowl assembly from the Base unit: Gently move the Dispenser arm counterclockwise to the end position. • Push the Drive assembly and hold it at the retracted position. At the •...
  • Page 52 4 Unpack ÄKTA start and Frac30 4.2 Unpack Frac30 Step Action Remove the foam cushion located on the Base unit. Re-assemble the Bowl assembly on to the Base unit: Orient the Bowl to match the aligning groove and the aligning features •...
  • Page 53 4 Unpack ÄKTA start and Frac30 4.2 Unpack Frac30 NOTICE Never use the Dispenser arm assembly to lift or hold Frac30. To lift the module, use the handle provided on the bottom plate. Note: Do not damage or break the warranty seal label during unpacking of Frac30. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 54: Accessories Package

    4 Unpack ÄKTA start and Frac30 4.3 Accessories package Accessories package Illustration of the accessories package The illustration below shows the accessories box and the user documentation included with ÄKTA start at delivery. ÄKTA start Part Description Unpacking Instructions System certificate Product documentation Maintenance Cue Card System Cue Card...

  • Page 55: Service And Settings

    5 Service and settings Service and settings About this chapter Regular maintenance of ÄKTA start is required to ensure safe operation and optimal performance. This chapter provides instructions for periodic maintenance, including calibration and configuration, as well as other required maintenance. In this chapter This chapter contains the following sections: Section...

  • Page 56: Preventive Maintenance

    5 Service and settings 5.1 Preventive maintenance Preventive maintenance Introduction Preventive maintenance should be performed on a daily, weekly and monthly basis. For cleaning instructions, refer to ÄKTA start Operating Instructions. Preventive maintenance schedule Interval Maintenance action Daily Visually inspect the instrument for leakages in the flow path. Check the Pump for leakage.
  • Page 57 5 Service and settings 5.1 Preventive maintenance Interval Maintenance action When required Clean the Fraction collector. Note: Frac30 is not a spill free design. There will be some spillage when tubes shift position, depending on the flow rate. Yearly, or when re- Visually inspect the drive sleeve on the Fraction collector.

  • Page 58: Clean Before Planned Maintenance Or Service

    Please complete the checklist in the On Site Service Health & Safety Declaration Form or the Health & Safety Declaration Form for Product Return or Servicing, depending on whether the instrument is going to be serviced on site or returned to GE for service, re- spectively. Copy the form you need from Section 8.1 Health and Safety Declaration Form,...

  • Page 59: Access The Modules

    5 Service and settings 5.3 Access the modules Access the modules Illustration of the modules The illustration below shows the locations of the modules placed on the wet side of the instrument. Part Module Service & settings instructions Buffer valve Instructions, on page 82 Mixer Sample valve...
  • Page 60 5 Service and settings 5.3 Access the modules Part Module Service & settings instructions Section 5.4 UV Monitor, on page 62 Conductivity Instructions, on page 95 Instructions, on page 97 Instructions, on page 98 Outlet valve Instructions, on page 91 Instructions Maintenance and service of the different modules are operated from the Instrument Display.
  • Page 61 5 Service and settings 5.3 Access the modules Step Action Tap Next or Back to move through the screens 1 to 3. Tap the intended module button to select a specific module for maintenance. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 62: Uv Monitor

    5 Service and settings 5.4 UV Monitor UV Monitor Introduction This section describes how to access the UV Monitor options, perform calibrations and edit UV settings. In this section This section contains the following subsections: Section See page 5.4.1 UV LED calibration 5.4.2 Diagnostics 5.4.3 Flow cell path length 5.4.4 Reset number of run hours...
  • Page 63 5 Service and settings 5.4 UV Monitor Step Action Tap UV to access the UV Monitor options. Result: The UV screen opens. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 64: Uv Led Calibration

    5 Service and settings 5.4 UV Monitor 5.4.1 UV LED calibration 5.4.1 UV LED calibration Description UV LED calibration is used for calibrating the UV LED intensity to get the desired response level of the photo detector. Parameter Description Light Light intensity needed to get the expected response at the photo detector.
  • Page 65 5 Service and settings 5.4 UV Monitor 5.4.1 UV LED calibration Step Action Set the Light strength value to 500 using up/down arrows or enter the value. Tap Calibrate. Result: A confirmation screen opens. Tap OK to automatically adjust the Light strength value to get a minimum Signal response of 2500 mV.

  • Page 66: Diagnostics

    5 Service and settings 5.4 UV Monitor 5.4.2 Diagnostics 5.4.2 Diagnostics Description Diagnostics is used to conduct a Dark current test and/or a Stray light test to assess any electrical noise or presence of stray light. Test Description Dark current test Switches off the UV LED during the test.
  • Page 67 5 Service and settings 5.4 UV Monitor 5.4.2 Diagnostics Step Action Tap Diagnostics. Result: The Diagnostics screen opens. Tap Set baseline to capture a new reference value for the test. Tap Dark current test. The result is displayed as Pass or Fail: Pass: the UV Monitor is working as it should.
  • Page 68 Make sure that the detector is not exposed to e.g., direct sunlight. If the test fails again, replace the flow cell and then possibly the UV Monitor, or contact a GE Service Engineer. Flush the system with demineralized water so that the Abs value returns close to zero.

  • Page 69: Flow Cell Path Length

    C drft UV LED drift compensation constant. NOTICE UV Configuration of C amb and C drft values must be performed by a GE Service Engineer. The C amb and C drft values should not be altered by a user. Prerequisites •...
  • Page 70 5 Service and settings 5.4 UV Monitor 5.4.3 Flow cell path length Instructions Follow the instructions below to perform a Flow cell path length test and set a new cell length. Step Action Flush the UV flow cell with demineralized water using the Pump, and then leave the UV flow cell filled with water.
  • Page 71 5 Service and settings 5.4 UV Monitor 5.4.3 Flow cell path length Step Action Update the calculated Cell length value by using up/down buttons or enter the value. Tap Save path length to save the value to permanent memory. Note: The Abs value should be 340 ±...

