GE ÄKTA start Manual
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GE ÄKTA start Manual

GE ÄKTA start Manual

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Table of Contents

See also: Maintenance Manual
ÄKTA ™ start
System Cue Card
System Overview
ÄKTA start is a simple and modern liquid chromatography system intended for
preparative purification of proteins at laboratory scale.
The system can be used for a variety of research purposes to fulfill the needs of the
users in the academia and in the life sciences industry. ÄKTA start is intended for
research use only, and should not be used in any clinical procedures, or for diagnostic
purposes.
The System Cue Card describes how to prepare the system for a chromatography run,
start and monitor a run, and evaluate the result.
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  • Page 1: Table Of Contents

    ÄKTA ™ start System Cue Card Contents System overview 2 System preparation 4 Column preparation 6 Starting a run 6 Monitoring a run 7 Quick start methods 8 Flow charts 10 Getting help 11 Reference information 11 System Overview ÄKTA start is a simple and modern liquid chromatography system intended for preparative purification of proteins at laboratory scale.

  • Page 2: System Overview

    System overview Create method: create, edit, import and delete methods Part Description Settings and service: configure, calibrate, troubleshoot ÄKTA start and diagnose modules Frac30, Fraction collector Instrument illustration PC with UNICORN™ start software The illustration below shows the locations of the modules placed on the wet side of the instrument.
  • Page 3 Frac30, Fraction collector • Evaluating and analyzing the results from ÄKTA start Module Features System Provides an intuitive and easy-to-use interface to control ÄKTA start. Control Part Description Part Description Performs and monitors manual Dispenser arm Bowl assembly chromatography runs. Tubing holder Base unit Performs and monitors automated...

  • Page 4: System Preparation

    System preparation Instrument Fractionation options configuration ÄKTA start + • Fixed volume fractionation Preparation workflow UNICORN start + • Peak fractionation Frac30 Instruction ◊ Level based Place the buffer bottles (A & B) in the buffer tray and ◊ Slope based immerse the buffer inlet tubing (A &...
  • Page 5 Prefill the flow path larger sample volumes (10 - 150 ml), use a Superloop™. The loop has to be connected to the Injection valve Cleaning/prefilling the flow path including all inlets and (ports 2 and 5) as depicted below. outlets is performed using the system methods listed below. For instructions on how to prime the sample inlet tubing and sample loop, refer to ÄKTA start Operating Instructions, section 5.7 Sample application.

  • Page 6: Column Preparation

    Starting a run Tighten all connections and pump DM water through the system to check for leaks. Tighten any leaking connector. Chromatography runs can be performed either by running Removal from cold room predefined methods, or by using manual operations. Switch off the instrument and disconnect power cable.

  • Page 7: Monitoring A Run

    Perform a manual run Import a method to ÄKTA start Follow the workflows below to run the system manually. Transfer the UNICORN method to USB memory stick, then perform the following operations to import a method. Operation from the Instrument Display The imported methods are saved in User defined methods menu on the ÄKTA start.

  • Page 8: Quick Start Methods

    Evaluating a run Note: The result files will be saved in the GE folder which is automatically created by the instrument once the USB Save Result as BMP image memory stick is plugged in. Only 10 results can be stored in the GE folder.
  • Page 9 The pre-set run parameters for each technique are listed in the table below. Parameter AC step AC step DS 5 ml IEX step IEX step 1 ml 5 ml HiTrap 53 ml 1 ml 5 ml gradient gradient 16/60 HiTrap HiTrap HiPrep™...

  • Page 10: Flow Charts

    Flow charts The active flow path is depicted in Green color. Flow path details for flow of 100% buffer A through Outlet valve to waste Fractionation/collection 3 CV 1.2 CV IEX Step 1 ml/5 ml HiTrap - Ion Exchange Chromatography The bound proteins are eluted in a single step using single elution buffer.

  • Page 11: Getting Help

    • Accessible within UNICORN start by pressing F1 or using the Help menu. • The online help covers the system control, method editor and result evaluation functions in UNICORN start. The user documentation available with ÄKTA start is listed in section Reference information. Reference information Ordering information Flow path details, fraction collection...
  • Page 12 All goods and services are sold subject to the terms and conditions elution from CIEX 20 mM MES pH 6.0, 1 M NaCl of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact GF buffer suggestions your local GE Healthcare representative for the most current information.

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