  • Page 72: Reset Number Of Run Hours

    Follow the instructions below to reset the number of run hours of UV Monitor. NOTICE UV Configuration of C amb and C drft values must be performed by a GE Service Engineer. The C amb and C drft values should not be altered by a user. Note: After replacing the UV Monitor, it is required to reset the number of run hours of UV Monitor.
  • Page 73 5 Service and settings 5.4 UV Monitor 5.4.4 Reset number of run hours Step Action Tap Reset. Result: A confirmation screen opens If the UV Monitor has been replaced, tap Yes. If the UV Monitor has not been replaced, tap No to cancel the action. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 74: Pump

    5 Service and settings 5.5 Pump Pump Introduction This section describes how to perform pump diagnostics, calibrations and handle the pump tubing log. In this section This section contains the following sub-sections: Section See page 5.5.1 Diagnostics 5.5.2 Calibration 5.5.3 Pump tubing log Access the Pump service options Follow the instruction below to access the options for Pump calibration and troubleshoot- ing.
  • Page 75 5 Service and settings 5.5 Pump Step Action Tap to select the intended option. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 76: Diagnostics

    5 Service and settings 5.5 Pump 5.5.1 Diagnostics 5.5.1 Diagnostics Parameter description Parameter Description Flow rate Setting of the desired flow rate (ml/min). Pump run Displays the actual number of run hours for the Pump. Check the Pump flow rate Follow the instructions below to perform Pump diagnostics.
  • Page 77 5 Service and settings 5.5 Pump 5.5.1 Diagnostics Step Action Prime the Pump and the flow path. Tap Start flow to run the Pump. • Place the outlet tubing from the Wash valve (Waste port) in a pre- • weighed collection tube. Note: Before starting Diagnostics, prime the flow path with water and make sure that the outlet tubing where the pumped water is collected, is filled with...

  • Page 78: Calibration

    5 Service and settings 5.5 Pump 5.5.2 Calibration 5.5.2 Calibration Parameter description Parameter Description Flow Rate The intended flow rate used for Pump calibration. Collected Volume The volume of liquid collected for a certain period of time at the set flow rate. The Collected Volume value and the expected value of the volume corresponding to the set flow rate are used internally for calibration.
  • Page 79 5 Service and settings 5.5 Pump 5.5.2 Calibration Step Action Use the up/down arrows to set the desired Flow Rate. Tap Start flow to start the Pump. • Collect water for at least one minute in the pre-weighed collection tube. •...

  • Page 80: Pump Tubing Log

    5 Service and settings 5.5 Pump 5.5.3 Pump tubing log 5.5.3 Pump tubing log Parameter description Parameter Description Tubing run Indicates how many hours the pump tubing has been • used. When the pump tubing is replaced, use the Reset but- •...
  • Page 81 5 Service and settings 5.5 Pump 5.5.3 Pump tubing log Step Action Tap Yes if the pump tubing has been replaced. If the pump tubing has not been replaced, tap No to cancel the action. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 82: Buffer Valve

    5 Service and settings 5.6 Buffer valve Buffer valve Description The Buffer valve can be switched to allow the inlet of either buffer A or buffer B, or a mixture of A and B (gradient). Parameter Description Valve switches Indicates how many times the valve has switched be- •...
  • Page 83 5 Service and settings 5.6 Buffer valve Step Action In the Settings and service screen, tap Next to access the 2 screen. Result: The following screen opens. In the Settings and service screen, tap Buffer valve. Result: The Buffer valve screen opens. Check that the valve position Buffer A is selected.
  • Page 84 5 Service and settings 5.6 Buffer valve Step Action If these checks indicate that the valve is faulty, replace the Buffer valve. If the valve has been replaced, tap Reset to set the Valve switches counter to 0. Result: A confirmation screen opens. Tap Yes to confirm the reset if the valve has been replaced.

  • Page 85: Sample Valve

    5 Service and settings 5.7 Sample valve Sample valve Description The Sample valve can be switched to allow the inlet of either buffer or sample. Parameter Description Valve switches Indicates how many times the valve has switched. • When an old valve is defective and replaced, the •...
  • Page 86 5 Service and settings 5.7 Sample valve Step Action In the Settings and service screen, tap Next to access the 2 screen. Result: The following screen opens. In the Settings and service screen, tap Sample valve. Result: The Sample valve screen opens. Check that the Valve position Buffer is selected.
  • Page 87 5 Service and settings 5.7 Sample valve Step Action Stop the Pump: In the Settings and service screen, access Pump:Diagnostics screen. • Stop the Pump by tapping Stop flow. • If these checks indicate that the valve is faulty, replace the Sample valve. If the valve has been replaced, tap Reset to set the Valve switches counter to 0.

  • Page 88: Wash Valve

    5 Service and settings 5.8 Wash valve Wash valve Description The Wash valve can be switched to divert the flow either to column or to waste. Parameter Description Valve switches Indicates how many times the valve has switched. • When an old valve has been replaced, the number of •...
  • Page 89 5 Service and settings 5.8 Wash valve Step Action In the Settings and service screen, tap Next to access the 2 screen. Result: The following screen opens. In the Settings and service screen, tap Wash valve. Result: The Wash valve screen opens. Check that the valve position Waste is selected.
  • Page 90 5 Service and settings 5.8 Wash valve Step Action If the valve has been replaced, tap Reset to set the Valve switches counter to 0. Result: A confirmation screen opens. Tap Yes to confirm the reset if the valve has been replaced. If the valve has not been replaced, tap No to cancel the action.

  • Page 91: Outlet Valve

    5 Service and settings 5.9 Outlet valve Outlet valve Description The Outlet valve can be switched to divert the flow, either to waste or to the Fraction collector. Parameter Description Valve switches Indicates how many times the valve has switched. •...
  • Page 92 5 Service and settings 5.9 Outlet valve Step Action In the Settings and service screen, tap Next to access the 2 screen. Result: The following screen opens. In the Settings and service screen, tap Outlet valve. Result: The Outlet valve screen opens. Check that the valve position Waste is selected.
  • Page 93 5 Service and settings 5.9 Outlet valve Step Action If these checks indicate that the valve is faulty, replace the Outlet valve. If the valve has been replaced, tap Reset to set the Valve switches counter to 0. Result: A confirmation screen opens. Tap Yes to confirm the reset if the valve has been replaced.

  • Page 94: Conductivity Monitor

    5 Service and settings 5.10 Conductivity Monitor 5.10 Conductivity Monitor Introduction This section describes how to perform Conductivity Monitor calibrations and edit the Cell constant settings. In this section This section contains the following sub-sections: Section See page 5.10.1 Set the Cell constant value 5.10.2 Temperature sensor calibration 5.10.3 Conductivity flow cell calibration Description...

  • Page 95: Set The Cell Constant Value

    5 Service and settings 5.10 Conductivity Monitor 5.10.1 Set the Cell constant value 5.10.1 Set the Cell constant value Instructions If the flow cell has been replaced, a new cell constant value has to be set. Follow the in- structions below to set the Cell constant value. Step Action In the Settings and service screen, tap Next:Next to access the 3...
  • Page 96 5 Service and settings 5.10 Conductivity Monitor 5.10.1 Set the Cell constant value Step Action Set the Cell constant value (Set cell const) for the new Conductivity flow cell by using the up/down arrows. Tap Save to save the new value. Note: •...

  • Page 97: Temperature Sensor Calibration

    Temperature sensor calibration Instructions Follow the instructions below to calibrate the temperature sensor. NOTICE The option Calibrate sine gen must be performed by a GE Service Engineer. Step Action Place a precision thermometer in the flow cell path directly after the Con- ductivity flow cell (i.e., immediately after the conductivity tubing which is...

  • Page 98: Conductivity Flow Cell Calibration

    5 Service and settings 5.10 Conductivity Monitor 5.10.3 Conductivity flow cell calibration 5.10.3 Conductivity flow cell calibration Prerequisites Calibration solution: • 1.00 M NaCl • 100 mS/cm conductivity standard solution Instructions Follow the instructions below to calibrate the Conductivity flow cell. Note: Temperature compensation is enabled by default (factory setting).
  • Page 99 5 Service and settings 5.10 Conductivity Monitor 5.10.3 Conductivity flow cell calibration Step Action Note the current temperature of the calibration solution in the Conductivity flow cell as displayed in the Cell Temperature field. In the Calibration screen, enter the conductivity value at the current tem- perature in the Set Conductivity field and then tap Calibrate to carry out the Conductivity calibration.
  • Page 100 5 Service and settings 5.10 Conductivity Monitor 5.10.3 Conductivity flow cell calibration Step Action Axis Description Temperature (°C) Conductivity (mS/cm) ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 101: Pressure Sensor

    Follow the instructions below to set the pressure to 0 when the Pressure sensor is ex- posed to atmospheric pressure only (Zero offset). NOTICE Calibration of Pressure sensor must be performed by a GE Service Engineer, and must be done when the Pressure sensor has been replaced.
  • Page 102 5 Service and settings 5.11 Pressure sensor Step Action In the Settings and service screen, tap Pressure sensor. Result: The Pressure sensor screen opens. In the Pressure sensor screen, tap Zero offset. Result: A Message screen opens. Make sure that there is no back pressure in the system, and then tap OK. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 103: Frac30

    5 Service and settings 5.12 Frac30 5.12 Frac30 Introduction This section describes how to enable or disable Frac30, perform diagnostics and handle the run log. In this section This section contains the following subsections: Section See page 5.12.1 Enable or disable Frac30 5.12.2 Diagnostics 5.12.3 Run Log ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 104: Enable Or Disable Frac30

    5 Service and settings 5.12 Frac30 5.12.1 Enable or disable Frac30 5.12.1 Enable or disable Frac30 Instructions Follow the instructions below to enable or disable Frac30. Step Action In the Settings and service screen, tap Fraction collector. Result: If the Fraction collector is enabled, the following Fraction collector screen opens.

  • Page 105: Diagnostics

    5 Service and settings 5.12 Frac30 5.12.2 Diagnostics 5.12.2 Diagnostics Parameter description Parameter Description Feed tube test Checks that Frac30 rotates the Bowl assembly correctly and shifts one tube at a time. Home test Checks that Frac30 rotates the Bowl assembly correctly and shifts from the current position to the home position (tube number 1).
  • Page 106 5 Service and settings 5.12 Frac30 5.12.2 Diagnostics Step Action Tap Home test and visually observe that Frac30 rotates to home position (tube no. 1) from current position. Note: If Frac30 does not rotate the Bowl assembly, check if the Frac30 cable is connected properly to ÄKTA start.

  • Page 107: Run Log

    5 Service and settings 5.12 Frac30 5.12.3 Run Log 5.12.3 Run Log Parameter description Parameter Description Run log Displays the number of hours of drive sleeve usage. Instructions Follow the instructions below to reset the Run log. Step Action In the Fraction collector screen, tap Run log. Result: The Run log screen opens.
  • Page 108 5 Service and settings 5.12 Frac30 5.12.3 Run Log Step Action If the drive sleeve has been replaced, tap Reset to set the Run time to 0. Result: A confirmation screen opens. Tap Yes to confirm the reset if the drive sleeve has been replaced. If the drive sleeve has not been replaced, tap No to cancel the action.

  • Page 109: Instrument Display

    5 Service and settings 5.13 Instrument Display 5.13 Instrument Display Introduction This section describes how to calibrate and test the Instrument Display. In this section This section contains the following subsections: Section See page 5.13.1 Touch screen calibration 5.13.2 Color test 5.13.3 Diagnostics 5.13.4 Log book Instructions...
  • Page 110 5 Service and settings 5.13 Instrument Display Step Action Tap Display to access the Instrument Display options. Result: The Display screen opens. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 111: Touch Screen Calibration

    5 Service and settings 5.13 Instrument Display 5.13.1 Touch screen calibration 5.13.1 Touch screen calibration Instructions Follow the instructions below to calibrate the Touch screen. Step Action In the Display screen, tap Touch screen calibration. Result: A warning screen opens. Tap Yes to proceed with the calibration of the Touch screen.
  • Page 112 5 Service and settings 5.13 Instrument Display 5.13.1 Touch screen calibration Step Action Tap precisely on the marked circle 1. Result: The following screen opens. Tap precisely on the marked circle 2. Result: The following screen opens. Tap precisely on the marked circle 3. Result: The following screen opens.
  • Page 113 Step Action Tap precisely on the marked circle 4. Result: The following screen opens. Tap Exit. Note: If the calibration fails, repeat the test. If the calibration fails again, contact a GE Service Engineer. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 114: Color Test

    Follow the instructions below to test the colors of the Touch screen. Step Action In the Display screen, tap Color test. Result: The following screen opens. Tap Exit. Result: The Color test is completed. Note: If the test fails, contact a GE Service Engineer. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 115: Diagnostics

    5 Service and settings 5.13 Instrument Display 5.13.3 Diagnostics 5.13.3 Diagnostics Instructions Follow the instructions below to perform diagnostics of the Touch screen. Step Action In the Display screen, tap Diagnostics. Result: The following screen opens. In the Select backlight brightness screen, tap a radio button to select the intended brightness (%).

  • Page 116: Log Book

    Instrument Display has been replaced, it is required to reset to 0 the number of hours of display usage. Note: The instrument display must be replaced by a GE Service Engineer. Instructions Follow the instructions below to read the Log book for the Display.
  • Page 117 5 Service and settings 5.13 Instrument Display 5.13.4 Log book Step Action If the Instrument Display has been replaced, tap Reset to set the number of hours to 0. Result: A confirmation screen opens. Tap Yes to confirm the reset if the Instrument Display has been replaced. If the Instrument Display has not been replaced, tap No to cancel the action.

  • Page 118: System

    5 Service and settings 5.14 System 5.14 System Introduction This section describes how to handle firmware updates and export system reports. It also describes how to set delay volumes and switch valve timing. In this section This section contains the following subsections: Section See page 5.14.1 Firmware update...
  • Page 119 4 error messages with Error codes, to a USB memory stick. The latest Firmware ver- sion and FPGA version can also be exported. The data is used by a GE Service Engineer when troubleshooting the instrument. Instructions Follow the instructions below to manage the system options.

  • Page 120: Firmware Update

    5 Service and settings 5.14 System 5.14.1 Firmware update 5.14.1 Firmware update NOTICE Before tapping the Firmware update ensure that the system is connected to a stable source of power such as a UPS. During firmware update the system should not be switched off. Prerequisites •...
  • Page 121 5 Service and settings 5.14 System 5.14.1 Firmware update Step Action Tap Microprocessor firmware update. Result: The following screen opens. Tap Yes and then wait for approximately 3 minutes for the update to be done. Note: When the firmware update is completed, the instrument automatically restarts and displays the version of the firmware.
  • Page 122 5 Service and settings 5.14 System 5.14.1 Firmware update Step Action Once the firmware is updated, perform the following calibration sequence: Display:Touch screen calibration • Pressure sensor:Zero offset • Pump : Calibration:Flow rate • UV:UV LED calibration • Conductivity:Advanced calibration (temperature sensor calibration) •...

  • Page 123: Export System Report To Usb

    Export system report to USB Instructions Follow the instructions below to export the system report to a USB memory stick. Use the system report in further contacts with GE Service Engineers. Step Action Plug the USB memory stick into the USB port located on ÄKTA start.

  • Page 124: Delay Volume Setting

    5 Service and settings 5.14 System 5.14.3 Delay volume setting 5.14.3 Delay volume setting Instructions Follow the instructions below to set the delay volume. Step Action In the System screen, tap Delay volume setting. Result: The following screen opens. Enter the internal diameter (ID) and length of the tubing from the Outlet valve to Frac30 in the respective fields, and then tap Save.

  • Page 125: Switch Valve Timing

    5 Service and settings 5.14 System 5.14.4 Switch valve timing 5.14.4 Switch valve timing Instructions Follow the instructions below to set the Switch valve timing. For more details, refer to ÄKTA start Operating Instructions section Switch valve timing. Step Action In the Settings and service screen, tap System.
  • Page 126 5 Service and settings 5.14 System 5.14.4 Switch valve timing Step Action If wavy gradients still are obtained, or if fluctuations on step gradient levels are large, then select Advanced timing. Result: The following screen opens. Set switch valve time in the range of 3.0 to 5.0 sec (0.1 sec increments) by pressing the up/down arrows.

  • Page 127: Main Board

    NOTICE The Main board screen is reserved for operations performed by a GE Service Engineer only. Do not perform any further operations from this screen. Tap Back or Home to return to the previous screen or to go to the Home screen.

  • Page 128: Troubleshooting

    6 Troubleshooting Troubleshooting About this chapter This chapter contains information regarding troubleshooting procedures. Warning messages and Error codes are provided, and possible causes and solutions to those codes are described. In this chapter This chapter contains the following sections: Section See page 6.1 UV 6.2 Conductivity...
  • Page 129 6 Troubleshooting 6.1 UV Warning messages Warning Descrip- Possible cause Action code tion UV intensi- In the Settings and ser- Flush the UV flow cell • ty low vice:UV screen: and mount it in a se- cure way When trying to calibrate; if the detector voltage is less than 2500 mV, even Replace the UV flow...
  • Page 130 6 Troubleshooting 6.1 UV Warning Descrip- Possible cause Action code tion In the Settings and ser- Order a new UV Moni- • reached vice:UV screen: tor. end of life- When trying to save the Perform UV LED cali- • time UV light strength after bration.
  • Page 131 6 Troubleshooting 6.1 UV Error code Descrip- Possible cause Action tion UV module During power up, stray failure light test has failed, light Troubleshooting Flow is "leaking" in to the detec- chart 2, on page 140 tor. UV module UV module cable may be failure loose or disconnected, no Troubleshooting Flow...

  • Page 132: Conductivity

    6 Troubleshooting 6.2 Conductivity Conductivity Error messages Error code Descrip- Possible cause Action tion Conductivi- Loose cable connec- • ty module tor. Troubleshooting Flow failure chart 6, on page 144 The temperature sen- • sor is not functioning. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 133: Frac30

    6 Troubleshooting 6.3 Frac30 Frac30 Error messages Error code Descrip- Possible cause Action tion Fraction The cable connected • collector between ÄKTA start Troubleshooting Flow failure and Frac30 is not chart 4, on page 142 working. Drive sleeve worn out. •...

  • Page 134: Pump

    Pump Error messages Error code Descrip- Possible cause Action tion Pump fail- Pump is not working. Contact a GE Service • • Engineer. • Troubleshooting Flow chart 5, on page 143 No flow The rollers are not rotat- Check the condition of •...

  • Page 135: Pressure Sensor

    • tion and pressure of the columns for cor- rect flow rate. Pressure The sensor is not connect- Perform Zero offset. • sensor fail- ed or not calibrated. Contact a GE Service • Engineer. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 136: Main Board And Power Supply

    Error messages Error code Descrip- Possible cause Action tion EPROM er- Contact a GE Service • Engineer. MPWAtem- Instrument tempera- Contact a GE Service • • perature ture is way out of Engineer.
  • Page 137 6 Troubleshooting 6.6 Main board and Power Supply Error code Descrip- Possible cause Action tion Power sup- Voltage has gone out of Contact a GE Service • ply monitor operating range. Engineer. error With assistance from • a GE Service Engineer, check the Power sup- ply for voltages.

  • Page 138: System Related Error Messages

    6 Troubleshooting 6.7 System related error messages System related error messages Error messages Error code Descrip- Possible cause Action tion Method Er- Incomplete method. Reload the method. ror. Reload Wrong method loaded. Method Illegal op- Incomplete method. Reload the method. code Wrong method loaded.

  • Page 139: Troubleshooting Flow Charts

    1. Switch the instrument Off. 2. Disassemble the UV Monitor. 3. Replace the UV Monitor and Flow cell. 4. Switch instrument On. Error message still Perform UV LED calibration displayed? Contact GE Service Engineer. Proceed with your work. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 140 1. Switch instrument Off. 2. Disassemble the UV Monitor. 3. Replace the UV Monitor and Flow cell. 4. Switch instrument On. Error message Perform UV LED still displayed? calibration Contact GE Service Engineer. Proceed with your work. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 141 2. Disassemble the UV Monitor. 3. Check that the UV Monitor RJ45 cable is secure; replug properly. 4. Switch on the instrument. Error message Contact GE Service Engineer. still displayed? Proceed with your work. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 142 Replace the Drive sleeve. (Check) Run Frac30 diagnostics. Perform indexing of homing to check if Motor works properly. Does the Motor Contact GE Service Engineer. work properly? Error message Contact GE Service Engineer. still displayed? Proceed with your work. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 143 6 Troubleshooting 6.8 Troubleshooting flow charts Troubleshooting Flow chart 5 ERROR 401 Pump driver Failure Contact GE Service Engineer ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 144 Switch on the instrument. 1. Replace the Conductivity Monitor. 2. Enter the new Cell Constant value. Error message still 3. Perform Conductivity calibration. displayed? Has calibration Proceed with your work. passed? Contact GE Service Engineer. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 145: Disassembly

    7 Disassembly Disassembly About this chapter This chapter contains instructions how to disassemble, remove and replace all the modules that can be disassembled by the user. In this chapter This chapter contains the following sections: Section See page 7.1 Buffer valve 7.2 Mixer 7.3 UV 7.4 UV flow cell...
  • Page 146 NOTICE Replacement of modules located on the inside of the instrument must be performed by a GE Service Engineer only. If an internal part needs to be replaced, please contact a GE Service Engineer. Internal modules include: •...
  • Page 147 7 Disassembly Illustration of the modules The illustration below shows the locations of the modules placed on the wet side of the instrument. Part Module Disassembly instructions Buffer valve Instructions, on page 149 Mixer Instructions, on page 153 Sample valve Instructions, on page 163 Pump Instructions, on page 167...
  • Page 148 7 Disassembly Part Module Disassembly instructions Conductivity Instructions, on page 182 Outlet valve Instructions, on page 179 ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 149: Buffer Valve

    7 Disassembly 7.1 Buffer valve Buffer valve Note: In case of multiple valve failures, make sure that the valves are not mixed. Replace the valves one by one by removing one valve at a time from the instru- ment. Required tools Tool Dimension Torx driver...
  • Page 150 7 Disassembly 7.1 Buffer valve Step Action Loosen the M3 screw from the valve using a T10 torx driver, supplied with the equipment at delivery. Note: The screw can be located on different places on the top circumference of the holder.
  • Page 151 7 Disassembly 7.1 Buffer valve Step Action Press on the connector and remove the connector and cable. Disconnect the connector from the valve side. Remove the valve and leave the connector as it is. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 152 7 Disassembly 7.1 Buffer valve Step Action Replace with a new Buffer valve, connect the cable, and reconnect the tubing to the valve ports. Note: Before replacing the valve, make sure that the part number (P/N) on the new valve matches with that of the old valve (29003271, Buffer valve CTV-31- 516U-1).

  • Page 153: Mixer

    7 Disassembly 7.2 Mixer Mixer Required tools Tool Dimension Torx screwdriver Instructions Follow the instructions below to remove and replace the Mixer. WARNING Disconnect power. Always switch off power to the instrument be- fore replacing any component on the instrument or cleaning the instrument, unless stated otherwise in the user documentation.
  • Page 154 7 Disassembly 7.2 Mixer Step Action Loosen the M4 screw from the Mixer using a T20 screwdriver, supplied with the equipment at delivery. Remove the Mixer. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 155 7 Disassembly 7.2 Mixer Step Action Replace with a new Mixer and reconnect the tubing to the ports. Switch on the instrument by pressing the Power Switch to the l position. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 156 7 Disassembly 7.3 UV Required tools Tool Dimension Torx screwdriver Instructions Follow the instructions below to remove and replace the UV Monitor. WARNING Disconnect power. Always switch off power to the instrument be- fore replacing any component on the instrument or cleaning the instrument, unless stated otherwise in the user documentation.
  • Page 157 7 Disassembly 7.3 UV Step Action Unscrew the one screw at the top of the UV Monitor, and then the two screws at the bottom using a T20 screwdriver, supplied with the equipment at deliv- ery. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 158 7 Disassembly 7.3 UV Step Action Slowly remove the UV Monitor until you get access to the RJ45 connector plugged into the module. Press the latching tab and disconnect the RJ45 connector from the UV Monitor side. Note: Make sure that the RJ45 connector is not moved inside the cabinet. The con- nector needs to be retained outside the cabinet for re-assembling of a new UV module.
  • Page 159 7 Disassembly 7.3 UV Step Action Remove the UV Monitor and leave the RJ45 connector as it is. Connect the RJ45 connector to the new UV monitor. Note: Make sure that there is a click sound when the RJ45 connector is connected. The UV monitor may not work properly if the RJ45 connector is not properly connected.
  • Page 160 7 Disassembly 7.3 UV Step Action Reconnect the tubing to the ports. Switch on the instrument by pressing the Power Switch to the l position. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 161: Uv Flow Cell

    7 Disassembly 7.4 UV flow cell UV flow cell Instructions Follow the instructions below to remove and replace the UV flow cell. Step Action Disconnect the inlet and outlet tubing from the UV Monitor. Rotate the locknut in the anticlockwise direction. Pull up the UV flow cell.
  • Page 162 7 Disassembly 7.4 UV flow cell Step Action Place the protective cover around the flow cell to protect the electronics in- side the optical unit from liquid spillage. Note: The protective cover should be assembled after completing the assembly of the UV flow cell inside the UV Monitor by just press-fit.

  • Page 163: Sample Valve

    7 Disassembly 7.5 Sample valve Sample valve Required tools Tool Dimension Torx driver Instructions Follow the instructions below to remove and replace the Sample valve. WARNING Disconnect power. Always switch off power to the instrument be- fore replacing any component on the instrument or cleaning the instrument, unless stated otherwise in the user documentation.
  • Page 164 7 Disassembly 7.5 Sample valve Step Action Loosen the screw from the valve using a T10 torx driver, supplied with the equipment at delivery. Note: The screw can be locate on different places on the top circumference of the holder. Slowly remove the Sample valve until a cable is visible.
  • Page 165 7 Disassembly 7.5 Sample valve Step Action Disconnect the connector from the valve side. Remove the Sample valve. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 166 7 Disassembly 7.5 Sample valve Step Action Replace with a new Sample valve, connect the cable, and reconnect the tubing to the valve ports. Note: Before replacing the valve, make sure that the part number (P/N) on the new valve matches with that of the old valve (29003272, Sample valve CTV-31- 32U-3).

  • Page 167: Pump

    7 Disassembly 7.6 Pump Pump Required tools Tool Dimension Torx driver Instructions Follow the instructions below to remove and replace the Pump. WARNING Disconnect power. Always switch off power to the instrument be- fore replacing any component on the instrument or cleaning the instrument, unless stated otherwise in the user documentation.
  • Page 168 7 Disassembly 7.6 Pump Step Action Open the top cover to remove the tubing from the Pump. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 169 7 Disassembly 7.6 Pump Step Action Loosen the two M4 screws from the Pump using a T20 screwdriver, supplied with the equipment at delivery. Note: Make sure that the pump connector is not moved inside the cabinet. The connector needs to be retained outside the cabinet for re-assembling of a new Pump module.
  • Page 170 7 Disassembly 7.6 Pump Step Action Slowly remove the Pump until a cable is visible. The cable is assembled with two connectors interconnected. Disconnect the connector from the Pump side. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 171 7 Disassembly 7.6 Pump Step Action Remove the Pump. Note: Make sure that the pump cable is placed away from the fan, on the left side of the instrument after removing the Pump. This safety precautions is to ensure that the cable does not get stuck in the ventilation fan. Replace with a new Pump, install the new Pump in reverse order.

  • Page 172: Pump Tubing

    7 Disassembly 7.7 Pump tubing Pump tubing Instructions Follow the instructions below to remove and replace the pump tubing. WARNING Disconnect power. Always switch off power to the instrument be- fore replacing any component on the instrument or cleaning the instrument, unless stated otherwise in the user documentation.
  • Page 173 7 Disassembly 7.7 Pump tubing Step Action Place the tubing between the rollers and the track, press against the pump head inner wall. Note: Make sure that the pump tubing is not twisted or stretched against the rollers. Lower the top cover until it clicks into its fully closed position. The track closes automatically and the tubing is stretched correctly as the track closes.
  • Page 174 7 Disassembly 7.7 Pump tubing Step Action Connect the pump tubing to the Sample valve and to the Pressure sensor. Switch on the instrument by pressing the Power Switch to the l position. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 175: Wash Valve

    7 Disassembly 7.8 Wash valve Wash valve Required tools Tool Dimension Torx driver Instructions Follow the instructions below to remove and replace the Wash valve. WARNING Disconnect power. Always switch off power to the instrument be- fore replacing any component on the instrument or cleaning the instrument, unless stated otherwise in the user documentation.
  • Page 176 7 Disassembly 7.8 Wash valve Step Action Loosen the M3 screw from the Wash valve using a T10 torx driver, supplied with the equipment at delivery. Note: The screw can be located on different places on the top circumference of the holder.
  • Page 177 7 Disassembly 7.8 Wash valve Step Action Disconnect the connector from the valve side. Remove the Wash valve from the instrument. Replace with a new Wash valve. Connect the cable, and then reconnect the tubing to the valve ports. Note: Before replacing the valve, make sure that the part number (P/N) on the new valve matches with that of the old valve (29014870 AA Wash valve CTV-3- 32UH-2).
  • Page 178 7 Disassembly 7.8 Wash valve Step Action Switch on the instrument by pressing the Power Switch to the l position. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 179: Outlet Valve

    7 Disassembly 7.9 Outlet valve Outlet valve Required tools Tool Dimension Torx driver Instructions Follow the instructions below to remove and replace the Outlet valve. WARNING Disconnect power. Always switch off power to the instrument be- fore replacing any component on the instrument or cleaning the instrument, unless stated otherwise in the user documentation.
  • Page 180 7 Disassembly 7.9 Outlet valve Step Action Loosen the M3 screw from the Outlet valve using a T10 torx driver, supplied with the equipment at delivery. Note: The screw can be located on different places on the top circumference of the holder.
  • Page 181 7 Disassembly 7.9 Outlet valve Step Action Disconnect the connector from the valve side. Remove the Outlet valve. Replace with a new Outlet valve. Connect the cable, and then reconnect the tubing to the valve ports. Note: Before replacing the valve, make sure that the part number on the new valve matches with that of the old valve (29003274 Outlet valve CTV-31-32U-1).

  • Page 182: Conductivity Monitor

    7 Disassembly 7.10 Conductivity Monitor 7.10 Conductivity Monitor Required tools Tool Dimension Torx screwdriver Instructions Follow the instructions below to remove and replace the Conductivity Monitor. WARNING Disconnect power. Always switch off power to the instrument be- fore replacing any component on the instrument or cleaning the instrument, unless stated otherwise in the user documentation.
  • Page 183 7 Disassembly 7.10 Conductivity Monitor Step Action Loosen the M4 screws from the Conductivity Monitor using a T20 screwdriv- er, supplied with the equipment at delivery. Slowly remove the Conductivity Monitor until a cable is visible. The cable is assembled with two connectors interconnected. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 184 7 Disassembly 7.10 Conductivity Monitor Step Action Disconnect the connector from the Conductivity Monitor side. Remove the Conductivity Monitor and leave the connector as it is. Note: Make sure that the connector is not moved inside the cabinet. The connector needs to be retained outside the cabinet for re-assembling of a new Conduc- tivity module.
  • Page 185 7 Disassembly 7.10 Conductivity Monitor Step Action Replace with a new Conductivity Monitor and reconnect the tubing to the ports. Switch on the instrument by pressing the Power Switch to the l position. In the Settings and service screen, access the Conductivity screen to set the cell constant for the new Conductivity flow cell.

  • Page 186: Injection Valve

    7 Disassembly 7.11 Injection valve 7.11 Injection valve Required tools Tool Dimension Torx driver Instructions Follow the instructions below to remove and replace the Injection valve. WARNING Disconnect power. Always switch off power to the instrument be- fore replacing any component on the instrument or cleaning the instrument, unless stated otherwise in the user documentation.
  • Page 187 7 Disassembly 7.11 Injection valve Step Action Loosen the M3 screw from the Injection valve using a T10 torx driver, sup- plied with the equipment at delivery. Remove the Injection valve. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 188 7 Disassembly 7.11 Injection valve Step Action Replace with a new Injection valve and reconnect the tubing to the ports. Make sure that the ports are aligned to the markings on the instrument chassi. Switch on the instrument by pressing the Power Switch to the l position. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 189: Injection Valve Kit

    7 Disassembly 7.12 Injection valve kit 7.12 Injection valve kit Required tools Tool Dimension Torx driver Instructions Follow the instructions below to remove and replace the Injection valve kit. Step Action Make sure that the valve is in position: Inject, and then disconnect the inlet and outlet tubing from the ports.
  • Page 190 7 Disassembly 7.12 Injection valve kit Step Action Remove the 3 screws on the front side, using the supplied torx driver. Loosen each screw equally in turn, so the distribution plate comes off in parallel to the valve body. Slide the screws out. Remove the distribution plate containing the ports.
  • Page 191 7 Disassembly 7.12 Injection valve kit Step Action Remove the old channel plate and insert a new one. Remount a new distribution plate so that the marks on the plate match the marks on ÄKTA start. Using the torx driver, tighten the 3 screws in turn, a little at a time, until the distribution plate is fixed to the valve body.

  • Page 192: Frac30 Bowl Assembly

    7 Disassembly 7.13 Frac30 Bowl assembly 7.13 Frac30 Bowl assembly Remove the Bowl assembly Follow the instructions below to remove the Frac30 Bowl assembly. Step Action Gently move the Dispenser arm counterclockwise to the non-dispensing (end) position. Push the drive assembly laterally and hold it at the retracted position. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 193 7 Disassembly 7.13 Frac30 Bowl assembly Step Action Lift and remove the Bowl assembly. Mount the Bowl assembly onto the Bowl holder Follow the instructions below to mount the Frac30 Bowl assembly onto the Bowl holder. Step Action Make sure that the Dispenser arm is in the non-dispensing position. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 194 7 Disassembly 7.13 Frac30 Bowl assembly Step Action Note the position of the aligning groove on the Bowl holder. Note the position of the aligning ribs on the Bowl. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 195 7 Disassembly 7.13 Frac30 Bowl assembly Step Action Hold the Bowl assembly and orient the Bowl with the aligning ribs oriented towards the aligning groove on the Bowl holder. Note: Do not lift the fraction collector by holding the Dispenser arm. Tip: If it is hard to locate the alignment feature in corresponding parts, hold the Bowl assembly near the top of the Bowl holder and rotate the Bowl holder...
  • Page 196 7 Disassembly 7.13 Frac30 Bowl assembly Step Action Hold the Drive assembly at the retracted position to completely assemble the Bowl assembly on the Bowl holder. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 197 7 Disassembly 7.13 Frac30 Bowl assembly Remove and fix the drive sleeve Follow the instructions below to remove and replace the drive sleeve. Step Action Cut the old drive sleeve with a cutter, and then remove the drive sleeve. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 198 7 Disassembly 7.13 Frac30 Bowl assembly Step Action Mount a new drive sleeve by pressing and sliding it onto the drive. Remove and fix the Tubing holder Follow the instructions below to remove and replace the Tubing holder. Step Action Pull out the Tubing holder from the Dispenser arm.
  • Page 199 7 Disassembly 7.13 Frac30 Bowl assembly Step Action Press down the new Tubing holder into the Dispenser arm until it is stopped by the dispensing arm stopping feature. ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 200: Fuse

    7 Disassembly 7.14 Fuse 7.14 Fuse Required tools Tool Dimension Flat screwdriver Remove the Fuse Follow the instructions below to remove the Fuse. Step Action Use the flat screwdriver provided, and push the Snap feature on the left side of the Fuse holder, in the direction indicated by the arrow mark. ÄKTA start Maintenance Manual 29-0603-08 AC...
  • Page 201 7 Disassembly 7.14 Fuse Step Action Use the flat screwdriver, and push the Snap feature on the right side of the Fuse holder, in the direction indicated by the arrow mark. It should now be possible to slide out the Fuse from the holder, pull out the Fuse by hand.
  • Page 202 7 Disassembly 7.14 Fuse Mount the Fuse Follow the instructions below to replace the Fuse. Step Action Align the Fuse holder guide feature to the rectangular slot of the Mains filter. Push the Fuse holder into the rectangular slot until it fits into the rectangular groove of the Mains filter.

  • Page 203: Reference Information

    8 Reference information Reference information About this chapter This chapter includes Health and Declaration forms for service, and ordering information for ÄKTA start. In this chapter Section See page 8.1 Health and Safety Declaration Form 8.2 Ordering information ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 204: Health And Safety Declaration Form

    Declar ration F Form Serv vice Ticket # To ma ke the mutual protection an nd safety of GE E service perso onnel and our c customers, all equipment an nd work areas must be clean a and free of any...
  • Page 205 GE. To avoid d delays in the p rocessing of your e quipment, ple ase complete this checklist and include it with your retu urn.

  • Page 206: Ordering Information

    8 Reference information 8.2 Ordering information Ordering information For ordering information visit www.gelifesciences.com/AKTA. Accessories list Part Accessory description Code no. Pump Marprene Tubing 29-0240-12 Peristaltic Pump 29-0239-92 Solenoid valve Buffer valve 29-0238-95 Sample valve 29-0238-96 Wash valve 29-0238-97 Outlet valve 29-0238-98 Manual Injection valve Injection valve, Manual...

  • Page 207: Ordering Information

    8 Reference information 8.2 Ordering information Part Accessory description Code no. Fittings Tubing Connector 1/8" 18-1121-17 Ferrule for 1/8" tubing 18-1121-18 Union Luer Female/HPLC Male 18-1112-51 Fingertight Connector 1/16" 18-1112-55 Stop plug 1/16", PKG/5 18-1112-52 Stop plug, 5/16", PKG/5 18-1112-50 Union, 1/16"...
  • Page 208 8 Reference information 8.2 Ordering information Part Accessory description Code no. Miscellaneous Inlet filter assembly 18-1113-15 Inlet filter set, 10 Filters/Nets 18-1114-42 Screw lid GL45 kit, ÄKTA 11-0004-10 Tubing cutter 18-1112-46 Column clamp o.d. 10 to 21 mm 28-9563-19 Short column holder 18-1113-17 T-Slot holders 29-0240-38...
  • Page 209 8 Reference information 8.2 Ordering information Service tools Item Code no. Torx driver T10 29-0031-71 Torx driver T20 28-9513-03 Flat screwdriver 56-4656-00 ÄKTA start Maintenance Manual 29-0603-08 AC...

  • Page 210: Index

    Index Index remove bowl assembly, 192 reset Run log, 107 ÄKTA start settings and diagnos- instrument main parts, 36 tics, 103 instrument modules, descrip- Fuse tions, 39 replace, 200 instrument modules, illustra- tion, 59 intended use, 35 Injection valve main features, 38 remove and replace, 186 system description, 35 remove and replace kit, 189...
  • Page 211 Index warning messages, 136 System Pressure sensor error messages, 138 calibrations, 101 export system report, 123 error messages, 135 firmware updates, 120 Pump set Switch valve timing, 125 calibration, 78 set the delay volume, 124 diagnostics, 76 error messages, 134 remove and replace, 167 Troubleshooting, 128 remove and replace tub-...
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  • Page 214 751 84 Uppsala All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms Sweden and conditions is available on request. Contact your local GE Healthcare repre- sentative for the most current information.

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